Differential mRNA expression of genes in the porcine adrenal gland associated with psychosocial stress

E Murani, S Ponsuksili, RB D'Eath, SP Turner, G Evans, L Thoelking, E Kurt, R Klont, A Foury, P Mormede, K Wimmers

Research output: Contribution to journalArticleResearchpeer-review

13 Citations (Scopus)

Abstract

To gain insight into the adrenal stress response, we analysed differential mRNA expression of genes associated with psychosocial stress in the pig (Sus scrofa domestica). Various levels of psychosocial stress were induced by mixing groups of unfamiliar pigs with different aggressiveness. We selected two experimental groups for comparison, each comprising eight animals, which differed significantly in aggressive behaviour and plasma cortisol levels. To identify differentially expressed genes, we compared the adrenal transcriptome of these two groups of pigs, using the Affymetrix GeneChip porcine Genome Array. Bioinformatic analysis revealed that psychosocial stress induced upregulation of transcripts enriched for functions associated with cholesterol accumulation and downregulation of transcripts enriched for functions associated with cell growth and death. These responses are similar to those induced by ACTH stimulation. Nevertheless, the majority of the differentially expressed genes were so far not described as ACTH responsive. Some, such as GAL and GALP, may have responded to sympathoadrenal stimulation. Several of the differentially expressed transcripts, such as AGT, are associated with processes modulating steroidogenic response of adrenocortical cells to ACTH. One of the most significant findings was upregulation of LOC100039095, comprising a precursor of the microRNA miR-202, pointing to a previously unrecognised layer of regulation of adrenal steroidogenesis by microRNA. Our study, performed under entirely physiological conditions, complements previous studies focusing either on a single adrenal tissue and/or on a single stimulus, and contributes to understanding of the fine-tuning of adrenal stress response.
Original languageEnglish
Pages (from-to)165 - 174
Number of pages10
JournalJournal of Molecular Endocrinology
Volume46
Publication statusFirst published - 2011

Fingerprint

adrenal glands
corticotropin
gene expression
swine
microRNA
aggression
stress response
steroidogenesis
bioinformatics
transcriptome
cortisol
cell death
cell growth
complement
genes
cholesterol
genome
animals
cells

Bibliographical note

62100072
52140022

Keywords

  • Gene expression
  • Porcine

Cite this

Murani, E ; Ponsuksili, S ; D'Eath, RB ; Turner, SP ; Evans, G ; Thoelking, L ; Kurt, E ; Klont, R ; Foury, A ; Mormede, P ; Wimmers, K. / Differential mRNA expression of genes in the porcine adrenal gland associated with psychosocial stress. In: Journal of Molecular Endocrinology. 2011 ; Vol. 46. pp. 165 - 174.
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abstract = "To gain insight into the adrenal stress response, we analysed differential mRNA expression of genes associated with psychosocial stress in the pig (Sus scrofa domestica). Various levels of psychosocial stress were induced by mixing groups of unfamiliar pigs with different aggressiveness. We selected two experimental groups for comparison, each comprising eight animals, which differed significantly in aggressive behaviour and plasma cortisol levels. To identify differentially expressed genes, we compared the adrenal transcriptome of these two groups of pigs, using the Affymetrix GeneChip porcine Genome Array. Bioinformatic analysis revealed that psychosocial stress induced upregulation of transcripts enriched for functions associated with cholesterol accumulation and downregulation of transcripts enriched for functions associated with cell growth and death. These responses are similar to those induced by ACTH stimulation. Nevertheless, the majority of the differentially expressed genes were so far not described as ACTH responsive. Some, such as GAL and GALP, may have responded to sympathoadrenal stimulation. Several of the differentially expressed transcripts, such as AGT, are associated with processes modulating steroidogenic response of adrenocortical cells to ACTH. One of the most significant findings was upregulation of LOC100039095, comprising a precursor of the microRNA miR-202, pointing to a previously unrecognised layer of regulation of adrenal steroidogenesis by microRNA. Our study, performed under entirely physiological conditions, complements previous studies focusing either on a single adrenal tissue and/or on a single stimulus, and contributes to understanding of the fine-tuning of adrenal stress response.",
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Murani, E, Ponsuksili, S, D'Eath, RB, Turner, SP, Evans, G, Thoelking, L, Kurt, E, Klont, R, Foury, A, Mormede, P & Wimmers, K 2011, 'Differential mRNA expression of genes in the porcine adrenal gland associated with psychosocial stress', Journal of Molecular Endocrinology, vol. 46, pp. 165 - 174.

Differential mRNA expression of genes in the porcine adrenal gland associated with psychosocial stress. / Murani, E; Ponsuksili, S; D'Eath, RB; Turner, SP; Evans, G; Thoelking, L; Kurt, E; Klont, R; Foury, A; Mormede, P; Wimmers, K.

In: Journal of Molecular Endocrinology, Vol. 46, 2011, p. 165 - 174.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Differential mRNA expression of genes in the porcine adrenal gland associated with psychosocial stress

AU - Murani, E

AU - Ponsuksili, S

AU - D'Eath, RB

AU - Turner, SP

AU - Evans, G

AU - Thoelking, L

AU - Kurt, E

AU - Klont, R

AU - Foury, A

AU - Mormede, P

AU - Wimmers, K

N1 - 62100072 52140022

PY - 2011

Y1 - 2011

N2 - To gain insight into the adrenal stress response, we analysed differential mRNA expression of genes associated with psychosocial stress in the pig (Sus scrofa domestica). Various levels of psychosocial stress were induced by mixing groups of unfamiliar pigs with different aggressiveness. We selected two experimental groups for comparison, each comprising eight animals, which differed significantly in aggressive behaviour and plasma cortisol levels. To identify differentially expressed genes, we compared the adrenal transcriptome of these two groups of pigs, using the Affymetrix GeneChip porcine Genome Array. Bioinformatic analysis revealed that psychosocial stress induced upregulation of transcripts enriched for functions associated with cholesterol accumulation and downregulation of transcripts enriched for functions associated with cell growth and death. These responses are similar to those induced by ACTH stimulation. Nevertheless, the majority of the differentially expressed genes were so far not described as ACTH responsive. Some, such as GAL and GALP, may have responded to sympathoadrenal stimulation. Several of the differentially expressed transcripts, such as AGT, are associated with processes modulating steroidogenic response of adrenocortical cells to ACTH. One of the most significant findings was upregulation of LOC100039095, comprising a precursor of the microRNA miR-202, pointing to a previously unrecognised layer of regulation of adrenal steroidogenesis by microRNA. Our study, performed under entirely physiological conditions, complements previous studies focusing either on a single adrenal tissue and/or on a single stimulus, and contributes to understanding of the fine-tuning of adrenal stress response.

AB - To gain insight into the adrenal stress response, we analysed differential mRNA expression of genes associated with psychosocial stress in the pig (Sus scrofa domestica). Various levels of psychosocial stress were induced by mixing groups of unfamiliar pigs with different aggressiveness. We selected two experimental groups for comparison, each comprising eight animals, which differed significantly in aggressive behaviour and plasma cortisol levels. To identify differentially expressed genes, we compared the adrenal transcriptome of these two groups of pigs, using the Affymetrix GeneChip porcine Genome Array. Bioinformatic analysis revealed that psychosocial stress induced upregulation of transcripts enriched for functions associated with cholesterol accumulation and downregulation of transcripts enriched for functions associated with cell growth and death. These responses are similar to those induced by ACTH stimulation. Nevertheless, the majority of the differentially expressed genes were so far not described as ACTH responsive. Some, such as GAL and GALP, may have responded to sympathoadrenal stimulation. Several of the differentially expressed transcripts, such as AGT, are associated with processes modulating steroidogenic response of adrenocortical cells to ACTH. One of the most significant findings was upregulation of LOC100039095, comprising a precursor of the microRNA miR-202, pointing to a previously unrecognised layer of regulation of adrenal steroidogenesis by microRNA. Our study, performed under entirely physiological conditions, complements previous studies focusing either on a single adrenal tissue and/or on a single stimulus, and contributes to understanding of the fine-tuning of adrenal stress response.

KW - Gene expression

KW - Porcine

M3 - Article

VL - 46

SP - 165

EP - 174

JO - Journal of Molecular Endocrinology

JF - Journal of Molecular Endocrinology

SN - 0952-5041

ER -