Diversity and genetic differentiation among subpopulations of Gliricidia sepium revealed by PCR-based assays.

I. K. Dawson; A. J. Simons; R. Waugh; W. Powell

doi:10.1038/hdy.1995.2

Diversity and genetic differentiation among subpopulations of Gliricidia sepium revealed by PCR-based assays.

I. K. Dawson, A. J. Simons, R. Waugh, W. Powell

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114 Citations (Scopus)

Abstract

Randomly amplified polymorphic DNA (RAPD), and a mitochondrial marker based on amplification of the V7 region of the mitochondrial small ribosomal RNA (srRNA) gene, were used to partition genetic variation within a single population of Gliricidia sepium sampled from Guatemala. Seventeen per cent of the variation detected with RAPDs was partitioned among subpopulations and indicated a greater level of discrimination than previously detected with isozymes. Cluster analysis indicated a direct relationship between this variation and the geographical distance between subpopulations. A polymorphism identified within the maternally inherited mitochondrial V7 srRNA product, which relied on digestion with restriction endonucleases, confirmed the genetic subdivision identified with RAPDs, and suggested a relatively limited role for seed in gene dispersal.

Original language	English
Pages (from-to)	10-18
Number of pages	9
Journal	Heredity
Volume	74
Issue number	1
DOIs	https://doi.org/10.1038/hdy.1995.2 https://doi.org/10.1038/hdy.1995.2
Publication status	Print publication - 1995
Externally published	Yes

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title = "Diversity and genetic differentiation among subpopulations of Gliricidia sepium revealed by PCR-based assays.",

abstract = "Randomly amplified polymorphic DNA (RAPD), and a mitochondrial marker based on amplification of the V7 region of the mitochondrial small ribosomal RNA (srRNA) gene, were used to partition genetic variation within a single population of Gliricidia sepium sampled from Guatemala. Seventeen per cent of the variation detected with RAPDs was partitioned among subpopulations and indicated a greater level of discrimination than previously detected with isozymes. Cluster analysis indicated a direct relationship between this variation and the geographical distance between subpopulations. A polymorphism identified within the maternally inherited mitochondrial V7 srRNA product, which relied on digestion with restriction endonucleases, confirmed the genetic subdivision identified with RAPDs, and suggested a relatively limited role for seed in gene dispersal.",

author = "Dawson, {I. K.} and Simons, {A. J.} and R. Waugh and W. Powell",

year = "1995",

doi = "10.1038/hdy.1995.2",

language = "English",

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pages = "10--18",

journal = "Heredity",

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T1 - Diversity and genetic differentiation among subpopulations of Gliricidia sepium revealed by PCR-based assays.

AU - Dawson, I. K.

AU - Simons, A. J.

AU - Waugh, R.

AU - Powell, W.

PY - 1995

Y1 - 1995

N2 - Randomly amplified polymorphic DNA (RAPD), and a mitochondrial marker based on amplification of the V7 region of the mitochondrial small ribosomal RNA (srRNA) gene, were used to partition genetic variation within a single population of Gliricidia sepium sampled from Guatemala. Seventeen per cent of the variation detected with RAPDs was partitioned among subpopulations and indicated a greater level of discrimination than previously detected with isozymes. Cluster analysis indicated a direct relationship between this variation and the geographical distance between subpopulations. A polymorphism identified within the maternally inherited mitochondrial V7 srRNA product, which relied on digestion with restriction endonucleases, confirmed the genetic subdivision identified with RAPDs, and suggested a relatively limited role for seed in gene dispersal.

AB - Randomly amplified polymorphic DNA (RAPD), and a mitochondrial marker based on amplification of the V7 region of the mitochondrial small ribosomal RNA (srRNA) gene, were used to partition genetic variation within a single population of Gliricidia sepium sampled from Guatemala. Seventeen per cent of the variation detected with RAPDs was partitioned among subpopulations and indicated a greater level of discrimination than previously detected with isozymes. Cluster analysis indicated a direct relationship between this variation and the geographical distance between subpopulations. A polymorphism identified within the maternally inherited mitochondrial V7 srRNA product, which relied on digestion with restriction endonucleases, confirmed the genetic subdivision identified with RAPDs, and suggested a relatively limited role for seed in gene dispersal.

UR - http://www.mendeley.com/research/diversity-genetic-differentiation-among-subpopulations-guricidia-sepium-revealed-pcrbased-assays

U2 - 10.1038/hdy.1995.2

DO - 10.1038/hdy.1995.2

M3 - Article

SN - 0018-067X

VL - 74

SP - 10

EP - 18

JO - Heredity

JF - Heredity

IS - 1

ER -

Diversity and genetic differentiation among subpopulations of Gliricidia sepium revealed by PCR-based assays.

Abstract

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