Duodenal flow and digestibility in fauna-free sheep and in sheep monofaunated with Entodinium caudatum or Polyplastron multivesiculatum

M Ivan, K M Koenig, D P Morgavi, L M Rode, C J Newbold, T Entz

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Three groups of five rumen and duodenum cannulated fauna-free sheep were used in a 28 d experiment. One group remained fauna-free, whereas the second (EN) and third (PP) groups, respectively, were inoculated intraruminally with the protozoan species Entodinium caudatum and Polyplastron multivesiculatum. Rumen fluid, duodenal digesta and faecal samples were collected during the last 12 d. The flow of digesta to the duodenum was determined using Yb and Co as dual-phase markers. (15)Nitrogen and phosphatidylcholine were used as markers to calculate the duodenal flow of bacterial and protozoal N, respectively. Results showed an increase (P < 0.1) in the rumen concentration of NH3-N and total volatile fatty acids, and a decrease (P < 0.05) in the duodenal flow of non-NH3-N and bacterial N in sheep with EN and PP monofaunas, compared with fauna-free sheep. There were no differences (P > 0.05) in these variables between the two monofauna groups. Protozoal N accounted for 8 % of the duodenal non-NH3-N flow in the EN-monofaunated sheep, whereas no such flow was detected in the PP-monofaunated sheep. Apparent rumen digestibility of organic matter, neutral detergent fibre and acid detergent fibre were similar (P > 0.05) in the monofaunated groups of sheep, but rumen acid detergent fibre digestibility was higher (P < 0.05) in the monofaunated than in the fauna-free groups. Experimental results suggested that, unlike EN, the PP monofauna might not contribute to the duodenal flow of microbial protein, whereas both monofaunas showed a virtually equal degree of predation on rumen bacteria.

Original languageEnglish
Pages (from-to)469-76
Number of pages8
JournalBritish Journal of Nutrition
Volume95
Issue number3
Publication statusPrint publication - Mar 2006
Externally publishedYes

Fingerprint

Urodela
Rumen
Sheep
Detergents
Duodenum
Acids
Phosphatidylcholines
Nitrogen
Bacteria
Proteins

Keywords

  • Animal Feed
  • Animals
  • Biomarkers/analysis
  • Ciliophora/isolation & purification
  • Dietary Fiber/metabolism
  • Digestion/physiology
  • Duodenum/parasitology
  • Fermentation/physiology
  • Male
  • Nitrates/metabolism
  • Nitrogen/administration & dosage
  • Phosphatidylcholines/analysis
  • Rumen/parasitology
  • Sheep

Cite this

@article{8381c127d4454e0496d603b33667ee89,
title = "Duodenal flow and digestibility in fauna-free sheep and in sheep monofaunated with Entodinium caudatum or Polyplastron multivesiculatum",
abstract = "Three groups of five rumen and duodenum cannulated fauna-free sheep were used in a 28 d experiment. One group remained fauna-free, whereas the second (EN) and third (PP) groups, respectively, were inoculated intraruminally with the protozoan species Entodinium caudatum and Polyplastron multivesiculatum. Rumen fluid, duodenal digesta and faecal samples were collected during the last 12 d. The flow of digesta to the duodenum was determined using Yb and Co as dual-phase markers. (15)Nitrogen and phosphatidylcholine were used as markers to calculate the duodenal flow of bacterial and protozoal N, respectively. Results showed an increase (P < 0.1) in the rumen concentration of NH3-N and total volatile fatty acids, and a decrease (P < 0.05) in the duodenal flow of non-NH3-N and bacterial N in sheep with EN and PP monofaunas, compared with fauna-free sheep. There were no differences (P > 0.05) in these variables between the two monofauna groups. Protozoal N accounted for 8 {\%} of the duodenal non-NH3-N flow in the EN-monofaunated sheep, whereas no such flow was detected in the PP-monofaunated sheep. Apparent rumen digestibility of organic matter, neutral detergent fibre and acid detergent fibre were similar (P > 0.05) in the monofaunated groups of sheep, but rumen acid detergent fibre digestibility was higher (P < 0.05) in the monofaunated than in the fauna-free groups. Experimental results suggested that, unlike EN, the PP monofauna might not contribute to the duodenal flow of microbial protein, whereas both monofaunas showed a virtually equal degree of predation on rumen bacteria.",
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author = "M Ivan and Koenig, {K M} and Morgavi, {D P} and Rode, {L M} and Newbold, {C J} and T Entz",
year = "2006",
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pages = "469--76",
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Duodenal flow and digestibility in fauna-free sheep and in sheep monofaunated with Entodinium caudatum or Polyplastron multivesiculatum. / Ivan, M; Koenig, K M; Morgavi, D P; Rode, L M; Newbold, C J; Entz, T.

In: British Journal of Nutrition, Vol. 95, No. 3, 03.2006, p. 469-76.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Duodenal flow and digestibility in fauna-free sheep and in sheep monofaunated with Entodinium caudatum or Polyplastron multivesiculatum

AU - Ivan, M

AU - Koenig, K M

AU - Morgavi, D P

AU - Rode, L M

AU - Newbold, C J

AU - Entz, T

PY - 2006/3

Y1 - 2006/3

N2 - Three groups of five rumen and duodenum cannulated fauna-free sheep were used in a 28 d experiment. One group remained fauna-free, whereas the second (EN) and third (PP) groups, respectively, were inoculated intraruminally with the protozoan species Entodinium caudatum and Polyplastron multivesiculatum. Rumen fluid, duodenal digesta and faecal samples were collected during the last 12 d. The flow of digesta to the duodenum was determined using Yb and Co as dual-phase markers. (15)Nitrogen and phosphatidylcholine were used as markers to calculate the duodenal flow of bacterial and protozoal N, respectively. Results showed an increase (P < 0.1) in the rumen concentration of NH3-N and total volatile fatty acids, and a decrease (P < 0.05) in the duodenal flow of non-NH3-N and bacterial N in sheep with EN and PP monofaunas, compared with fauna-free sheep. There were no differences (P > 0.05) in these variables between the two monofauna groups. Protozoal N accounted for 8 % of the duodenal non-NH3-N flow in the EN-monofaunated sheep, whereas no such flow was detected in the PP-monofaunated sheep. Apparent rumen digestibility of organic matter, neutral detergent fibre and acid detergent fibre were similar (P > 0.05) in the monofaunated groups of sheep, but rumen acid detergent fibre digestibility was higher (P < 0.05) in the monofaunated than in the fauna-free groups. Experimental results suggested that, unlike EN, the PP monofauna might not contribute to the duodenal flow of microbial protein, whereas both monofaunas showed a virtually equal degree of predation on rumen bacteria.

AB - Three groups of five rumen and duodenum cannulated fauna-free sheep were used in a 28 d experiment. One group remained fauna-free, whereas the second (EN) and third (PP) groups, respectively, were inoculated intraruminally with the protozoan species Entodinium caudatum and Polyplastron multivesiculatum. Rumen fluid, duodenal digesta and faecal samples were collected during the last 12 d. The flow of digesta to the duodenum was determined using Yb and Co as dual-phase markers. (15)Nitrogen and phosphatidylcholine were used as markers to calculate the duodenal flow of bacterial and protozoal N, respectively. Results showed an increase (P < 0.1) in the rumen concentration of NH3-N and total volatile fatty acids, and a decrease (P < 0.05) in the duodenal flow of non-NH3-N and bacterial N in sheep with EN and PP monofaunas, compared with fauna-free sheep. There were no differences (P > 0.05) in these variables between the two monofauna groups. Protozoal N accounted for 8 % of the duodenal non-NH3-N flow in the EN-monofaunated sheep, whereas no such flow was detected in the PP-monofaunated sheep. Apparent rumen digestibility of organic matter, neutral detergent fibre and acid detergent fibre were similar (P > 0.05) in the monofaunated groups of sheep, but rumen acid detergent fibre digestibility was higher (P < 0.05) in the monofaunated than in the fauna-free groups. Experimental results suggested that, unlike EN, the PP monofauna might not contribute to the duodenal flow of microbial protein, whereas both monofaunas showed a virtually equal degree of predation on rumen bacteria.

KW - Animal Feed

KW - Animals

KW - Biomarkers/analysis

KW - Ciliophora/isolation & purification

KW - Dietary Fiber/metabolism

KW - Digestion/physiology

KW - Duodenum/parasitology

KW - Fermentation/physiology

KW - Male

KW - Nitrates/metabolism

KW - Nitrogen/administration & dosage

KW - Phosphatidylcholines/analysis

KW - Rumen/parasitology

KW - Sheep

M3 - Article

C2 - 16512932

VL - 95

SP - 469

EP - 476

JO - British Journal of Nutrition

JF - British Journal of Nutrition

SN - 0007-1145

IS - 3

ER -