Early postnatal immunisation against gonadotrophin-releasing hormone induces a high but differential immune response in heifer calves

JH Hernandez-Medrano, RW Williams, S van Drunen Littel-van den Hurk, AR Peters, D Hannant, BK Campbell, R Webb

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The aim of this study was to evaluate endocrinological and immunological effects of early postnatal immunisation against gonadotrophin-releasing hormone (GnRH) in heifer calves, as similar treatment in sheep provokes long-term immunocastration. Heifer calves were injected with either a construct of GnRH – bovine herpes virus 1 glycoprotein D (BHV1 gD; n = 9) or saline (n = 9) at 2, 6 and 13.5 weeks of age. Antibody (GnRH and carrier) and endocrine responses to immunisation were measured twice monthly (FSH and progesterone) or during intensive sampling regimes (LH). Early postnatal immunisation against GnRH induced a high, but variable, antibody response against both GnRH and carrier. Based on antibody responses, animals were divided into high-titre (HT, n = 5) and low-titre (LT, n = 4). Occurring mainly in HT, a further peak in anti-GnRH antibodies, stimulated independently of the carrier, was observed at 23 weeks of age, with antibody titres ⩾10% binding for ∼9 weeks post-peak. Conversely immunisation had only temporary, reversible effects on reproductive function, not affecting age at puberty. We hypothesise that the newly generated antibody measured 10 weeks after the final immunisation resulted from antigenic stimulation and immunological memory cell activation to an endogenous GnRH release. This outcome offers an opportunity for further manipulation of reproductive function based on modulation of GnRH secretion and activity where long-term immunological memory may contribute to durable endocrine effects.
Original languageEnglish
Pages (from-to)472 - 479
Number of pages8
JournalResearch in Veterinary Science
Volume95
Issue number2
Early online date15 Jun 2013
DOIs
Publication statusPrint publication - Oct 2013

Fingerprint

Gonadotropin-Releasing Hormone
Immunization
Immunologic Memory
Antibodies
Antibody Formation
Long-Term Memory
Puberty
Progesterone
Serotonin
Sheep
Glycoproteins
Viruses

Keywords

  • GnRH immunisation
  • Immunocastration
  • Immunosterilisation
  • Neonatal immunisation

Cite this

Hernandez-Medrano, JH., Williams, RW., van Drunen Littel-van den Hurk, S., Peters, AR., Hannant, D., Campbell, BK., & Webb, R. (2013). Early postnatal immunisation against gonadotrophin-releasing hormone induces a high but differential immune response in heifer calves. Research in Veterinary Science, 95(2), 472 - 479. https://doi.org/10.1016/j.rvsc.2013.05.014
Hernandez-Medrano, JH ; Williams, RW ; van Drunen Littel-van den Hurk, S ; Peters, AR ; Hannant, D ; Campbell, BK ; Webb, R. / Early postnatal immunisation against gonadotrophin-releasing hormone induces a high but differential immune response in heifer calves. In: Research in Veterinary Science. 2013 ; Vol. 95, No. 2. pp. 472 - 479.
@article{b8f4223905404847ac97e495869f65f9,
title = "Early postnatal immunisation against gonadotrophin-releasing hormone induces a high but differential immune response in heifer calves",
abstract = "The aim of this study was to evaluate endocrinological and immunological effects of early postnatal immunisation against gonadotrophin-releasing hormone (GnRH) in heifer calves, as similar treatment in sheep provokes long-term immunocastration. Heifer calves were injected with either a construct of GnRH – bovine herpes virus 1 glycoprotein D (BHV1 gD; n = 9) or saline (n = 9) at 2, 6 and 13.5 weeks of age. Antibody (GnRH and carrier) and endocrine responses to immunisation were measured twice monthly (FSH and progesterone) or during intensive sampling regimes (LH). Early postnatal immunisation against GnRH induced a high, but variable, antibody response against both GnRH and carrier. Based on antibody responses, animals were divided into high-titre (HT, n = 5) and low-titre (LT, n = 4). Occurring mainly in HT, a further peak in anti-GnRH antibodies, stimulated independently of the carrier, was observed at 23 weeks of age, with antibody titres ⩾10{\%} binding for ∼9 weeks post-peak. Conversely immunisation had only temporary, reversible effects on reproductive function, not affecting age at puberty. We hypothesise that the newly generated antibody measured 10 weeks after the final immunisation resulted from antigenic stimulation and immunological memory cell activation to an endogenous GnRH release. This outcome offers an opportunity for further manipulation of reproductive function based on modulation of GnRH secretion and activity where long-term immunological memory may contribute to durable endocrine effects.",
keywords = "GnRH immunisation, Immunocastration, Immunosterilisation, Neonatal immunisation",
author = "JH Hernandez-Medrano and RW Williams and {van Drunen Littel-van den Hurk}, S and AR Peters and D Hannant and BK Campbell and R Webb",
year = "2013",
month = "10",
doi = "10.1016/j.rvsc.2013.05.014",
language = "English",
volume = "95",
pages = "472 -- 479",
journal = "Research in Veterinary Science",
issn = "0034-5288",
publisher = "Elsevier",
number = "2",

}

Hernandez-Medrano, JH, Williams, RW, van Drunen Littel-van den Hurk, S, Peters, AR, Hannant, D, Campbell, BK & Webb, R 2013, 'Early postnatal immunisation against gonadotrophin-releasing hormone induces a high but differential immune response in heifer calves', Research in Veterinary Science, vol. 95, no. 2, pp. 472 - 479. https://doi.org/10.1016/j.rvsc.2013.05.014

Early postnatal immunisation against gonadotrophin-releasing hormone induces a high but differential immune response in heifer calves. / Hernandez-Medrano, JH; Williams, RW; van Drunen Littel-van den Hurk, S; Peters, AR; Hannant, D; Campbell, BK; Webb, R.

In: Research in Veterinary Science, Vol. 95, No. 2, 10.2013, p. 472 - 479.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Early postnatal immunisation against gonadotrophin-releasing hormone induces a high but differential immune response in heifer calves

AU - Hernandez-Medrano, JH

AU - Williams, RW

AU - van Drunen Littel-van den Hurk, S

AU - Peters, AR

AU - Hannant, D

AU - Campbell, BK

AU - Webb, R

PY - 2013/10

Y1 - 2013/10

N2 - The aim of this study was to evaluate endocrinological and immunological effects of early postnatal immunisation against gonadotrophin-releasing hormone (GnRH) in heifer calves, as similar treatment in sheep provokes long-term immunocastration. Heifer calves were injected with either a construct of GnRH – bovine herpes virus 1 glycoprotein D (BHV1 gD; n = 9) or saline (n = 9) at 2, 6 and 13.5 weeks of age. Antibody (GnRH and carrier) and endocrine responses to immunisation were measured twice monthly (FSH and progesterone) or during intensive sampling regimes (LH). Early postnatal immunisation against GnRH induced a high, but variable, antibody response against both GnRH and carrier. Based on antibody responses, animals were divided into high-titre (HT, n = 5) and low-titre (LT, n = 4). Occurring mainly in HT, a further peak in anti-GnRH antibodies, stimulated independently of the carrier, was observed at 23 weeks of age, with antibody titres ⩾10% binding for ∼9 weeks post-peak. Conversely immunisation had only temporary, reversible effects on reproductive function, not affecting age at puberty. We hypothesise that the newly generated antibody measured 10 weeks after the final immunisation resulted from antigenic stimulation and immunological memory cell activation to an endogenous GnRH release. This outcome offers an opportunity for further manipulation of reproductive function based on modulation of GnRH secretion and activity where long-term immunological memory may contribute to durable endocrine effects.

AB - The aim of this study was to evaluate endocrinological and immunological effects of early postnatal immunisation against gonadotrophin-releasing hormone (GnRH) in heifer calves, as similar treatment in sheep provokes long-term immunocastration. Heifer calves were injected with either a construct of GnRH – bovine herpes virus 1 glycoprotein D (BHV1 gD; n = 9) or saline (n = 9) at 2, 6 and 13.5 weeks of age. Antibody (GnRH and carrier) and endocrine responses to immunisation were measured twice monthly (FSH and progesterone) or during intensive sampling regimes (LH). Early postnatal immunisation against GnRH induced a high, but variable, antibody response against both GnRH and carrier. Based on antibody responses, animals were divided into high-titre (HT, n = 5) and low-titre (LT, n = 4). Occurring mainly in HT, a further peak in anti-GnRH antibodies, stimulated independently of the carrier, was observed at 23 weeks of age, with antibody titres ⩾10% binding for ∼9 weeks post-peak. Conversely immunisation had only temporary, reversible effects on reproductive function, not affecting age at puberty. We hypothesise that the newly generated antibody measured 10 weeks after the final immunisation resulted from antigenic stimulation and immunological memory cell activation to an endogenous GnRH release. This outcome offers an opportunity for further manipulation of reproductive function based on modulation of GnRH secretion and activity where long-term immunological memory may contribute to durable endocrine effects.

KW - GnRH immunisation

KW - Immunocastration

KW - Immunosterilisation

KW - Neonatal immunisation

U2 - 10.1016/j.rvsc.2013.05.014

DO - 10.1016/j.rvsc.2013.05.014

M3 - Article

VL - 95

SP - 472

EP - 479

JO - Research in Veterinary Science

JF - Research in Veterinary Science

SN - 0034-5288

IS - 2

ER -

Hernandez-Medrano JH, Williams RW, van Drunen Littel-van den Hurk S, Peters AR, Hannant D, Campbell BK et al. Early postnatal immunisation against gonadotrophin-releasing hormone induces a high but differential immune response in heifer calves. Research in Veterinary Science. 2013 Oct;95(2):472 - 479. https://doi.org/10.1016/j.rvsc.2013.05.014