Effect of bromochloromethane on methane emission, rumen fermentation pattern, milk yield, and fatty acid profile in lactating dairy goats

L Abecia, P G Toral, A I Martín-García, G Martínez, N W Tomkins, E Molina-Alcaide, C J Newbold, D R Yáñez-Ruiz

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

Several technologies have been tested to reduce enteric methanogenesis, but very few have been successfully used in practical conditions for livestock. Furthermore, the consequences of reduced rumen methane production on animal performance and milk quality are poorly understood. The aim of this work was to investigate the effect of feeding bromochloromethane (BCM), a halogenated aliphatic hydrocarbon with potential antimethanogenic activity, to dairy goats on rumen methane production, fermentation pattern, the abundance of major microbial groups, and on animal performance and milk composition. Eighteen goats were allocated to 2 experimental groups of 9 animals each: treated (BCM+) or not (BCM-) with 0.30 g of BCM/100 kg of body weight per day. The BCM was administered per os in 2 equal doses per day from parturition to 2 wk postweaning (10 wk). After weaning, methane emissions were recorded over 2 consecutive days (d 57 and 58 on treatment) in polycarbonate chambers. On d 59, individual rumen fluid samples were collected for volatile fatty acid (VFA) analysis and quantification of bacterial, protozoal, and archaeal numbers by real-time PCR. On d 69 and 70, daily milk production was recorded and samples were collected for determination of fat, protein, lactose, casein, and total solids concentration by infrared spectrophotometry, and fatty acid composition by gas chromatography. Treatment with BCM reduced methane production by 33% (21.6 vs. 14.4 L/kg of DMI) compared with nontreated animals, although it did not affect the abundance of rumen bacteria, protozoa, and total methanogenic archaea. The observed improvement in the efficiency of digestive processes was accompanied by a 36% increase in milk yield, probably due to the more propionic type of rumen fermentation and an increase in VFA production. The increase in milk yield was not accompanied by any changes in the concentrations or yields of fat, protein, or lactose. Despite the substantial decrease in methane production, only minor changes in milk fatty acid profile were observed, suggesting that ruminal biohydrogenation pathways were not affected. Compounds that influence terminal biochemical pathways for methane production deserve further development for future application in the dairy goat sector.

Original languageEnglish
Pages (from-to)2027-36
Number of pages10
JournalJournal of Dairy Science
Volume95
Issue number4
DOIs
Publication statusPrint publication - Apr 2012
Externally publishedYes

Fingerprint

dairy goats
Rumen
Methane
methane production
rumen fermentation
Goats
methane
Fermentation
milk yield
Milk
Fatty Acids
fatty acid composition
maternal milk
polycarbonate
Volatile Fatty Acids
animal performance
Lactose
volatile fatty acids
lactose
rumen

Bibliographical note

Copyright © 2012 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

Keywords

  • Animals
  • Diet/veterinary
  • Fatty Acids/analysis
  • Fatty Acids, Volatile/analysis
  • Female
  • Fermentation/drug effects
  • Goats/metabolism
  • Hydrocarbons, Halogenated/administration & dosage
  • Lactation/drug effects
  • Methane/biosynthesis
  • Milk/chemistry
  • Rumen/drug effects

Cite this

Abecia, L., Toral, P. G., Martín-García, A. I., Martínez, G., Tomkins, N. W., Molina-Alcaide, E., ... Yáñez-Ruiz, D. R. (2012). Effect of bromochloromethane on methane emission, rumen fermentation pattern, milk yield, and fatty acid profile in lactating dairy goats. Journal of Dairy Science, 95(4), 2027-36. https://doi.org/10.3168/jds.2011-4831
Abecia, L ; Toral, P G ; Martín-García, A I ; Martínez, G ; Tomkins, N W ; Molina-Alcaide, E ; Newbold, C J ; Yáñez-Ruiz, D R. / Effect of bromochloromethane on methane emission, rumen fermentation pattern, milk yield, and fatty acid profile in lactating dairy goats. In: Journal of Dairy Science. 2012 ; Vol. 95, No. 4. pp. 2027-36.
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abstract = "Several technologies have been tested to reduce enteric methanogenesis, but very few have been successfully used in practical conditions for livestock. Furthermore, the consequences of reduced rumen methane production on animal performance and milk quality are poorly understood. The aim of this work was to investigate the effect of feeding bromochloromethane (BCM), a halogenated aliphatic hydrocarbon with potential antimethanogenic activity, to dairy goats on rumen methane production, fermentation pattern, the abundance of major microbial groups, and on animal performance and milk composition. Eighteen goats were allocated to 2 experimental groups of 9 animals each: treated (BCM+) or not (BCM-) with 0.30 g of BCM/100 kg of body weight per day. The BCM was administered per os in 2 equal doses per day from parturition to 2 wk postweaning (10 wk). After weaning, methane emissions were recorded over 2 consecutive days (d 57 and 58 on treatment) in polycarbonate chambers. On d 59, individual rumen fluid samples were collected for volatile fatty acid (VFA) analysis and quantification of bacterial, protozoal, and archaeal numbers by real-time PCR. On d 69 and 70, daily milk production was recorded and samples were collected for determination of fat, protein, lactose, casein, and total solids concentration by infrared spectrophotometry, and fatty acid composition by gas chromatography. Treatment with BCM reduced methane production by 33{\%} (21.6 vs. 14.4 L/kg of DMI) compared with nontreated animals, although it did not affect the abundance of rumen bacteria, protozoa, and total methanogenic archaea. The observed improvement in the efficiency of digestive processes was accompanied by a 36{\%} increase in milk yield, probably due to the more propionic type of rumen fermentation and an increase in VFA production. The increase in milk yield was not accompanied by any changes in the concentrations or yields of fat, protein, or lactose. Despite the substantial decrease in methane production, only minor changes in milk fatty acid profile were observed, suggesting that ruminal biohydrogenation pathways were not affected. Compounds that influence terminal biochemical pathways for methane production deserve further development for future application in the dairy goat sector.",
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Abecia, L, Toral, PG, Martín-García, AI, Martínez, G, Tomkins, NW, Molina-Alcaide, E, Newbold, CJ & Yáñez-Ruiz, DR 2012, 'Effect of bromochloromethane on methane emission, rumen fermentation pattern, milk yield, and fatty acid profile in lactating dairy goats', Journal of Dairy Science, vol. 95, no. 4, pp. 2027-36. https://doi.org/10.3168/jds.2011-4831

Effect of bromochloromethane on methane emission, rumen fermentation pattern, milk yield, and fatty acid profile in lactating dairy goats. / Abecia, L; Toral, P G; Martín-García, A I; Martínez, G; Tomkins, N W; Molina-Alcaide, E; Newbold, C J; Yáñez-Ruiz, D R.

In: Journal of Dairy Science, Vol. 95, No. 4, 04.2012, p. 2027-36.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effect of bromochloromethane on methane emission, rumen fermentation pattern, milk yield, and fatty acid profile in lactating dairy goats

AU - Abecia, L

AU - Toral, P G

AU - Martín-García, A I

AU - Martínez, G

AU - Tomkins, N W

AU - Molina-Alcaide, E

AU - Newbold, C J

AU - Yáñez-Ruiz, D R

N1 - Copyright © 2012 American Dairy Science Association. Published by Elsevier Inc. All rights reserved.

PY - 2012/4

Y1 - 2012/4

N2 - Several technologies have been tested to reduce enteric methanogenesis, but very few have been successfully used in practical conditions for livestock. Furthermore, the consequences of reduced rumen methane production on animal performance and milk quality are poorly understood. The aim of this work was to investigate the effect of feeding bromochloromethane (BCM), a halogenated aliphatic hydrocarbon with potential antimethanogenic activity, to dairy goats on rumen methane production, fermentation pattern, the abundance of major microbial groups, and on animal performance and milk composition. Eighteen goats were allocated to 2 experimental groups of 9 animals each: treated (BCM+) or not (BCM-) with 0.30 g of BCM/100 kg of body weight per day. The BCM was administered per os in 2 equal doses per day from parturition to 2 wk postweaning (10 wk). After weaning, methane emissions were recorded over 2 consecutive days (d 57 and 58 on treatment) in polycarbonate chambers. On d 59, individual rumen fluid samples were collected for volatile fatty acid (VFA) analysis and quantification of bacterial, protozoal, and archaeal numbers by real-time PCR. On d 69 and 70, daily milk production was recorded and samples were collected for determination of fat, protein, lactose, casein, and total solids concentration by infrared spectrophotometry, and fatty acid composition by gas chromatography. Treatment with BCM reduced methane production by 33% (21.6 vs. 14.4 L/kg of DMI) compared with nontreated animals, although it did not affect the abundance of rumen bacteria, protozoa, and total methanogenic archaea. The observed improvement in the efficiency of digestive processes was accompanied by a 36% increase in milk yield, probably due to the more propionic type of rumen fermentation and an increase in VFA production. The increase in milk yield was not accompanied by any changes in the concentrations or yields of fat, protein, or lactose. Despite the substantial decrease in methane production, only minor changes in milk fatty acid profile were observed, suggesting that ruminal biohydrogenation pathways were not affected. Compounds that influence terminal biochemical pathways for methane production deserve further development for future application in the dairy goat sector.

AB - Several technologies have been tested to reduce enteric methanogenesis, but very few have been successfully used in practical conditions for livestock. Furthermore, the consequences of reduced rumen methane production on animal performance and milk quality are poorly understood. The aim of this work was to investigate the effect of feeding bromochloromethane (BCM), a halogenated aliphatic hydrocarbon with potential antimethanogenic activity, to dairy goats on rumen methane production, fermentation pattern, the abundance of major microbial groups, and on animal performance and milk composition. Eighteen goats were allocated to 2 experimental groups of 9 animals each: treated (BCM+) or not (BCM-) with 0.30 g of BCM/100 kg of body weight per day. The BCM was administered per os in 2 equal doses per day from parturition to 2 wk postweaning (10 wk). After weaning, methane emissions were recorded over 2 consecutive days (d 57 and 58 on treatment) in polycarbonate chambers. On d 59, individual rumen fluid samples were collected for volatile fatty acid (VFA) analysis and quantification of bacterial, protozoal, and archaeal numbers by real-time PCR. On d 69 and 70, daily milk production was recorded and samples were collected for determination of fat, protein, lactose, casein, and total solids concentration by infrared spectrophotometry, and fatty acid composition by gas chromatography. Treatment with BCM reduced methane production by 33% (21.6 vs. 14.4 L/kg of DMI) compared with nontreated animals, although it did not affect the abundance of rumen bacteria, protozoa, and total methanogenic archaea. The observed improvement in the efficiency of digestive processes was accompanied by a 36% increase in milk yield, probably due to the more propionic type of rumen fermentation and an increase in VFA production. The increase in milk yield was not accompanied by any changes in the concentrations or yields of fat, protein, or lactose. Despite the substantial decrease in methane production, only minor changes in milk fatty acid profile were observed, suggesting that ruminal biohydrogenation pathways were not affected. Compounds that influence terminal biochemical pathways for methane production deserve further development for future application in the dairy goat sector.

KW - Animals

KW - Diet/veterinary

KW - Fatty Acids/analysis

KW - Fatty Acids, Volatile/analysis

KW - Female

KW - Fermentation/drug effects

KW - Goats/metabolism

KW - Hydrocarbons, Halogenated/administration & dosage

KW - Lactation/drug effects

KW - Methane/biosynthesis

KW - Milk/chemistry

KW - Rumen/drug effects

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C2 - 22459848

VL - 95

SP - 2027

EP - 2036

JO - Journal of Dairy Science

JF - Journal of Dairy Science

SN - 0022-0302

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