Effect of dietary Enterolobium cyclocarpum on microbial protein flow and nutrient digestibility in sheep maintained fauna-free, with total mixed fauna or with Entodinium caudatum monofauna

K M Koenig, M Ivan, B T Teferedegne, D P Morgavi, L M Rode, I M Ibrahim, C J Newbold

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Three groups of five wethers with ruminal and duodenal cannulas and maintained as either fauna-free (FF) or inoculated with total mixed fauna (TF) or Entodinium caudatum as a single-species monofauna (EN) were used in an experiment with two 28 d periods. In the first period, the sheep were fed a control barley-based diet (40:60 concentrate to silage DM) and in the second period the diet was supplemented with 187 g DM of Enterolobium cyclocarpum for the last 12 d of the period. The diets of period 1 and 2 were isonitrogenous. There was no effect of fauna on apparent ruminal and total tract organic matter and fibre digestion, but bacterial and microbial N flow and efficiency were improved in FF sheep compared to TF sheep. In period 2, protozoal numbers were reduced between 31 and 88 % 2 h after feeding E. cyclocarpum for the third to twelfth day of supplementation and by an average of 25 % in samples collected over the 24 h feeding cycle. Supplementation of the diet with E. cyclocarpum and the consequent protozoal reduction in TF and EN sheep improved the flow of non-ammonia N and bacterial N to the small intestine and the efficiency of microbial synthesis. However, E. cyclocarpum reduced ruminal organic matter digestion, especially in faunated sheep, and total tract organic matter, N and fibre digestion. Thus, a reduction in the protozoal cell numbers of 25 % was sufficient to achieve the beneficial effects of reduced fauna on the bacterial protein supply, but diet digestibility was reduced.

Original languageEnglish
Pages (from-to)504-16
Number of pages13
JournalBritish Journal of Nutrition
Volume98
Issue number3
DOIs
Publication statusPrint publication - Sep 2007
Externally publishedYes

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Urodela
Sheep
Diet
Food
Digestion
Proteins
Silage
Bacterial Proteins
Hordeum
Small Intestine
Cell Count

Keywords

  • Animal Feed
  • Animals
  • Bacterial Proteins/metabolism
  • Ciliophora/isolation & purification
  • Dietary Fiber/metabolism
  • Dietary Proteins/metabolism
  • Dietary Supplements
  • Digestion/physiology
  • Duodenum/physiology
  • Fabaceae/physiology
  • Fatty Acids, Volatile/analysis
  • Fermentation/physiology
  • Gastrointestinal Transit/physiology
  • Nitrogen/metabolism
  • Protozoan Proteins/metabolism
  • Rumen/metabolism
  • Sheep

Cite this

@article{c88ee7ec465342f0a99faad8f85474a4,
title = "Effect of dietary Enterolobium cyclocarpum on microbial protein flow and nutrient digestibility in sheep maintained fauna-free, with total mixed fauna or with Entodinium caudatum monofauna",
abstract = "Three groups of five wethers with ruminal and duodenal cannulas and maintained as either fauna-free (FF) or inoculated with total mixed fauna (TF) or Entodinium caudatum as a single-species monofauna (EN) were used in an experiment with two 28 d periods. In the first period, the sheep were fed a control barley-based diet (40:60 concentrate to silage DM) and in the second period the diet was supplemented with 187 g DM of Enterolobium cyclocarpum for the last 12 d of the period. The diets of period 1 and 2 were isonitrogenous. There was no effect of fauna on apparent ruminal and total tract organic matter and fibre digestion, but bacterial and microbial N flow and efficiency were improved in FF sheep compared to TF sheep. In period 2, protozoal numbers were reduced between 31 and 88 {\%} 2 h after feeding E. cyclocarpum for the third to twelfth day of supplementation and by an average of 25 {\%} in samples collected over the 24 h feeding cycle. Supplementation of the diet with E. cyclocarpum and the consequent protozoal reduction in TF and EN sheep improved the flow of non-ammonia N and bacterial N to the small intestine and the efficiency of microbial synthesis. However, E. cyclocarpum reduced ruminal organic matter digestion, especially in faunated sheep, and total tract organic matter, N and fibre digestion. Thus, a reduction in the protozoal cell numbers of 25 {\%} was sufficient to achieve the beneficial effects of reduced fauna on the bacterial protein supply, but diet digestibility was reduced.",
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author = "Koenig, {K M} and M Ivan and Teferedegne, {B T} and Morgavi, {D P} and Rode, {L M} and Ibrahim, {I M} and Newbold, {C J}",
year = "2007",
month = "9",
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language = "English",
volume = "98",
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journal = "British Journal of Nutrition",
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Effect of dietary Enterolobium cyclocarpum on microbial protein flow and nutrient digestibility in sheep maintained fauna-free, with total mixed fauna or with Entodinium caudatum monofauna. / Koenig, K M; Ivan, M; Teferedegne, B T; Morgavi, D P; Rode, L M; Ibrahim, I M; Newbold, C J.

In: British Journal of Nutrition, Vol. 98, No. 3, 09.2007, p. 504-16.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Effect of dietary Enterolobium cyclocarpum on microbial protein flow and nutrient digestibility in sheep maintained fauna-free, with total mixed fauna or with Entodinium caudatum monofauna

AU - Koenig, K M

AU - Ivan, M

AU - Teferedegne, B T

AU - Morgavi, D P

AU - Rode, L M

AU - Ibrahim, I M

AU - Newbold, C J

PY - 2007/9

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N2 - Three groups of five wethers with ruminal and duodenal cannulas and maintained as either fauna-free (FF) or inoculated with total mixed fauna (TF) or Entodinium caudatum as a single-species monofauna (EN) were used in an experiment with two 28 d periods. In the first period, the sheep were fed a control barley-based diet (40:60 concentrate to silage DM) and in the second period the diet was supplemented with 187 g DM of Enterolobium cyclocarpum for the last 12 d of the period. The diets of period 1 and 2 were isonitrogenous. There was no effect of fauna on apparent ruminal and total tract organic matter and fibre digestion, but bacterial and microbial N flow and efficiency were improved in FF sheep compared to TF sheep. In period 2, protozoal numbers were reduced between 31 and 88 % 2 h after feeding E. cyclocarpum for the third to twelfth day of supplementation and by an average of 25 % in samples collected over the 24 h feeding cycle. Supplementation of the diet with E. cyclocarpum and the consequent protozoal reduction in TF and EN sheep improved the flow of non-ammonia N and bacterial N to the small intestine and the efficiency of microbial synthesis. However, E. cyclocarpum reduced ruminal organic matter digestion, especially in faunated sheep, and total tract organic matter, N and fibre digestion. Thus, a reduction in the protozoal cell numbers of 25 % was sufficient to achieve the beneficial effects of reduced fauna on the bacterial protein supply, but diet digestibility was reduced.

AB - Three groups of five wethers with ruminal and duodenal cannulas and maintained as either fauna-free (FF) or inoculated with total mixed fauna (TF) or Entodinium caudatum as a single-species monofauna (EN) were used in an experiment with two 28 d periods. In the first period, the sheep were fed a control barley-based diet (40:60 concentrate to silage DM) and in the second period the diet was supplemented with 187 g DM of Enterolobium cyclocarpum for the last 12 d of the period. The diets of period 1 and 2 were isonitrogenous. There was no effect of fauna on apparent ruminal and total tract organic matter and fibre digestion, but bacterial and microbial N flow and efficiency were improved in FF sheep compared to TF sheep. In period 2, protozoal numbers were reduced between 31 and 88 % 2 h after feeding E. cyclocarpum for the third to twelfth day of supplementation and by an average of 25 % in samples collected over the 24 h feeding cycle. Supplementation of the diet with E. cyclocarpum and the consequent protozoal reduction in TF and EN sheep improved the flow of non-ammonia N and bacterial N to the small intestine and the efficiency of microbial synthesis. However, E. cyclocarpum reduced ruminal organic matter digestion, especially in faunated sheep, and total tract organic matter, N and fibre digestion. Thus, a reduction in the protozoal cell numbers of 25 % was sufficient to achieve the beneficial effects of reduced fauna on the bacterial protein supply, but diet digestibility was reduced.

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KW - Ciliophora/isolation & purification

KW - Dietary Fiber/metabolism

KW - Dietary Proteins/metabolism

KW - Dietary Supplements

KW - Digestion/physiology

KW - Duodenum/physiology

KW - Fabaceae/physiology

KW - Fatty Acids, Volatile/analysis

KW - Fermentation/physiology

KW - Gastrointestinal Transit/physiology

KW - Nitrogen/metabolism

KW - Protozoan Proteins/metabolism

KW - Rumen/metabolism

KW - Sheep

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DO - 10.1017/S0007114507723930

M3 - Article

C2 - 17459191

VL - 98

SP - 504

EP - 516

JO - British Journal of Nutrition

JF - British Journal of Nutrition

SN - 0007-1145

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