Effect of pH on the activity of ryegrass Lolium multiflorum proteases

Shirley J.E. Heron; R. Alun Edwards; Patricia Phillips

doi:10.1002/jsfa.2740460304

Effect of pH on the activity of ryegrass Lolium multiflorum proteases

Shirley J.E. Heron^*
, R. Alun Edwards
, Patricia Phillips

^*Corresponding author for this work

Central Faculty

Research output: Contribution to journal › Article › peer-review

65 Citations (Scopus)

Abstract

The effect of pH on plant protease activity was investigated using buffered, gamma‐irradiated aqueous extract of ryegrass Lolium multiflorum. Rapid protein breakdown occurred in the first few days but was followed by a longer period of reduced activity. The overall pH optimum for ryegrass proteases was between 5·0 and 7·0 but there was still considerable activity at the acid pH levels found, eg in silage. Proteolysis did not proceed to completion even when the pH was not inhibiting.

Original language	English
Pages (from-to)	267-277
Number of pages	11
Journal	Journal of the Science of Food and Agriculture
Volume	46
Issue number	3
DOIs	https://doi.org/10.1002/jsfa.2740460304
Publication status	Print publication - 1989

Keywords

pH
proteases
Ryegrass

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10.1002/jsfa.2740460304

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title = "Effect of pH on the activity of ryegrass Lolium multiflorum proteases",

abstract = "The effect of pH on plant protease activity was investigated using buffered, gamma‐irradiated aqueous extract of ryegrass Lolium multiflorum. Rapid protein breakdown occurred in the first few days but was followed by a longer period of reduced activity. The overall pH optimum for ryegrass proteases was between 5·0 and 7·0 but there was still considerable activity at the acid pH levels found, eg in silage. Proteolysis did not proceed to completion even when the pH was not inhibiting.",

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journal = "Journal of the Science of Food and Agriculture",

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AU - Heron, Shirley J.E.

AU - Edwards, R. Alun

AU - Phillips, Patricia

PY - 1989

Y1 - 1989

N2 - The effect of pH on plant protease activity was investigated using buffered, gamma‐irradiated aqueous extract of ryegrass Lolium multiflorum. Rapid protein breakdown occurred in the first few days but was followed by a longer period of reduced activity. The overall pH optimum for ryegrass proteases was between 5·0 and 7·0 but there was still considerable activity at the acid pH levels found, eg in silage. Proteolysis did not proceed to completion even when the pH was not inhibiting.

AB - The effect of pH on plant protease activity was investigated using buffered, gamma‐irradiated aqueous extract of ryegrass Lolium multiflorum. Rapid protein breakdown occurred in the first few days but was followed by a longer period of reduced activity. The overall pH optimum for ryegrass proteases was between 5·0 and 7·0 but there was still considerable activity at the acid pH levels found, eg in silage. Proteolysis did not proceed to completion even when the pH was not inhibiting.

KW - pH

KW - proteases

KW - Ryegrass

UR - https://www.scopus.com/pages/publications/84986820805

U2 - 10.1002/jsfa.2740460304

DO - 10.1002/jsfa.2740460304

M3 - Article

AN - SCOPUS:84986820805

SN - 0022-5142

VL - 46

SP - 267

EP - 277

JO - Journal of the Science of Food and Agriculture

JF - Journal of the Science of Food and Agriculture

IS - 3

ER -

Effect of pH on the activity of ryegrass Lolium multiflorum proteases

Abstract

Keywords

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