Abstract
In the present work, production of hydrogen via dark fermentation has been carried out using the hydrolyzed rice straw and Clostridium pasteurianum (MTCC116). The hydrolysis reaction of 1.0% alkali pretreated rice straw was performed at 70 °C and 10% substrate loading via Fe3O4/Alginate nanocomposite (Fe3O4/Alginate NCs) treated thermostable crude cellulase enzyme following the previously established method. It is noticed that under the optimized conditions, at 70 °C the Fe3O4/Alginate NCs treated cellulase has produced around 54.18 g/L sugars as the rice straw hydrolyzate. Moreover, the efficiency of the process illustrates that using this hydrolyzate, Clostridium pasteurianum (MTCC116) could produce cumulative hydrogen of 2580 ml/L in 144 h with the maximum production rate of 23.96 ml/L/h in 96 h. In addition, maximum dry bacterial biomass of 1.02 g/L and 1.51 g/L was recorded after 96 h and 144 h, respectively with corresponding initial pH of 6.6 and 3.8, suggesting higher hydrogen production.
Original language | English |
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Pages (from-to) | 552-558 |
Number of pages | 7 |
Journal | Bioresource Technology |
Volume | 238 |
Early online date | 21 Apr 2017 |
DOIs | |
Publication status | Print publication - Aug 2017 |
Externally published | Yes |
Keywords
- Aspergillus fumigatus
- Biohydrogen
- Clostridium pasteurianum
- Dark fermentation
- Nanomaterial
- Thermostable cellulases