Electron Beam-Supported Fabrication of Biocompatible Silver/iota-Carrageenan for Wound Healing Application

Siva Sankar Sana, Chaitany Jayprakash Raorane, Vinit Raj, Krishnapandi Alagumalai, Lekshmi Gangadhar, Vijai Kumar Gupta, Seong Cheol Kim*, Ajeet Kumar Kaushik*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Silver nanoparticles (AgNPs) are a potent antibacterial agent, especially when used to treat bacteria that are multidrug resistant. However, it is challenging to eliminate the hazardous reducing agents that remain in AgNPs produced by the conventional chemical reduction process. To overcome these challenges, the presented research demonstrates the fabrication of AgNPs using iota-carrageenan (ι-carra) as a carbohydrate polymer using electron beam (EB) irradiation. Well-characterized ι-carra@AgNPs have a face-centered cubic (FCC) structure with spherical morphology and an average size of 26 nm. Herein we explored the approach for fabricating ι-carra@AgNPs that is suitable for scaling up the production of nanoparticles that exhibit excellent water stability. Further, the optimized ι-carra@AgNPs exhibited considerable antibacterial activity of 40% and 30% inhibition when tested with Gram-negative Escherichia coli ATCC 43895 and Gram-positive Staphylococcus aureus (S. aureus) (ATCC 6538), respectively, and low cytotoxicity at 10-50 μg/mL. To establish the potential biomedical application, as proof of the concept, the ι-carra@AgNPs showed significant antibiofilm activity at 20 μg/mL and also showed 95% wound healing abilities at 50 μg/mL compared to the nontreated control groups. Electron beam assisted ι-carra@AgNPs showed significant beneficial effects against specific bacterial strains and may provide a guide for the development of new antibacterial materials for wound dressing for large-scale production for biomedical applications.

Original languageEnglish
JournalACS Applied Bio Materials
Early online date10 May 2024
DOIs
Publication statusFirst published - 10 May 2024

Bibliographical note

Publisher Copyright:
© 2024 American Chemical Society.

Keywords

  • AgNPs
  • antibacterial activity
  • antibiofilm activity
  • Electron beam
  • wound healing activity
  • ι-carrageenan

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