Electrophoretic variation as a measure of species differentiation between four species of the genus Lolium

Sarita Jane Bennett, Mike D. Hayward, Dave F. Marshall

Research output: Contribution to journalArticleResearchpeer-review

7 Citations (Scopus)

Abstract

Isozyme electrophoresis was carried out on 423 accessions belonging to four species of the genus Lolium to assess the genetic variation within and between the species. Four enzyme systems (acid phosphatase, glutamate oxaloacetate transaminase, phospho-gluco isomerase and superoxide dismutase) were used to array allelic diversity at five polymorphic loci. Nei's genetic distance analysis and multivariate analyses were computed and the taxonomic position of each species is discussed as a result of these computations. Electrophoresis is shown to clearly differentiate the inbreeding species, L. temulentum from the outcrossing species, L. perenne, L. multiflorum and L. rigidum, and to separate the species within the out-breeding group. The taxonomic results are discussed in relation to an earlier paper on morphological variation, concluding that electrophoresis is a valid taxonomic tool showing distinct separations between the species, but that current plant breeding and agricultural practises may be increasing the amount of hybridisation between the species than occurred in the past.
Original languageEnglish
Pages (from-to)59-66
Number of pages8
JournalGenetic Resources and Crop Evolution
Volume49
Issue number1
DOIs
Publication statusPrint publication - 2002
Externally publishedYes

Fingerprint

Lolium
electrophoresis
Electrophoresis
Oxaloacetic Acid
Isomerases
Inbreeding
isomerases
outcrossing
Acid Phosphatase
Transaminases
plant breeding
acid phosphatase
aspartate transaminase
electrokinesis
inbreeding
Isoenzymes
Superoxide Dismutase
Breeding
genetic distance
isozymes

Keywords

  • Isozyme electrophoresis
  • Lolium
  • Multivariate analysis
  • Nei's genetic distance
  • Taxonomy

Cite this

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abstract = "Isozyme electrophoresis was carried out on 423 accessions belonging to four species of the genus Lolium to assess the genetic variation within and between the species. Four enzyme systems (acid phosphatase, glutamate oxaloacetate transaminase, phospho-gluco isomerase and superoxide dismutase) were used to array allelic diversity at five polymorphic loci. Nei's genetic distance analysis and multivariate analyses were computed and the taxonomic position of each species is discussed as a result of these computations. Electrophoresis is shown to clearly differentiate the inbreeding species, L. temulentum from the outcrossing species, L. perenne, L. multiflorum and L. rigidum, and to separate the species within the out-breeding group. The taxonomic results are discussed in relation to an earlier paper on morphological variation, concluding that electrophoresis is a valid taxonomic tool showing distinct separations between the species, but that current plant breeding and agricultural practises may be increasing the amount of hybridisation between the species than occurred in the past.",
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Electrophoretic variation as a measure of species differentiation between four species of the genus Lolium. / Bennett, Sarita Jane; Hayward, Mike D.; Marshall, Dave F.

In: Genetic Resources and Crop Evolution, Vol. 49, No. 1, 2002, p. 59-66.

Research output: Contribution to journalArticleResearchpeer-review

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T1 - Electrophoretic variation as a measure of species differentiation between four species of the genus Lolium

AU - Bennett, Sarita Jane

AU - Hayward, Mike D.

AU - Marshall, Dave F.

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AB - Isozyme electrophoresis was carried out on 423 accessions belonging to four species of the genus Lolium to assess the genetic variation within and between the species. Four enzyme systems (acid phosphatase, glutamate oxaloacetate transaminase, phospho-gluco isomerase and superoxide dismutase) were used to array allelic diversity at five polymorphic loci. Nei's genetic distance analysis and multivariate analyses were computed and the taxonomic position of each species is discussed as a result of these computations. Electrophoresis is shown to clearly differentiate the inbreeding species, L. temulentum from the outcrossing species, L. perenne, L. multiflorum and L. rigidum, and to separate the species within the out-breeding group. The taxonomic results are discussed in relation to an earlier paper on morphological variation, concluding that electrophoresis is a valid taxonomic tool showing distinct separations between the species, but that current plant breeding and agricultural practises may be increasing the amount of hybridisation between the species than occurred in the past.

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