Enhance production of fungal cellulase cocktail using cellulosic waste

Neha Srivastava; Abdallah M. Elgorban; P. K. Mishra; Najat Marraiki; Ahmed M. Alharbi; Irfan Ahmad; Vijai Kumar Gupta

doi:10.1016/j.eti.2020.100949

Enhance production of fungal cellulase cocktail using cellulosic waste

Neha Srivastava^*, Abdallah M. Elgorban, P. K. Mishra, Najat Marraiki, Ahmed M. Alharbi, Irfan Ahmad, Vijai Kumar Gupta^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

23 Citations (Scopus)

Abstract

This study reports improved production of crude fungal cellulase following solid-state fermentation (SSF). The lab isolate fungal strain shows enhanced cellulase production and has been identified as the Cladosporium cladosporioides NS2 using 18S ribosomal DNA sequencing pattern. An initial pH of 4.0 and temperature 30 °C is recorded to be optimum for maximum cellulase production. Among different types of carbon sources used, sugarcane bagasse offers the maximum production of FP (16.9 IU/gds), β-glucosidase (200 IU/gds) and EG (150 IU/gds) activity. In addition, among different types of nitrogen sources, peptone yields maximum production of EG (170 IU/gds), FP (17.5 IU/gds) and β-glucosidase (225 IU/gds). Moreover, among various types of metal ions, manganese ion (Mn⁺⁺) gave the highest production of FP (20 IU/gds), β-glucosidase (240 IU/gds) and EG (210 IU/gds).

Original language	English
Article number	100949
Journal	Environmental Technology and Innovation
Volume	19
Early online date	3 Jun 2020
DOIs	https://doi.org/10.1016/j.eti.2020.100949
Publication status	Print publication - Aug 2020
Externally published	Yes

Keywords

Cellulase production
Cladosporium cladosporioides NS2
Enzymatic hydrolysis
Lignocellulosic biomass
Solid state fermentation

Access to Document

10.1016/j.eti.2020.100949

Cite this

@article{377e3bc133da47bdb6287c8d8d62698a,

title = "Enhance production of fungal cellulase cocktail using cellulosic waste",

abstract = "This study reports improved production of crude fungal cellulase following solid-state fermentation (SSF). The lab isolate fungal strain shows enhanced cellulase production and has been identified as the Cladosporium cladosporioides NS2 using 18S ribosomal DNA sequencing pattern. An initial pH of 4.0 and temperature 30 °C is recorded to be optimum for maximum cellulase production. Among different types of carbon sources used, sugarcane bagasse offers the maximum production of FP (16.9 IU/gds), β-glucosidase (200 IU/gds) and EG (150 IU/gds) activity. In addition, among different types of nitrogen sources, peptone yields maximum production of EG (170 IU/gds), FP (17.5 IU/gds) and β-glucosidase (225 IU/gds). Moreover, among various types of metal ions, manganese ion (Mn++) gave the highest production of FP (20 IU/gds), β-glucosidase (240 IU/gds) and EG (210 IU/gds).",

keywords = "Cellulase production, Cladosporium cladosporioides NS2, Enzymatic hydrolysis, Lignocellulosic biomass, Solid state fermentation",

author = "Neha Srivastava and Elgorban, {Abdallah M.} and Mishra, {P. K.} and Najat Marraiki and Alharbi, {Ahmed M.} and Irfan Ahmad and Gupta, {Vijai Kumar}",

year = "2020",

month = aug,

doi = "10.1016/j.eti.2020.100949",

language = "English",

volume = "19",

journal = "Environmental Technology and Innovation",

issn = "2352-1864",

publisher = "Elsevier",

}

TY - JOUR

T1 - Enhance production of fungal cellulase cocktail using cellulosic waste

AU - Srivastava, Neha

AU - Elgorban, Abdallah M.

AU - Mishra, P. K.

AU - Marraiki, Najat

AU - Alharbi, Ahmed M.

AU - Ahmad, Irfan

AU - Gupta, Vijai Kumar

PY - 2020/8

Y1 - 2020/8

N2 - This study reports improved production of crude fungal cellulase following solid-state fermentation (SSF). The lab isolate fungal strain shows enhanced cellulase production and has been identified as the Cladosporium cladosporioides NS2 using 18S ribosomal DNA sequencing pattern. An initial pH of 4.0 and temperature 30 °C is recorded to be optimum for maximum cellulase production. Among different types of carbon sources used, sugarcane bagasse offers the maximum production of FP (16.9 IU/gds), β-glucosidase (200 IU/gds) and EG (150 IU/gds) activity. In addition, among different types of nitrogen sources, peptone yields maximum production of EG (170 IU/gds), FP (17.5 IU/gds) and β-glucosidase (225 IU/gds). Moreover, among various types of metal ions, manganese ion (Mn++) gave the highest production of FP (20 IU/gds), β-glucosidase (240 IU/gds) and EG (210 IU/gds).

AB - This study reports improved production of crude fungal cellulase following solid-state fermentation (SSF). The lab isolate fungal strain shows enhanced cellulase production and has been identified as the Cladosporium cladosporioides NS2 using 18S ribosomal DNA sequencing pattern. An initial pH of 4.0 and temperature 30 °C is recorded to be optimum for maximum cellulase production. Among different types of carbon sources used, sugarcane bagasse offers the maximum production of FP (16.9 IU/gds), β-glucosidase (200 IU/gds) and EG (150 IU/gds) activity. In addition, among different types of nitrogen sources, peptone yields maximum production of EG (170 IU/gds), FP (17.5 IU/gds) and β-glucosidase (225 IU/gds). Moreover, among various types of metal ions, manganese ion (Mn++) gave the highest production of FP (20 IU/gds), β-glucosidase (240 IU/gds) and EG (210 IU/gds).

KW - Cellulase production

KW - Cladosporium cladosporioides NS2

KW - Enzymatic hydrolysis

KW - Lignocellulosic biomass

KW - Solid state fermentation

UR - http://www.scopus.com/inward/record.url?scp=85086130827&partnerID=8YFLogxK

U2 - 10.1016/j.eti.2020.100949

DO - 10.1016/j.eti.2020.100949

M3 - Article

AN - SCOPUS:85086130827

SN - 2352-1864

VL - 19

JO - Environmental Technology and Innovation

JF - Environmental Technology and Innovation

M1 - 100949

ER -

Enhance production of fungal cellulase cocktail using cellulosic waste

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this