Enhancement in functional stability of microbial endoglanases produced using paddy straw via treatment with manganese oxide based porous nanocomposite synthesized from mixed fruit waste

Neha Srivastava, Rajeev Singh, Manish Srivastava, Akbar Mohammad, Steve Harakeh, Shafiul Haque, P K Mishra, Hossam H Tayeb, Mohammed Moulay, Vijai Kumar Gupta*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Microbial cellulases are the enzymes used in numerous industrial biotechnological applications. Efficiency of celluloytic cocktails plays a key role in the conversion of biomass into biofuels, but limited production, high cost and low efficiency are the main obstacles to sustainable biorefining. The current work aims to establish a feasible approach for boosting the production of fungal endoglucanse (EG) and its functional stability utilizing nanocomposite materials based on manganese oxide. Herein, aqueous extract from mixed fruit waste was used to synthesize the nanocomposite sample, which was subsequently subjected to several characterization techniques for analysis. Following the solid-state fermentation of paddy straw, and by employing 75 mg nanocomposite, 192 IU/gds EG was produced under the optimal conditions, while 19 IU/gds FP and 98 IU/gds BGL production were recorded. The crude EG enzyme treated with nanocomposite also shows complete stability at pH 5.0 for 3.5 h while retaining thermal activity at 70 °C for 4 h.

Original languageEnglish
Article number128219
JournalBioresource Technology
Volume369
Early online date4 Nov 2022
DOIs
Publication statusFirst published - 4 Nov 2022

Bibliographical note

Copyright © 2022 Elsevier Ltd. All rights reserved.

Keywords

  • Enzyme stability
  • Fruit waste
  • Fungal-endoglucanse
  • Manganese oxide nanocomposite
  • Paddy straw

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