Enhancement of fungal cellulase production using pretreated orange peel waste and its application in improved bioconversion of rice husk under the influence of nickel cobaltite nanoparticles

Neha Srivastava; Akbar Mohammad; Dan Bahadur Pal; Manish Srivastava; Mohammad Y. Alshahrani; Irfan Ahmad; Rajeev Singh; P. K. Mishra; Taeho Yoon; Vijai Kumar Gupta

doi:10.1007/s13399-022-03070-3

Enhancement of fungal cellulase production using pretreated orange peel waste and its application in improved bioconversion of rice husk under the influence of nickel cobaltite nanoparticles

Neha Srivastava, Akbar Mohammad, Dan Bahadur Pal, Manish Srivastava^*, Mohammad Y. Alshahrani, Irfan Ahmad, Rajeev Singh, P. K. Mishra, Taeho Yoon, Vijai Kumar Gupta^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

Cellulases are the enzymes of enormous industrial interests. In this study, solid-state fermentation (SSF) of alkali-pretreated orange peel waste has been evaluated to produce fungal cellulase. Different concentrations of alkali solution for the pretreatment of substrate and its impact to maximize the cellulase production have been investigated. Furthermore, process parameters of SSF including different substrate amount, different particle size of substrate, and impact of different moisture contents have been analyzed to achieve maximum cellulase production under the optimized conditions which include pretreatment using 1.0% alkali solution, 7.0 g substrate, 2.2 mm particle size of substrate, and 65% moisture content, wherein fungal strain Emericella variecolor NS3 exhibited the highest cellulase production of 39 IU/gds FP (filter paper cellulase), 236 IU/gds EG (endoglucanase), and 197 IU/gds BGL (β-glucosidase). The produced raw enzyme holds improved thermal and pH stability up to 3.5 h at 65 °C and over pH 4.0–6.0 in the presence of 1.0% nickel cobaltite nanoparticles (NiCo₂O₄ NPs). Consequently, NiCo₂O₄ NP–stabilized enzyme exhibited improved hydrolytic efficiency for alkali-pretreated rice husk as compared to control enzyme system. This work may be potentially applied for the mass-scale production of enzyme using orange wastes at industrial scale. Graphical abstract: [Figure not available: see fulltext.].

Original language	English
Journal	Biomass Conversion and Biorefinery
Early online date	23 Jul 2022
DOIs	https://doi.org/10.1007/s13399-022-03070-3
Publication status	First published - 23 Jul 2022

Bibliographical note

Funding Information:
This study was supported by the Priority Research Centers Program (NRF-2014R1A6A1031189) through the National Research Foundation of Korea (NRF) funded by the Korean Ministry of Education. This work is supported by Scientific Research Deanship at King Khalid University, Abha, Saudi Arabia for their financial support through the Small Research Group Project under grant number (RGP.01-115-43).

Funding Information:
Authors N.S. and P.K.M thankfully acknowledge Department of Chemical Engineering and Technology, IIT (BHU) Varanasi, for providing experimental facilities. The authors are grateful to Scientific Research Deanship at King Khalid University, Abha, Saudi Arabia, for their financial support through the Large Research Group Project under grant number (RGP.02-205-42). Author M.S. acknowledges the Science and Engineering Research Board for SERB Research Scientist award [SB/SRS/2018-19/48/PS] and also to DST for DST INSPIRE Faculty award [IFA-13-MS-02]. V.K.G. would like to acknowledge the institutional research funding supported by the Scotland’s Rural College (SRUC), UK. Credit given to https://pixabay.com for free images to design graphical abstract.

Publisher Copyright:
© 2022, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.

Keywords

Cellulase enzyme
Nanoparticles, enzymatic hydrolysis
Orange peel waste
SSF

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/s13399-022-03070-3

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Srivastava, N., Mohammad, A., Pal, D. B., Srivastava, M., Alshahrani, M. Y., Ahmad, I., Singh, R., Mishra, P. K., Yoon, T., & Gupta, V. K. (2022). Enhancement of fungal cellulase production using pretreated orange peel waste and its application in improved bioconversion of rice husk under the influence of nickel cobaltite nanoparticles. Biomass Conversion and Biorefinery. https://doi.org/10.1007/s13399-022-03070-3

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title = "Enhancement of fungal cellulase production using pretreated orange peel waste and its application in improved bioconversion of rice husk under the influence of nickel cobaltite nanoparticles",

abstract = "Cellulases are the enzymes of enormous industrial interests. In this study, solid-state fermentation (SSF) of alkali-pretreated orange peel waste has been evaluated to produce fungal cellulase. Different concentrations of alkali solution for the pretreatment of substrate and its impact to maximize the cellulase production have been investigated. Furthermore, process parameters of SSF including different substrate amount, different particle size of substrate, and impact of different moisture contents have been analyzed to achieve maximum cellulase production under the optimized conditions which include pretreatment using 1.0% alkali solution, 7.0 g substrate, 2.2 mm particle size of substrate, and 65% moisture content, wherein fungal strain Emericella variecolor NS3 exhibited the highest cellulase production of 39 IU/gds FP (filter paper cellulase), 236 IU/gds EG (endoglucanase), and 197 IU/gds BGL (β-glucosidase). The produced raw enzyme holds improved thermal and pH stability up to 3.5 h at 65 °C and over pH 4.0–6.0 in the presence of 1.0% nickel cobaltite nanoparticles (NiCo2O4 NPs). Consequently, NiCo2O4 NP–stabilized enzyme exhibited improved hydrolytic efficiency for alkali-pretreated rice husk as compared to control enzyme system. This work may be potentially applied for the mass-scale production of enzyme using orange wastes at industrial scale. Graphical abstract: [Figure not available: see fulltext.].",

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author = "Neha Srivastava and Akbar Mohammad and Pal, {Dan Bahadur} and Manish Srivastava and Alshahrani, {Mohammad Y.} and Irfan Ahmad and Rajeev Singh and Mishra, {P. K.} and Taeho Yoon and Gupta, {Vijai Kumar}",

note = "Funding Information: This study was supported by the Priority Research Centers Program (NRF-2014R1A6A1031189) through the National Research Foundation of Korea (NRF) funded by the Korean Ministry of Education. This work is supported by Scientific Research Deanship at King Khalid University, Abha, Saudi Arabia for their financial support through the Small Research Group Project under grant number (RGP.01-115-43). Funding Information: Authors N.S. and P.K.M thankfully acknowledge Department of Chemical Engineering and Technology, IIT (BHU) Varanasi, for providing experimental facilities. The authors are grateful to Scientific Research Deanship at King Khalid University, Abha, Saudi Arabia, for their financial support through the Large Research Group Project under grant number (RGP.02-205-42). Author M.S. acknowledges the Science and Engineering Research Board for SERB Research Scientist award [SB/SRS/2018-19/48/PS] and also to DST for DST INSPIRE Faculty award [IFA-13-MS-02]. V.K.G. would like to acknowledge the institutional research funding supported by the Scotland{\textquoteright}s Rural College (SRUC), UK. Credit given to https://pixabay.com for free images to design graphical abstract. Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.",

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Srivastava, N, Mohammad, A, Pal, DB, Srivastava, M, Alshahrani, MY, Ahmad, I, Singh, R, Mishra, PK, Yoon, T & Gupta, VK 2022, 'Enhancement of fungal cellulase production using pretreated orange peel waste and its application in improved bioconversion of rice husk under the influence of nickel cobaltite nanoparticles', Biomass Conversion and Biorefinery. https://doi.org/10.1007/s13399-022-03070-3

Enhancement of fungal cellulase production using pretreated orange peel waste and its application in improved bioconversion of rice husk under the influence of nickel cobaltite nanoparticles. / Srivastava, Neha; Mohammad, Akbar; Pal, Dan Bahadur et al.

In: Biomass Conversion and Biorefinery, 23.07.2022.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Enhancement of fungal cellulase production using pretreated orange peel waste and its application in improved bioconversion of rice husk under the influence of nickel cobaltite nanoparticles

AU - Srivastava, Neha

AU - Mohammad, Akbar

AU - Pal, Dan Bahadur

AU - Srivastava, Manish

AU - Alshahrani, Mohammad Y.

AU - Ahmad, Irfan

AU - Singh, Rajeev

AU - Mishra, P. K.

AU - Yoon, Taeho

AU - Gupta, Vijai Kumar

N1 - Funding Information: This study was supported by the Priority Research Centers Program (NRF-2014R1A6A1031189) through the National Research Foundation of Korea (NRF) funded by the Korean Ministry of Education. This work is supported by Scientific Research Deanship at King Khalid University, Abha, Saudi Arabia for their financial support through the Small Research Group Project under grant number (RGP.01-115-43). Funding Information: Authors N.S. and P.K.M thankfully acknowledge Department of Chemical Engineering and Technology, IIT (BHU) Varanasi, for providing experimental facilities. The authors are grateful to Scientific Research Deanship at King Khalid University, Abha, Saudi Arabia, for their financial support through the Large Research Group Project under grant number (RGP.02-205-42). Author M.S. acknowledges the Science and Engineering Research Board for SERB Research Scientist award [SB/SRS/2018-19/48/PS] and also to DST for DST INSPIRE Faculty award [IFA-13-MS-02]. V.K.G. would like to acknowledge the institutional research funding supported by the Scotland’s Rural College (SRUC), UK. Credit given to https://pixabay.com for free images to design graphical abstract. Publisher Copyright: © 2022, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.

PY - 2022/7/23

Y1 - 2022/7/23

N2 - Cellulases are the enzymes of enormous industrial interests. In this study, solid-state fermentation (SSF) of alkali-pretreated orange peel waste has been evaluated to produce fungal cellulase. Different concentrations of alkali solution for the pretreatment of substrate and its impact to maximize the cellulase production have been investigated. Furthermore, process parameters of SSF including different substrate amount, different particle size of substrate, and impact of different moisture contents have been analyzed to achieve maximum cellulase production under the optimized conditions which include pretreatment using 1.0% alkali solution, 7.0 g substrate, 2.2 mm particle size of substrate, and 65% moisture content, wherein fungal strain Emericella variecolor NS3 exhibited the highest cellulase production of 39 IU/gds FP (filter paper cellulase), 236 IU/gds EG (endoglucanase), and 197 IU/gds BGL (β-glucosidase). The produced raw enzyme holds improved thermal and pH stability up to 3.5 h at 65 °C and over pH 4.0–6.0 in the presence of 1.0% nickel cobaltite nanoparticles (NiCo2O4 NPs). Consequently, NiCo2O4 NP–stabilized enzyme exhibited improved hydrolytic efficiency for alkali-pretreated rice husk as compared to control enzyme system. This work may be potentially applied for the mass-scale production of enzyme using orange wastes at industrial scale. Graphical abstract: [Figure not available: see fulltext.].

AB - Cellulases are the enzymes of enormous industrial interests. In this study, solid-state fermentation (SSF) of alkali-pretreated orange peel waste has been evaluated to produce fungal cellulase. Different concentrations of alkali solution for the pretreatment of substrate and its impact to maximize the cellulase production have been investigated. Furthermore, process parameters of SSF including different substrate amount, different particle size of substrate, and impact of different moisture contents have been analyzed to achieve maximum cellulase production under the optimized conditions which include pretreatment using 1.0% alkali solution, 7.0 g substrate, 2.2 mm particle size of substrate, and 65% moisture content, wherein fungal strain Emericella variecolor NS3 exhibited the highest cellulase production of 39 IU/gds FP (filter paper cellulase), 236 IU/gds EG (endoglucanase), and 197 IU/gds BGL (β-glucosidase). The produced raw enzyme holds improved thermal and pH stability up to 3.5 h at 65 °C and over pH 4.0–6.0 in the presence of 1.0% nickel cobaltite nanoparticles (NiCo2O4 NPs). Consequently, NiCo2O4 NP–stabilized enzyme exhibited improved hydrolytic efficiency for alkali-pretreated rice husk as compared to control enzyme system. This work may be potentially applied for the mass-scale production of enzyme using orange wastes at industrial scale. Graphical abstract: [Figure not available: see fulltext.].

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KW - Nanoparticles, enzymatic hydrolysis

KW - Orange peel waste

KW - SSF

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DO - 10.1007/s13399-022-03070-3

M3 - Article

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SN - 2190-6815

JO - Biomass Conversion and Biorefinery

JF - Biomass Conversion and Biorefinery

ER -

Enhancement of fungal cellulase production using pretreated orange peel waste and its application in improved bioconversion of rice husk under the influence of nickel cobaltite nanoparticles

Abstract

Bibliographical note

Keywords

UN SDGs

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