TY - JOUR
T1 - Enhancement of fungal cellulase production using pretreated orange peel waste and its application in improved bioconversion of rice husk under the influence of nickel cobaltite nanoparticles
AU - Srivastava, Neha
AU - Mohammad, Akbar
AU - Pal, Dan Bahadur
AU - Srivastava, Manish
AU - Alshahrani, Mohammad Y.
AU - Ahmad, Irfan
AU - Singh, Rajeev
AU - Mishra, P. K.
AU - Yoon, Taeho
AU - Gupta, Vijai Kumar
N1 - Publisher Copyright:
© The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2022.
PY - 2024/3
Y1 - 2024/3
N2 - Cellulases are the enzymes of enormous industrial interests. In this study, solid-state fermentation (SSF) of alkali-pretreated orange peel waste has been evaluated to produce fungal cellulase. Different concentrations of alkali solution for the pretreatment of substrate and its impact to maximize the cellulase production have been investigated. Furthermore, process parameters of SSF including different substrate amount, different particle size of substrate, and impact of different moisture contents have been analyzed to achieve maximum cellulase production under the optimized conditions which include pretreatment using 1.0% alkali solution, 7.0 g substrate, 2.2 mm particle size of substrate, and 65% moisture content, wherein fungal strain Emericella variecolor NS3 exhibited the highest cellulase production of 39 IU/gds FP (filter paper cellulase), 236 IU/gds EG (endoglucanase), and 197 IU/gds BGL (β-glucosidase). The produced raw enzyme holds improved thermal and pH stability up to 3.5 h at 65 °C and over pH 4.0–6.0 in the presence of 1.0% nickel cobaltite nanoparticles (NiCo
2O
4 NPs). Consequently, NiCo
2O
4 NP–stabilized enzyme exhibited improved hydrolytic efficiency for alkali-pretreated rice husk as compared to control enzyme system. This work may be potentially applied for the mass-scale production of enzyme using orange wastes at industrial scale. Graphical abstract: (Figure presented.)
AB - Cellulases are the enzymes of enormous industrial interests. In this study, solid-state fermentation (SSF) of alkali-pretreated orange peel waste has been evaluated to produce fungal cellulase. Different concentrations of alkali solution for the pretreatment of substrate and its impact to maximize the cellulase production have been investigated. Furthermore, process parameters of SSF including different substrate amount, different particle size of substrate, and impact of different moisture contents have been analyzed to achieve maximum cellulase production under the optimized conditions which include pretreatment using 1.0% alkali solution, 7.0 g substrate, 2.2 mm particle size of substrate, and 65% moisture content, wherein fungal strain Emericella variecolor NS3 exhibited the highest cellulase production of 39 IU/gds FP (filter paper cellulase), 236 IU/gds EG (endoglucanase), and 197 IU/gds BGL (β-glucosidase). The produced raw enzyme holds improved thermal and pH stability up to 3.5 h at 65 °C and over pH 4.0–6.0 in the presence of 1.0% nickel cobaltite nanoparticles (NiCo
2O
4 NPs). Consequently, NiCo
2O
4 NP–stabilized enzyme exhibited improved hydrolytic efficiency for alkali-pretreated rice husk as compared to control enzyme system. This work may be potentially applied for the mass-scale production of enzyme using orange wastes at industrial scale. Graphical abstract: (Figure presented.)
KW - Cellulase enzyme
KW - Nanoparticles, enzymatic hydrolysis
KW - Orange peel waste
KW - SSF
UR - http://www.scopus.com/inward/record.url?scp=85135014241&partnerID=8YFLogxK
U2 - 10.1007/s13399-022-03070-3
DO - 10.1007/s13399-022-03070-3
M3 - Article
AN - SCOPUS:85135014241
SN - 2190-6815
VL - 14
SP - 6687
EP - 6696
JO - Biomass Conversion and Biorefinery
JF - Biomass Conversion and Biorefinery
IS - 5
ER -
