Enhancement of fungal cellulase production using pretreated orange peel waste and its application in improved bioconversion of rice husk under the influence of nickel cobaltite nanoparticles

Neha Srivastava, Akbar Mohammad, Dan Bahadur Pal, Manish Srivastava*, Mohammad Y. Alshahrani, Irfan Ahmad, Rajeev Singh, P. K. Mishra, Taeho Yoon, Vijai Kumar Gupta*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Citations (Scopus)

Abstract

Cellulases are the enzymes of enormous industrial interests. In this study, solid-state fermentation (SSF) of alkali-pretreated orange peel waste has been evaluated to produce fungal cellulase. Different concentrations of alkali solution for the pretreatment of substrate and its impact to maximize the cellulase production have been investigated. Furthermore, process parameters of SSF including different substrate amount, different particle size of substrate, and impact of different moisture contents have been analyzed to achieve maximum cellulase production under the optimized conditions which include pretreatment using 1.0% alkali solution, 7.0 g substrate, 2.2 mm particle size of substrate, and 65% moisture content, wherein fungal strain Emericella variecolor NS3 exhibited the highest cellulase production of 39 IU/gds FP (filter paper cellulase), 236 IU/gds EG (endoglucanase), and 197 IU/gds BGL (β-glucosidase). The produced raw enzyme holds improved thermal and pH stability up to 3.5 h at 65 °C and over pH 4.0–6.0 in the presence of 1.0% nickel cobaltite nanoparticles (NiCo2O4 NPs). Consequently, NiCo2O4 NP–stabilized enzyme exhibited improved hydrolytic efficiency for alkali-pretreated rice husk as compared to control enzyme system. This work may be potentially applied for the mass-scale production of enzyme using orange wastes at industrial scale. Graphical abstract: [Figure not available: see fulltext.].

Original languageEnglish
JournalBiomass Conversion and Biorefinery
Early online date23 Jul 2022
DOIs
Publication statusFirst published - 23 Jul 2022

Bibliographical note

Funding Information:
This study was supported by the Priority Research Centers Program (NRF-2014R1A6A1031189) through the National Research Foundation of Korea (NRF) funded by the Korean Ministry of Education. This work is supported by Scientific Research Deanship at King Khalid University, Abha, Saudi Arabia for their financial support through the Small Research Group Project under grant number (RGP.01-115-43).

Funding Information:
Authors N.S. and P.K.M thankfully acknowledge Department of Chemical Engineering and Technology, IIT (BHU) Varanasi, for providing experimental facilities. The authors are grateful to Scientific Research Deanship at King Khalid University, Abha, Saudi Arabia, for their financial support through the Large Research Group Project under grant number (RGP.02-205-42). Author M.S. acknowledges the Science and Engineering Research Board for SERB Research Scientist award [SB/SRS/2018-19/48/PS] and also to DST for DST INSPIRE Faculty award [IFA-13-MS-02]. V.K.G. would like to acknowledge the institutional research funding supported by the Scotland’s Rural College (SRUC), UK. Credit given to https://pixabay.com for free images to design graphical abstract.

Publisher Copyright:
© 2022, The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.

Keywords

  • Cellulase enzyme
  • Nanoparticles, enzymatic hydrolysis
  • Orange peel waste
  • SSF

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