Enzymatic synthesis of N-acetyllactosamine from lactose enabled by recombinant β1,4-galactosyltransferases

Kun Huang, Fabio Parmeggiani, Helene Ledru, Kristian Hollingsworth, Jordi Mas Pons, Andrea Marchesi, Peter Both, Ashley P Mattey, Edward Pallister, Gregory S Bulmer, Jolanda M van Munster, W Bruce Turnbull, M Carmen Galan, Sabine L Flitsch

Research output: Contribution to journalArticle

4 Citations (Scopus)


Utilising a fast and sensitive screening method based on imidazolium-tagged probes, we report unprecedented reversible activity of bacterial β1,4-galactosyltransferases to catalyse the transgalactosylation from lactose to N-acetylglucosamine to form N-acetyllactosamine in the presence of UDP. The process is demonstrated by the preparative scale synthesis of pNP-β-LacNAc from lactose using β1,4-galactosyltransferase NmLgtB-B as the only biocatalyst.

Original languageEnglish
Pages (from-to)5920-5924
Number of pages5
JournalOrganic and Biomolecular Chemistry
Issue number24
Early online date30 May 2019
Publication statusFirst published - 30 May 2019
Externally publishedYes


  • Amino Sugars/biosynthesis
  • Biocatalysis
  • Galactosyltransferases/chemistry
  • Lactose/chemistry
  • Molecular Structure
  • Recombinant Proteins/chemistry

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    Huang, K., Parmeggiani, F., Ledru, H., Hollingsworth, K., Mas Pons, J., Marchesi, A., Both, P., Mattey, A. P., Pallister, E., Bulmer, G. S., van Munster, J. M., Turnbull, W. B., Galan, M. C., & Flitsch, S. L. (2019). Enzymatic synthesis of N-acetyllactosamine from lactose enabled by recombinant β1,4-galactosyltransferases. Organic and Biomolecular Chemistry, 17(24), 5920-5924. https://doi.org/10.1039/c9ob01089k