Evaluation of enhanced production of cellulose deconstructing enzyme using natural and alkali pretreated sugar cane bagasse under the influence of graphene oxide

Neha Srivastava; Akbar Mohammad; Rajeev Singh; Manish Srivastava; Asad Syed; Dan Bahadur Pal; Abdallah M. Elgorban; P. K. Mishra; Vijai Kumar Gupta

doi:10.1016/j.biortech.2021.126015

Evaluation of enhanced production of cellulose deconstructing enzyme using natural and alkali pretreated sugar cane bagasse under the influence of graphene oxide

Neha Srivastava, Akbar Mohammad, Rajeev Singh, Manish Srivastava, Asad Syed, Dan Bahadur Pal, Abdallah M. Elgorban, P. K. Mishra, Vijai Kumar Gupta^*

^*Corresponding author for this work

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Abstract

High production cost of cellulase enzyme is one of the main constraints in the practical implementation of biofuels at global scale. Therefore, the present investigation is focused to produce low-cost cellulase via sustainable strategies. This work evaluates to achieve enhanced fungal cellulase production using natural and pretreated sugar cane bagasse (SCB) via Rhizopus oryzae NS5 under the solid state fermentation (SSF) while implementing graphene oxide (GO) as a catalyst. A low alkali treatment showed better performance for cellulase production wherein 14 IU/gds FP activity is observed in 96 h using 0.5% alkali treated SCB, significantly higher as compared to 10 IU/gds FP in case of untreated SCB. Further, the effect of GO has been investigated on cellulase production, incubation temperature and pH of the production medium. Under the influence of 1.5% concentration of GO, alkali pretreated SCB produced maximum 25 IU/gds cellulase in 72 h at pH 5.0 and 40 °C.

Original language	English
Article number	126015
Journal	Bioresource Technology
Volume	342
Early online date	22 Sept 2021
DOIs	https://doi.org/10.1016/j.biortech.2021.126015
Publication status	Print publication - Dec 2021

Bibliographical note

Keywords

Bioconversion, graphene oxide
Cellulases
Rhizopus oryzae
Solid state fermentation
Sugarcane bagasse

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.biortech.2021.126015

evaluation of enhance productionAccepted author manuscript, 1.84 MBLicence: CC BY-NC-ND

Cite this

Srivastava, N., Mohammad, A., Singh, R., Srivastava, M., Syed, A., Bahadur Pal, D., Elgorban, A. M., Mishra, P. K., & Gupta, V. K. (2021). Evaluation of enhanced production of cellulose deconstructing enzyme using natural and alkali pretreated sugar cane bagasse under the influence of graphene oxide. Bioresource Technology, 342, Article 126015. https://doi.org/10.1016/j.biortech.2021.126015

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title = "Evaluation of enhanced production of cellulose deconstructing enzyme using natural and alkali pretreated sugar cane bagasse under the influence of graphene oxide",

abstract = "High production cost of cellulase enzyme is one of the main constraints in the practical implementation of biofuels at global scale. Therefore, the present investigation is focused to produce low-cost cellulase via sustainable strategies. This work evaluates to achieve enhanced fungal cellulase production using natural and pretreated sugar cane bagasse (SCB) via Rhizopus oryzae NS5 under the solid state fermentation (SSF) while implementing graphene oxide (GO) as a catalyst. A low alkali treatment showed better performance for cellulase production wherein 14 IU/gds FP activity is observed in 96 h using 0.5% alkali treated SCB, significantly higher as compared to 10 IU/gds FP in case of untreated SCB. Further, the effect of GO has been investigated on cellulase production, incubation temperature and pH of the production medium. Under the influence of 1.5% concentration of GO, alkali pretreated SCB produced maximum 25 IU/gds cellulase in 72 h at pH 5.0 and 40 °C.",

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Srivastava, N, Mohammad, A, Singh, R, Srivastava, M, Syed, A, Bahadur Pal, D, Elgorban, AM, Mishra, PK & Gupta, VK 2021, 'Evaluation of enhanced production of cellulose deconstructing enzyme using natural and alkali pretreated sugar cane bagasse under the influence of graphene oxide', Bioresource Technology, vol. 342, 126015. https://doi.org/10.1016/j.biortech.2021.126015

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AU - Srivastava, Neha

AU - Mohammad, Akbar

AU - Singh, Rajeev

AU - Srivastava, Manish

AU - Syed, Asad

AU - Bahadur Pal, Dan

AU - Elgorban, Abdallah M.

AU - Mishra, P. K.

AU - Gupta, Vijai Kumar

PY - 2021/12

Y1 - 2021/12

N2 - High production cost of cellulase enzyme is one of the main constraints in the practical implementation of biofuels at global scale. Therefore, the present investigation is focused to produce low-cost cellulase via sustainable strategies. This work evaluates to achieve enhanced fungal cellulase production using natural and pretreated sugar cane bagasse (SCB) via Rhizopus oryzae NS5 under the solid state fermentation (SSF) while implementing graphene oxide (GO) as a catalyst. A low alkali treatment showed better performance for cellulase production wherein 14 IU/gds FP activity is observed in 96 h using 0.5% alkali treated SCB, significantly higher as compared to 10 IU/gds FP in case of untreated SCB. Further, the effect of GO has been investigated on cellulase production, incubation temperature and pH of the production medium. Under the influence of 1.5% concentration of GO, alkali pretreated SCB produced maximum 25 IU/gds cellulase in 72 h at pH 5.0 and 40 °C.

AB - High production cost of cellulase enzyme is one of the main constraints in the practical implementation of biofuels at global scale. Therefore, the present investigation is focused to produce low-cost cellulase via sustainable strategies. This work evaluates to achieve enhanced fungal cellulase production using natural and pretreated sugar cane bagasse (SCB) via Rhizopus oryzae NS5 under the solid state fermentation (SSF) while implementing graphene oxide (GO) as a catalyst. A low alkali treatment showed better performance for cellulase production wherein 14 IU/gds FP activity is observed in 96 h using 0.5% alkali treated SCB, significantly higher as compared to 10 IU/gds FP in case of untreated SCB. Further, the effect of GO has been investigated on cellulase production, incubation temperature and pH of the production medium. Under the influence of 1.5% concentration of GO, alkali pretreated SCB produced maximum 25 IU/gds cellulase in 72 h at pH 5.0 and 40 °C.

KW - Bioconversion, graphene oxide

KW - Cellulases

KW - Rhizopus oryzae

KW - Solid state fermentation

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SN - 0960-8524

VL - 342

JO - Bioresource Technology

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Evaluation of enhanced production of cellulose deconstructing enzyme using natural and alkali pretreated sugar cane bagasse under the influence of graphene oxide

Abstract

Bibliographical note

Keywords

UN SDGs

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