Evidence for seed transmission and symptomless growth of Ramularia collo-cygni in barley (Hordeum vulgare)

ND Havis, M Nyman, SJP Oxley

Research output: Contribution to journalArticle

19 Citations (Scopus)
1 Downloads (Pure)

Abstract

Ramularia collo-cygni (Rcc) is becoming an increasing problem for barley growers across Europe. However, the life cycle of the pathogen is only slowly being elucidated. In this study, Rcc DNA was detected in a number of harvested seed samples from 1999 to 2010, with mean levels peaking in winter barley samples in 2009. A number of experiments were carried out to determine whether the pathogen could move from barley seed to seedlings, and also from seed through the developing plant and into the subsequent generation of seed, both in controlled experiments and in field trials. Results from testing of seed indicated that the fungus is widespread at the end of the growing season in harvested grain samples and can be transmitted to developing plants from infected seed stock. Examination of infected seedlings did not reveal the presence of spores but fungal structures were found within the leaf. The location of the fungus within seed was examined, with Rcc DNA found in both embryo and non-embryo tissue. The implications for barley production of the pathogen being seedborne are discussed.
Original languageEnglish
Pages (from-to)929 - 936
Number of pages8
JournalPlant Pathology
Volume63
Issue number4
DOIs
Publication statusPrint publication - Aug 2014

Fingerprint

Ramularia
Cygnus
Hordeum vulgare
barley
seeds
pathogens
winter barley
fungal spores
DNA
sampling
embryo (plant)
growers
growing season
fungi
seedlings
leaves

Bibliographical note

1023370

Keywords

  • Controlled environment
  • Field experiment
  • PCR
  • Pathogen movement
  • Ramularia leaf spot
  • Seed infection

Cite this

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abstract = "Ramularia collo-cygni (Rcc) is becoming an increasing problem for barley growers across Europe. However, the life cycle of the pathogen is only slowly being elucidated. In this study, Rcc DNA was detected in a number of harvested seed samples from 1999 to 2010, with mean levels peaking in winter barley samples in 2009. A number of experiments were carried out to determine whether the pathogen could move from barley seed to seedlings, and also from seed through the developing plant and into the subsequent generation of seed, both in controlled experiments and in field trials. Results from testing of seed indicated that the fungus is widespread at the end of the growing season in harvested grain samples and can be transmitted to developing plants from infected seed stock. Examination of infected seedlings did not reveal the presence of spores but fungal structures were found within the leaf. The location of the fungus within seed was examined, with Rcc DNA found in both embryo and non-embryo tissue. The implications for barley production of the pathogen being seedborne are discussed.",
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Evidence for seed transmission and symptomless growth of Ramularia collo-cygni in barley (Hordeum vulgare). / Havis, ND; Nyman, M; Oxley, SJP.

In: Plant Pathology, Vol. 63, No. 4, 08.2014, p. 929 - 936.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Evidence for seed transmission and symptomless growth of Ramularia collo-cygni in barley (Hordeum vulgare)

AU - Havis, ND

AU - Nyman, M

AU - Oxley, SJP

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PY - 2014/8

Y1 - 2014/8

N2 - Ramularia collo-cygni (Rcc) is becoming an increasing problem for barley growers across Europe. However, the life cycle of the pathogen is only slowly being elucidated. In this study, Rcc DNA was detected in a number of harvested seed samples from 1999 to 2010, with mean levels peaking in winter barley samples in 2009. A number of experiments were carried out to determine whether the pathogen could move from barley seed to seedlings, and also from seed through the developing plant and into the subsequent generation of seed, both in controlled experiments and in field trials. Results from testing of seed indicated that the fungus is widespread at the end of the growing season in harvested grain samples and can be transmitted to developing plants from infected seed stock. Examination of infected seedlings did not reveal the presence of spores but fungal structures were found within the leaf. The location of the fungus within seed was examined, with Rcc DNA found in both embryo and non-embryo tissue. The implications for barley production of the pathogen being seedborne are discussed.

AB - Ramularia collo-cygni (Rcc) is becoming an increasing problem for barley growers across Europe. However, the life cycle of the pathogen is only slowly being elucidated. In this study, Rcc DNA was detected in a number of harvested seed samples from 1999 to 2010, with mean levels peaking in winter barley samples in 2009. A number of experiments were carried out to determine whether the pathogen could move from barley seed to seedlings, and also from seed through the developing plant and into the subsequent generation of seed, both in controlled experiments and in field trials. Results from testing of seed indicated that the fungus is widespread at the end of the growing season in harvested grain samples and can be transmitted to developing plants from infected seed stock. Examination of infected seedlings did not reveal the presence of spores but fungal structures were found within the leaf. The location of the fungus within seed was examined, with Rcc DNA found in both embryo and non-embryo tissue. The implications for barley production of the pathogen being seedborne are discussed.

KW - Controlled environment

KW - Field experiment

KW - PCR

KW - Pathogen movement

KW - Ramularia leaf spot

KW - Seed infection

U2 - 10.1111/ppa.12162

DO - 10.1111/ppa.12162

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