Evolutionary dynamics of the cellulose synthase gene superfamily in grasses

Julian G. Schwerdt, Katrin MacKenzie, Frank Wright, Daniel Oehme, John M. Wagner, Andrew J. Harvey, Neil J. Shirley, Rachel A. Burton, Miriam Schreiber, Claire Halpin, Jochen Zimmer, David F. Marshall, Robbie Waugh, Geoffrey B. Fincher

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Abstract

Phylogenetic analyses of cellulose synthase (CesA) and cellulose synthase-like (Csl) families from the cellulose synthase gene
superfamily were used to reconstruct their evolutionary origins and selection histories. Counterintuitively, genes encoding
primary cell wall CesAs have undergone extensive expansion and diversification following an ancestral duplication from a
secondary cell wall-associated CesA. Selection pressure across entire CesA and Csl clades appears to be low, but this conceals
considerable variation within individual clades. Genes in the CslF clade are of particular interest because some mediate the
synthesis of (1,3;1,4)-b-glucan, a polysaccharide characteristic of the evolutionarily successful grasses that is not widely
distributed elsewhere in the plant kingdom. The phylogeny suggests that duplication of either CslF6 and/or CslF7 produced the
ancestor of a highly conserved cluster of CslF genes that remain located in syntenic regions of all the grass genomes examined.
A CslF6-specific insert encoding approximately 55 amino acid residues has subsequently been incorporated into the gene, or possibly
lost from other CslFs, and the CslF7 clade has undergone a significant long-term shift in selection pressure. Homology modeling and
molecular dynamics of the CslF6 protein were used to define the three-dimensional dispositions of individual amino acids that are
subject to strong ongoing selection, together with the position of the conserved 55-amino acid insert that is known to influence the
amounts and fine structures of (1,3;1,4)-b-glucans synthesized. These wall polysaccharides are attracting renewed interest because of
their central roles as sources of dietary fiber in human health and for the generation of renewable liquid biofuels.
Original languageEnglish
Pages (from-to)968-983
JournalPlant Physiology
Volume168
Early online date21 May 2015
DOIs
Publication statusPrint publication - Jul 2015
Externally publishedYes

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    Schwerdt, J. G., MacKenzie, K., Wright, F., Oehme, D., Wagner, J. M., Harvey, A. J., Shirley, N. J., Burton, R. A., Schreiber, M., Halpin, C., Zimmer, J., Marshall, D. F., Waugh, R., & Fincher, G. B. (2015). Evolutionary dynamics of the cellulose synthase gene superfamily in grasses. Plant Physiology, 168, 968-983. https://doi.org/10.1104/pp.15.00140