Exploring nano-enabled CRISPR-Cas-powered strategies for efficient diagnostics and treatment of infectious diseases

Ankit Kumar Dubey, Vijai Kumar Gupta, Małgorzata Kujawska, Gorka Orive, Nam-Young Kim, Chen-Zhong Li, Yogendra Kumar Mishra, Ajeet Kaushik*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

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Abstract

Biomedical researchers have subsequently been inspired the development of new approaches for precisely changing an organism's genomic DNA in order to investigate customized diagnostics and therapeutics utilizing genetic engineering techniques. Clustered Regulatory Interspaced Short Palindromic Repeats (CRISPR) is one such technique that has emerged as a safe, targeted, and effective pharmaceutical treatment against a wide range of disease-causing organisms, including bacteria, fungi, parasites, and viruses, as well as genetic abnormalities. The recent discovery of very flexible engineered nucleic acid binding proteins has changed the scientific area of genome editing in a revolutionary way. Since current genetic engineering technique relies on viral vectors, issues about immunogenicity, insertional oncogenesis, retention, and targeted delivery remain unanswered. The use of nanotechnology has the potential to improve the safety and efficacy of CRISPR/Cas9 component distribution by employing tailored polymeric nanoparticles. The combination of two (CRISPR/Cas9 and nanotechnology) offers the potential to open new therapeutic paths. Considering the benefits, demand, and constraints, the goal of this research is to acquire more about the biology of CRISPR technology, as well as aspects of selective and effective diagnostics and therapies for infectious illnesses and other metabolic disorders. This review advocated combining nanomedicine (nanomedicine) with a CRISPR/Cas enabled sensing system to perform early-stage diagnostics and selective therapy of specific infectious disorders. Such a Nano-CRISPR-powered nanomedicine and sensing system would allow for successful infectious illness control, even on a personal level. This comprehensive study also discusses the current obstacles and potential of the predicted technology.

Graphical abstract:

Supplementary Information: The online version contains supplementary material available at 10.1007/s40097-022-00472-7.

Original languageEnglish
Pages (from-to)1-32
Number of pages32
JournalJournal of Nanostructure in Chemistry
Early online date14 Feb 2022
DOIs
Publication statusFirst published - 14 Feb 2022

Bibliographical note

© The Author(s), under exclusive licence to Islamic Azad University 2022.

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