Expression characteristics of pineal miRNAs at ovine different reproductive stages and the identification of miRNAs targeting the AANAT gene

Ran Di, Qiu Yue Liu, Shu Hui Song, Dong Mei Tian, Jian Ning He, Ying Ge, Xiang Yu Wang, Wen Ping Hu, Joram Mwashigadi Mwacharo, Zhang Yuan Pan, Jian Dong Wang, Qing Ma, Gui Ling Cao, Hui Hui Jin, Xiao Jun Liang*, Ming Xing Chu*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

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Abstract

Background: Many recent studies have shown that miRNAs play important roles in the regulation of animal reproduction, including seasonal reproduction. The pineal gland is a crucial hub in the regulation of seasonal reproduction. However, little is known about the expression characteristics of pineal miRNAs in different reproductive seasons (anestrus and breeding season). Therefore, the expression profiles and regulatory roles of ovine pineal miRNAs were investigated during different reproductive stages using Solexa sequencing technology and dual luciferase reporter assays. Results: A total of 427 miRNAs were identified in the sheep pineal gland. Significant differences in miRNA expression were demonstrated between anestrus and the breeding season in terms of the frequency distributions of miRNA lengths, number of expressed miRNAs, and specifically and highly expressed miRNAs in each reproductive stage. KEGG analysis of the differentially expressed (DE) miRNAs between anestrus and the breeding season indicated that they are significantly enriched in pathways related to protein synthesis, secretion and uptake. Furthermore, transcriptome analysis revealed that many target genes of DE miRNAs in the ribosome pathway showed relatively low expression in the breeding season. On the other hand, analyses combining miRNA-gene expression data with target relationship validation in vitro implied that miR-89 may participate in the negative regulation of aralkylamine N-acetyltransferase (AANAT) mRNA expression by targeting its 3’UTR at a unique binding site. Conclusions: Our results provide new insights into the expression characteristics of sheep pineal miRNAs at different reproductive stages and into the negative regulatory effects of pineal miRNAs on AANAT mRNA expression.

Original languageEnglish
Article number217
JournalBMC Genomics
Volume22
Early online date25 Mar 2021
DOIs
Publication statusPrint publication - May 2021
Externally publishedYes

Keywords

  • Anestrus
  • Breeding season
  • miRNAs
  • Pineal gland
  • Sheep

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