Field and pulping performances of transgenic trees with altered lignification

Gilles Pilate, Emma Guiney, Karen Holt, Michel Petit-Conil, Catherine Lapierre, Jean-Charles Leplé, Brigitte Pollet, Isabelle Mila, Elizabeth A Webster, Håkan G Marstorp, David W Hopkins, Lise Jouanin, Wout Boerjan, Wolfgang Schuch, Daniel Cornu, Claire Halpin

Research output: Contribution to journalArticleResearchpeer-review

283 Citations (Scopus)

Abstract

The agronomic and pulping performance of transgenic trees with altered lignin has been evaluated in duplicated, long-term field trials. Poplars expressing cinnamyl alcohol dehydrogenase (CAD) or caffeate/5-hydroxy-ferulate O-methyltransferase (COMT) antisense transgenes were grown for four years at two sites, in France and England. The trees remained healthy throughout the trial. Growth indicators and interactions with insects were normal. No changes in soil microbial communities were detected beneath the transgenic trees. The expected modifications to lignin were maintained in the transgenics over four years, at both sites. Kraft pulping of tree trunks showed that the reduced-CAD lines had improved characteristics, allowing easier delignification, using smaller amounts of chemicals, while yielding more high-quality pulp. This work highlights the potential of engineering wood quality for more environmentally benign papermaking without interfering with tree growth or fitness.

Original languageEnglish
Pages (from-to)607-612
Number of pages6
JournalNature Biotechnology
Volume20
Issue number6
DOIs
Publication statusPrint publication - Jun 2002
Externally publishedYes

Fingerprint

Lignin
Alcohols
Kraft process
Delignification
Papermaking
Methyltransferases
Pulp
Wood
Soils
Growth
Transgenes
England
France
Insects
Soil
Oxidoreductases
cinnamyl alcohol dehydrogenase

Keywords

  • Alcohol oxidoreductases
  • Antisense elements
  • Ecosystem
  • Environmental pollution
  • France
  • Gene expression
  • Industrial waste
  • Lignin
  • Methyltransferases
  • Paper
  • Soil microbiology
  • Transgenes
  • United Kingdom
  • Wood

Cite this

Pilate, G., Guiney, E., Holt, K., Petit-Conil, M., Lapierre, C., Leplé, J-C., ... Halpin, C. (2002). Field and pulping performances of transgenic trees with altered lignification. Nature Biotechnology, 20(6), 607-612. https://doi.org/10.1038/nbt0602-607
Pilate, Gilles ; Guiney, Emma ; Holt, Karen ; Petit-Conil, Michel ; Lapierre, Catherine ; Leplé, Jean-Charles ; Pollet, Brigitte ; Mila, Isabelle ; Webster, Elizabeth A ; Marstorp, Håkan G ; Hopkins, David W ; Jouanin, Lise ; Boerjan, Wout ; Schuch, Wolfgang ; Cornu, Daniel ; Halpin, Claire. / Field and pulping performances of transgenic trees with altered lignification. In: Nature Biotechnology. 2002 ; Vol. 20, No. 6. pp. 607-612.
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abstract = "The agronomic and pulping performance of transgenic trees with altered lignin has been evaluated in duplicated, long-term field trials. Poplars expressing cinnamyl alcohol dehydrogenase (CAD) or caffeate/5-hydroxy-ferulate O-methyltransferase (COMT) antisense transgenes were grown for four years at two sites, in France and England. The trees remained healthy throughout the trial. Growth indicators and interactions with insects were normal. No changes in soil microbial communities were detected beneath the transgenic trees. The expected modifications to lignin were maintained in the transgenics over four years, at both sites. Kraft pulping of tree trunks showed that the reduced-CAD lines had improved characteristics, allowing easier delignification, using smaller amounts of chemicals, while yielding more high-quality pulp. This work highlights the potential of engineering wood quality for more environmentally benign papermaking without interfering with tree growth or fitness.",
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Pilate, G, Guiney, E, Holt, K, Petit-Conil, M, Lapierre, C, Leplé, J-C, Pollet, B, Mila, I, Webster, EA, Marstorp, HG, Hopkins, DW, Jouanin, L, Boerjan, W, Schuch, W, Cornu, D & Halpin, C 2002, 'Field and pulping performances of transgenic trees with altered lignification', Nature Biotechnology, vol. 20, no. 6, pp. 607-612. https://doi.org/10.1038/nbt0602-607

Field and pulping performances of transgenic trees with altered lignification. / Pilate, Gilles; Guiney, Emma; Holt, Karen; Petit-Conil, Michel; Lapierre, Catherine; Leplé, Jean-Charles; Pollet, Brigitte; Mila, Isabelle; Webster, Elizabeth A; Marstorp, Håkan G; Hopkins, David W; Jouanin, Lise; Boerjan, Wout; Schuch, Wolfgang; Cornu, Daniel; Halpin, Claire.

In: Nature Biotechnology, Vol. 20, No. 6, 06.2002, p. 607-612.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Field and pulping performances of transgenic trees with altered lignification

AU - Pilate, Gilles

AU - Guiney, Emma

AU - Holt, Karen

AU - Petit-Conil, Michel

AU - Lapierre, Catherine

AU - Leplé, Jean-Charles

AU - Pollet, Brigitte

AU - Mila, Isabelle

AU - Webster, Elizabeth A

AU - Marstorp, Håkan G

AU - Hopkins, David W

AU - Jouanin, Lise

AU - Boerjan, Wout

AU - Schuch, Wolfgang

AU - Cornu, Daniel

AU - Halpin, Claire

PY - 2002/6

Y1 - 2002/6

N2 - The agronomic and pulping performance of transgenic trees with altered lignin has been evaluated in duplicated, long-term field trials. Poplars expressing cinnamyl alcohol dehydrogenase (CAD) or caffeate/5-hydroxy-ferulate O-methyltransferase (COMT) antisense transgenes were grown for four years at two sites, in France and England. The trees remained healthy throughout the trial. Growth indicators and interactions with insects were normal. No changes in soil microbial communities were detected beneath the transgenic trees. The expected modifications to lignin were maintained in the transgenics over four years, at both sites. Kraft pulping of tree trunks showed that the reduced-CAD lines had improved characteristics, allowing easier delignification, using smaller amounts of chemicals, while yielding more high-quality pulp. This work highlights the potential of engineering wood quality for more environmentally benign papermaking without interfering with tree growth or fitness.

AB - The agronomic and pulping performance of transgenic trees with altered lignin has been evaluated in duplicated, long-term field trials. Poplars expressing cinnamyl alcohol dehydrogenase (CAD) or caffeate/5-hydroxy-ferulate O-methyltransferase (COMT) antisense transgenes were grown for four years at two sites, in France and England. The trees remained healthy throughout the trial. Growth indicators and interactions with insects were normal. No changes in soil microbial communities were detected beneath the transgenic trees. The expected modifications to lignin were maintained in the transgenics over four years, at both sites. Kraft pulping of tree trunks showed that the reduced-CAD lines had improved characteristics, allowing easier delignification, using smaller amounts of chemicals, while yielding more high-quality pulp. This work highlights the potential of engineering wood quality for more environmentally benign papermaking without interfering with tree growth or fitness.

KW - Alcohol oxidoreductases

KW - Antisense elements

KW - Ecosystem

KW - Environmental pollution

KW - France

KW - Gene expression

KW - Industrial waste

KW - Lignin

KW - Methyltransferases

KW - Paper

KW - Soil microbiology

KW - Transgenes

KW - United Kingdom

KW - Wood

U2 - 10.1038/nbt0602-607

DO - 10.1038/nbt0602-607

M3 - Article

VL - 20

SP - 607

EP - 612

JO - Nature Biotechnology

JF - Nature Biotechnology

SN - 1087-0156

IS - 6

ER -

Pilate G, Guiney E, Holt K, Petit-Conil M, Lapierre C, Leplé J-C et al. Field and pulping performances of transgenic trees with altered lignification. Nature Biotechnology. 2002 Jun;20(6):607-612. https://doi.org/10.1038/nbt0602-607