Fine-mapping the wheat Snn1 locus conferring sensitivity 1 to the Parastagonospora nodorum necrotrophic effector SnTox1 using an eight founder multi-parent advanced generation intercross population

James Cockram, Alice Scuderi, Toby Barber, Eiko Furuki, Keith Gardner, Nick Gosman, Radoslaw Kowalczyk, Huyen Phan, Gemma A Rose, Kar-Chun Tan, Richard Oliver, Ian Mackay

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The necrotrophic fungus Parastagonospora nodorum is an important pathogen of one of the world’s most economically important cereal crops, wheat (Triticum aestivum L.). P. nodorum produces necrotrophic protein effectors that mediate host cell death, providing nutrients for continuation of the infection process. The recent discovery of pathogen effectors has revolutionized disease resistance breeding for necrotrophic diseases in crop species, allowing often complex genetic resistance mechanisms to be broken down into constituent parts. To date, three effectors have been identified in P. nodorum. Here we use the effector, SnTox1, to screen 642 progeny from an eight-parent multiparent advanced generation inter-cross (i.e., MAGIC) population, genotyped with a 90,000-feature single-nucleotide polymorphism array. The MAGIC founders showed a range of sensitivity to SnTox1, with transgressive segregation evident in the progeny. SnTox1 sensitivity showed high heritability, with quantitative trait locus analyses fine-mapping the Snn1 locus to the short arm of chromosome 1B. In addition, a previously undescribed SnTox1 sensitivity locus was identified on the long arm of chromosome 5A, termed here QSnn.niab-5A.1. The peak single-nucleotide polymorphism for the Snn1 locus was converted to the KASP genotyping platform, providing breeders and researchers a simple and cheap diagnostic marker for allelic state at Snn1.
Original languageEnglish
Pages (from-to)2257-2266
JournalG3: Genes, Genomes, Genetics
Volume5
Early online date24 Sep 2015
DOIs
Publication statusPrint publication - 1 Nov 2015
Externally publishedYes

Fingerprint

Triticum
Single Nucleotide Polymorphism
Chromosomes
single nucleotide polymorphism
loci
wheat
Disease Resistance
Quantitative Trait Loci
Population
Breeding
chromosomes
Fungi
Cell Death
transgressive segregation
pathogens
genetic resistance
Research Personnel
grain crops
resistance mechanisms
Food

Keywords

  • MAGIC
  • Plant disease
  • plant breeding

Cite this

Cockram, James ; Scuderi, Alice ; Barber, Toby ; Furuki, Eiko ; Gardner, Keith ; Gosman, Nick ; Kowalczyk, Radoslaw ; Phan, Huyen ; Rose, Gemma A ; Tan, Kar-Chun ; Oliver, Richard ; Mackay, Ian. / Fine-mapping the wheat Snn1 locus conferring sensitivity 1 to the Parastagonospora nodorum necrotrophic effector SnTox1 using an eight founder multi-parent advanced generation intercross population. In: G3: Genes, Genomes, Genetics. 2015 ; Vol. 5. pp. 2257-2266.
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abstract = "The necrotrophic fungus Parastagonospora nodorum is an important pathogen of one of the world’s most economically important cereal crops, wheat (Triticum aestivum L.). P. nodorum produces necrotrophic protein effectors that mediate host cell death, providing nutrients for continuation of the infection process. The recent discovery of pathogen effectors has revolutionized disease resistance breeding for necrotrophic diseases in crop species, allowing often complex genetic resistance mechanisms to be broken down into constituent parts. To date, three effectors have been identified in P. nodorum. Here we use the effector, SnTox1, to screen 642 progeny from an eight-parent multiparent advanced generation inter-cross (i.e., MAGIC) population, genotyped with a 90,000-feature single-nucleotide polymorphism array. The MAGIC founders showed a range of sensitivity to SnTox1, with transgressive segregation evident in the progeny. SnTox1 sensitivity showed high heritability, with quantitative trait locus analyses fine-mapping the Snn1 locus to the short arm of chromosome 1B. In addition, a previously undescribed SnTox1 sensitivity locus was identified on the long arm of chromosome 5A, termed here QSnn.niab-5A.1. The peak single-nucleotide polymorphism for the Snn1 locus was converted to the KASP genotyping platform, providing breeders and researchers a simple and cheap diagnostic marker for allelic state at Snn1.",
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Fine-mapping the wheat Snn1 locus conferring sensitivity 1 to the Parastagonospora nodorum necrotrophic effector SnTox1 using an eight founder multi-parent advanced generation intercross population. / Cockram, James; Scuderi, Alice; Barber, Toby; Furuki, Eiko; Gardner, Keith; Gosman, Nick; Kowalczyk, Radoslaw; Phan, Huyen; Rose, Gemma A; Tan, Kar-Chun; Oliver, Richard; Mackay, Ian.

In: G3: Genes, Genomes, Genetics, Vol. 5, 01.11.2015, p. 2257-2266.

Research output: Contribution to journalArticle

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AU - Cockram, James

AU - Scuderi, Alice

AU - Barber, Toby

AU - Furuki, Eiko

AU - Gardner, Keith

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AU - Kowalczyk, Radoslaw

AU - Phan, Huyen

AU - Rose, Gemma A

AU - Tan, Kar-Chun

AU - Oliver, Richard

AU - Mackay, Ian

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AB - The necrotrophic fungus Parastagonospora nodorum is an important pathogen of one of the world’s most economically important cereal crops, wheat (Triticum aestivum L.). P. nodorum produces necrotrophic protein effectors that mediate host cell death, providing nutrients for continuation of the infection process. The recent discovery of pathogen effectors has revolutionized disease resistance breeding for necrotrophic diseases in crop species, allowing often complex genetic resistance mechanisms to be broken down into constituent parts. To date, three effectors have been identified in P. nodorum. Here we use the effector, SnTox1, to screen 642 progeny from an eight-parent multiparent advanced generation inter-cross (i.e., MAGIC) population, genotyped with a 90,000-feature single-nucleotide polymorphism array. The MAGIC founders showed a range of sensitivity to SnTox1, with transgressive segregation evident in the progeny. SnTox1 sensitivity showed high heritability, with quantitative trait locus analyses fine-mapping the Snn1 locus to the short arm of chromosome 1B. In addition, a previously undescribed SnTox1 sensitivity locus was identified on the long arm of chromosome 5A, termed here QSnn.niab-5A.1. The peak single-nucleotide polymorphism for the Snn1 locus was converted to the KASP genotyping platform, providing breeders and researchers a simple and cheap diagnostic marker for allelic state at Snn1.

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