Fleas of fleas: The potential role of bacteriophages in Salmonella diversity and pathogenicity.

Kirstie Andrews, Toby Landeryou, JYN Nale*

*Corresponding author for this work

Research output: Contribution to conferencePosterpeer-review

132 Downloads (Pure)

Abstract

Non-typhoidal salmonellosis is an important foodborne and zoonotic infection, that causes significant global public health concern. Diverse serovars are multidrug-resistant and encode several virulence indicators, however, little is known on the role prophages play in driving these characteristics. Here, we extracted prophages from 75 Salmonella genomes, which represent the 15 most important serovars in the United Kingdom. We analysed the genomes of the intact prophages for the presence of virulence factors which were associated with; diversity, evolution and pathogenicity of Salmonella and to establish their genomic relationships. We identified 615 prophage elements from the Salmonella genomes, from which 195 prophages are intact, 332 being incomplete while 88 are questionable. The average prophage carriage was found to be more prevalent in S. Heidelberg, S. Inverness and S. Newport (10.2-11.6 prophages/strain), compared to S. Infantis, S. Stanley, S. Typhimurium and S. Virchow (8.2-9 prophages/strain) and S. Agona, S. Braenderup, S. Bovismorbificans, S. Choleraesuis, S. Dublin, and S. Java (6-7.8 prophages/strain), and S. Javiana and S. Enteritidis (5.8 prophages/strain). Cumulatively, 2760 virulence factors were detected from the intact prophages and associated with cellular functionality being linked to effector delivery/secretion system (73%), adherence (22%), magnesium uptake (2.7%), resistance to antimicrobial peptides (0.94%), stress/survival (0.4%), exotoxins (0.32%) and antivirulence (0.18%). Close and distant clusters were formed among the prophage genomes suggesting different lineages and associations with bacteriophages of other Enterobacteriaceae. We show that diverse repertoire of Salmonella prophages are associated with numerous virulence factors, and may contribute to diversity, pathogenicity and success of specific serovars.
Original languageEnglish
Pages351
Number of pages1
Publication statusPrint publication - Apr 2024
EventMicrobiology Society Annual Conference 2024 - Edinburgh International Convention Centre., Edinburgh, United Kingdom
Duration: 8 Apr 202411 Apr 2024
https://microbiologysociety.org/event/annual-conference/annual-conference-2024.html#tab-0

Conference

ConferenceMicrobiology Society Annual Conference 2024
Country/TerritoryUnited Kingdom
CityEdinburgh
Period8/04/2411/04/24
Internet address

Fingerprint

Dive into the research topics of 'Fleas of fleas: The potential role of bacteriophages in Salmonella diversity and pathogenicity.'. Together they form a unique fingerprint.

Cite this