Framework for quantification of the dynamics of root colonization by Pseudomonas fluorescens isolate SBW25

Daire Carroll, NH Holden, Miriam Gifford, Lionel Dupuy*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)
4 Downloads (Pure)

Abstract

Colonization of the root surface, or rhizoplane, is one of the first steps for soil-borne bacteria to become established in the plant microbiome. However, the relative contributions of processes, such as bacterial attachment and proliferation is not well characterized, and this limits our ability to comprehend the complex dynamics of microbial communities in the rhizosphere. The work presented here addresses this knowledge gap. A model system was developed to acquire quantitative data on the colonization process of lettuce (Lactuca sativa L. cultivar. All Year Round) roots by Pseudomonas fluorsecens isolate SBW25. A
theoretical framework is proposed to calculate attachment rate and quantify the relative contribution of bacterial attachment to colonization. This allows the assessment of attachment rates on the root surface beyond the short time period during which it can be quantified experimentally. All techniques proposed are generic and similar analyses could be applied to study various combinations of plants and bacteria, or to assess competition between species. In the future this could allow for selection of microbial traits that improve early colonization and maintenance of targeted isolates in cropping systems, with potential applications for the development of biological fertilizers.
Original languageEnglish
Article number585443
JournalFrontiers in Microbiology
Volume11
Early online date25 Sep 2020
DOIs
Publication statusFirst published - 25 Sep 2020

Keywords

  • Pseudomonas fluorescens
  • attachment
  • bacterial dynamics
  • colonization
  • microbiome
  • rhizosphere
  • root surface

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