Functional Analysis of Shiga Toxin-Producing Escherichia coli Biofilm Components in Plant Leaves

Nicola J Holden, Kathryn M Wright, Jacqueline Marshall, Ashleigh Holmes

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

Plants represent alternative or secondary hosts for Shiga toxin-producing Escherichia coli (STEC), enabling transmission of the pathogens through the food chain on horticultural crops. This becomes a public health concern for plants that are eaten raw or minimally processed, such as leafy salad and fruits. STEC actively interact with plants as hosts, and so to determine the mechanistic basis to the interaction, it is necessary to assess STEC gene function in planta. Here, we describe analysis of an STEC biofilm component, curli, that plays a role in STEC colony formation in plant leaves. It also serves as a suitable example of the approaches required for qualitative and quantitative assessment of functional host colonization traits.
Original languageEnglish
Title of host publicationShiga Toxin-Producing E. coli
EditorsStephanie Schueller, Martina Bielaszewska
Place of PublicationNew York
PublisherSpringer Science & Business Media
Pages163-175
Number of pages13
Volume2291
ISBN (Electronic)978-1-0716-1339-9
ISBN (Print)978-1-0716-1338-2
DOIs
Publication statusFirst published - 12 Mar 2021

Publication series

NameMethods in Molecular Biology
Volume2291
ISSN (Print)1064-3745
ISSN (Electronic)1940-6029

Keywords

  • Adhesins
  • Biofilms
  • E. coli O157:H7
  • Gene expression
  • Microscopy
  • Vegetables

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