Functional Analysis of Shiga Toxin-Producing Escherichia coli Biofilm Components in Plant Leaves

Nicola J Holden; Kathryn M Wright; Jacqueline Marshall; Ashleigh Holmes

doi:10.1007/978-1-0716-1339-9_7

Functional Analysis of Shiga Toxin-Producing Escherichia coli Biofilm Components in Plant Leaves

Nicola J Holden, Kathryn M Wright, Jacqueline Marshall, Ashleigh Holmes

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

1 Citation (Scopus)

Abstract

Plants represent alternative or secondary hosts for Shiga toxin-producing Escherichia coli (STEC), enabling transmission of the pathogens through the food chain on horticultural crops. This becomes a public health concern for plants that are eaten raw or minimally processed, such as leafy salad and fruits. STEC actively interact with plants as hosts, and so to determine the mechanistic basis to the interaction, it is necessary to assess STEC gene function in planta. Here, we describe analysis of an STEC biofilm component, curli, that plays a role in STEC colony formation in plant leaves. It also serves as a suitable example of the approaches required for qualitative and quantitative assessment of functional host colonization traits.

Original language	English
Title of host publication	Shiga Toxin-Producing E. coli
Editors	Stephanie Schueller, Martina Bielaszewska
Place of Publication	New York
Publisher	Springer Science & Business Media
Pages	163-175
Number of pages	13
Volume	2291
ISBN (Electronic)	978-1-0716-1339-9
ISBN (Print)	978-1-0716-1338-2
DOIs	https://doi.org/10.1007/978-1-0716-1339-9_7
Publication status	First published - 12 Mar 2021

Publication series

Name	Methods in Molecular Biology
Volume	2291
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Keywords

Adhesins
Biofilms
E. coli O157:H7
Gene expression
Microscopy
Vegetables

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/978-1-0716-1339-9_7

Cite this

Holden, N. J., Wright, K. M., Marshall, J., & Holmes, A. (2021). Functional Analysis of Shiga Toxin-Producing Escherichia coli Biofilm Components in Plant Leaves. In S. Schueller, & M. Bielaszewska (Eds.), Shiga Toxin-Producing E. coli (Vol. 2291, pp. 163-175). (Methods in Molecular Biology; Vol. 2291). Springer Science & Business Media. Advance online publication. https://doi.org/10.1007/978-1-0716-1339-9_7

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Holden, NJ, Wright, KM, Marshall, J & Holmes, A 2021, Functional Analysis of Shiga Toxin-Producing Escherichia coli Biofilm Components in Plant Leaves. in S Schueller & M Bielaszewska (eds), Shiga Toxin-Producing E. coli. vol. 2291, Methods in Molecular Biology, vol. 2291, Springer Science & Business Media, New York, pp. 163-175. https://doi.org/10.1007/978-1-0716-1339-9_7

Functional Analysis of Shiga Toxin-Producing Escherichia coli Biofilm Components in Plant Leaves. / Holden, Nicola J; Wright, Kathryn M; Marshall, Jacqueline et al.
Shiga Toxin-Producing E. coli. ed. / Stephanie Schueller; Martina Bielaszewska. Vol. 2291 New York: Springer Science & Business Media, 2021. p. 163-175 (Methods in Molecular Biology; Vol. 2291).

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

TY - CHAP

T1 - Functional Analysis of Shiga Toxin-Producing Escherichia coli Biofilm Components in Plant Leaves

AU - Holden, Nicola J

AU - Wright, Kathryn M

AU - Marshall, Jacqueline

AU - Holmes, Ashleigh

PY - 2021/3/12

Y1 - 2021/3/12

N2 - Plants represent alternative or secondary hosts for Shiga toxin-producing Escherichia coli (STEC), enabling transmission of the pathogens through the food chain on horticultural crops. This becomes a public health concern for plants that are eaten raw or minimally processed, such as leafy salad and fruits. STEC actively interact with plants as hosts, and so to determine the mechanistic basis to the interaction, it is necessary to assess STEC gene function in planta. Here, we describe analysis of an STEC biofilm component, curli, that plays a role in STEC colony formation in plant leaves. It also serves as a suitable example of the approaches required for qualitative and quantitative assessment of functional host colonization traits.

AB - Plants represent alternative or secondary hosts for Shiga toxin-producing Escherichia coli (STEC), enabling transmission of the pathogens through the food chain on horticultural crops. This becomes a public health concern for plants that are eaten raw or minimally processed, such as leafy salad and fruits. STEC actively interact with plants as hosts, and so to determine the mechanistic basis to the interaction, it is necessary to assess STEC gene function in planta. Here, we describe analysis of an STEC biofilm component, curli, that plays a role in STEC colony formation in plant leaves. It also serves as a suitable example of the approaches required for qualitative and quantitative assessment of functional host colonization traits.

KW - Adhesins

KW - Biofilms

KW - E. coli O157:H7

KW - Gene expression

KW - Microscopy

KW - Vegetables

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DO - 10.1007/978-1-0716-1339-9_7

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SN - 978-1-0716-1338-2

VL - 2291

T3 - Methods in Molecular Biology

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BT - Shiga Toxin-Producing E. coli

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Holden NJ, Wright KM, Marshall J, Holmes A. Functional Analysis of Shiga Toxin-Producing Escherichia coli Biofilm Components in Plant Leaves. In Schueller S, Bielaszewska M, editors, Shiga Toxin-Producing E. coli. Vol. 2291. New York: Springer Science & Business Media. 2021. p. 163-175. (Methods in Molecular Biology). Epub 2021 Mar 12. doi: 10.1007/978-1-0716-1339-9_7

Functional Analysis of Shiga Toxin-Producing Escherichia coli Biofilm Components in Plant Leaves

Abstract

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Keywords

UN SDGs

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