Fungal networks and orchid distribution: New insights from above- and below-ground analyses of fungal communities

Lorenzo Pecoraro*, Tancredi Caruso, Lei Cai, Vijai Kumar Gupta, Zhong Jian Liu

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)

Abstract

Orchids are critically dependent on fungi for seedling establishment and growth, so the distribution and diversity of orchids might depend on the associated fungal communities. We characterised the communities associated with eight orchid species in three Mediterranean protected areas, using a combination of above-ground analyses of sporophores and below-ground molecular analyses of orchid root samples. In three years of sporophore collection in 25 plots around flowering orchid plants, 268 macrofungal species belonging to 84 genera were observed. Statistical analyses indicated a correlation between macrofungal diversity and orchid community variation, regardless of the effect of environmental and spatial factors characterizing the investigated orchid sites. Fungal ITS-DNA PCR amplification, cloning, and sequencing revealed Rhizoctonia-like fungi belonging to Ceratobasidiaceae (26 %), Tulasnellaceae (22.5 %), and Sebacinaceae (3.5 %), as well as other basidiomycetes and ascomycetes, in the roots of 99 orchid plants. Mycorrhizal specificity was low but co-occurring orchid species showed preferences for diffeent partners. The diverse macrofungal communities found in the sites may contribute to orchid community variation without colonizing the orchid roots. Molecular analyses revealed a segregation of associated fungi, which may contribute to Mediterranean orchid coexistence in nature.

Original languageEnglish
Pages (from-to)45-66
Number of pages22
JournalIMA Fungus
Volume9
Issue number1
Early online date12 Feb 2018
DOIs
Publication statusPrint publication - Jun 2018
Externally publishedYes

Keywords

  • Endophytic fungi
  • Macrofungal communities
  • Mediterranean
  • Molecular ecology
  • Mycorrhizas
  • Orchid-fungus relationships
  • Spatial distributionSpecies coexistence

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