Genome-wide association of yield traits in a nested association mapping population of barley reveals new gene diversity for future breeding

Rajiv Sharma, Fulvia Draicchio, Hazel Bull, Paul Herzig, Andreas Maurer, Klaus Pillen, William T B Thomas, Andrew J Flavell

Research output: Contribution to journalArticleResearchpeer-review

7 Citations (Scopus)

Abstract

To explore wild barley as a source of useful alleles for yield improvement in breeding, we have carried out a genome-wide association scan using the nested association mapping population HEB-25, which contains 25 diverse exotic barley genomes superimposed on an ~70% genetic background of cultivated barley. A total of 1420 HEB-25 lines were trialled for nine yield-related grain traits for 2 years in Germany and Scotland, with varying N fertilizer application. The phenotypic data were related to genotype scores for 5398 gene-based single nucleotide polymorphism (SNP) markers. A total of 96 quantitative trait locus (QTL) regions were identified across all measured traits, the majority of which co-localize with known major genes controlling flowering time (Ppd-H2, HvCEN, HvGI, VRN-H1, and VRN-H3) and spike morphology (VRS3, VRS1, VRS4, and INT-C) in barley. Fourteen QTL hotspots, with at least three traits coinciding, were also identified, several of which co-localize with barley orthologues of genes controlling grain dimensions in rice. Most of the allele effects are specific to geographical location and/or exotic parental genotype. This study shows the existence of beneficial alleles for yield-related traits in exotic barley germplasm and provides candidate alleles for future improvement of these traits by the breeder.

Original languageEnglish
Pages (from-to)3811-3822
Number of pages12
JournalJournal of Experimental Botany
Volume69
Issue number16
DOIs
Publication statusPrint publication - 20 Jul 2018
Externally publishedYes

Fingerprint

Hordeum
chromosome mapping
Breeding
barley
Genome
genome
breeding
Alleles
Population
Genes
alleles
genes
Quantitative Trait Loci
quantitative trait loci
Genotype
genotype
Genome-Wide Association Study
Fertilizers
Geographical Locations
Scotland

Cite this

Sharma, Rajiv ; Draicchio, Fulvia ; Bull, Hazel ; Herzig, Paul ; Maurer, Andreas ; Pillen, Klaus ; Thomas, William T B ; Flavell, Andrew J. / Genome-wide association of yield traits in a nested association mapping population of barley reveals new gene diversity for future breeding. In: Journal of Experimental Botany. 2018 ; Vol. 69, No. 16. pp. 3811-3822.
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Genome-wide association of yield traits in a nested association mapping population of barley reveals new gene diversity for future breeding. / Sharma, Rajiv; Draicchio, Fulvia; Bull, Hazel; Herzig, Paul; Maurer, Andreas; Pillen, Klaus; Thomas, William T B; Flavell, Andrew J.

In: Journal of Experimental Botany, Vol. 69, No. 16, 20.07.2018, p. 3811-3822.

Research output: Contribution to journalArticleResearchpeer-review

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