Genotyping SSR length variants by isothermal DNA amplification

David Lee; Manuela Ialicicco; Harika Akkinepalli; Giacomo Morreale; Yan Liu; Hei Leung; Gabriella S. Scippa; Andy Greenland; Ian Mackay

doi:10.1139/g2012-058

Genotyping SSR length variants by isothermal DNA amplification

David Lee, Manuela Ialicicco, Harika Akkinepalli, Giacomo Morreale, Yan Liu, Hei Leung, Gabriella S. Scippa, Andy Greenland, Ian Mackay

Research output: Contribution to journal › Article › peer-review

Abstract

Loop-mediated isothermal DNA amplification (LAMP) is an alternative method for the amplification of DNA sequences. It has been applied primarily for the detection of specific targets. We demonstrate the novel use of LAMP to amplify SSR alleles in a set of rice varieties and show the results to be consistent with analysis performed by PCR. Furthermore, we test the sensitivity of the assay and show it to amplify from near single copy target.

Original language	English
Pages (from-to)	691-695
Number of pages	5
Journal	Genome
Volume	55
Issue number	09
DOIs	https://doi.org/10.1139/g2012-058
Publication status	Print publication - 17 Oct 2012
Externally published	Yes

Keywords

Rice microsatellite
LAMP
PCR
Sensitivity

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10.1139/g2012-058

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abstract = "Loop-mediated isothermal DNA amplification (LAMP) is an alternative method for the amplification of DNA sequences. It has been applied primarily for the detection of specific targets. We demonstrate the novel use of LAMP to amplify SSR alleles in a set of rice varieties and show the results to be consistent with analysis performed by PCR. Furthermore, we test the sensitivity of the assay and show it to amplify from near single copy target.",

keywords = "Rice microsatellite, LAMP, PCR, Sensitivity",

author = "David Lee and Manuela Ialicicco and Harika Akkinepalli and Giacomo Morreale and Yan Liu and Hei Leung and Scippa, {Gabriella S.} and Andy Greenland and Ian Mackay",

year = "2012",

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T1 - Genotyping SSR length variants by isothermal DNA amplification

AU - Lee, David

AU - Ialicicco, Manuela

AU - Akkinepalli, Harika

AU - Morreale, Giacomo

AU - Liu, Yan

AU - Leung, Hei

AU - Scippa, Gabriella S.

AU - Greenland, Andy

AU - Mackay, Ian

PY - 2012/10/17

Y1 - 2012/10/17

N2 - Loop-mediated isothermal DNA amplification (LAMP) is an alternative method for the amplification of DNA sequences. It has been applied primarily for the detection of specific targets. We demonstrate the novel use of LAMP to amplify SSR alleles in a set of rice varieties and show the results to be consistent with analysis performed by PCR. Furthermore, we test the sensitivity of the assay and show it to amplify from near single copy target.

AB - Loop-mediated isothermal DNA amplification (LAMP) is an alternative method for the amplification of DNA sequences. It has been applied primarily for the detection of specific targets. We demonstrate the novel use of LAMP to amplify SSR alleles in a set of rice varieties and show the results to be consistent with analysis performed by PCR. Furthermore, we test the sensitivity of the assay and show it to amplify from near single copy target.

KW - Rice microsatellite

KW - LAMP

KW - PCR

KW - Sensitivity

UR - http://www.mendeley.com/research/genotyping-ssr-length-variants-isothermal-dna-amplification-1

U2 - 10.1139/g2012-058

DO - 10.1139/g2012-058

M3 - Article

SN - 1474-7596

VL - 55

SP - 691

EP - 695

JO - Genome

JF - Genome

IS - 09

ER -

Genotyping SSR length variants by isothermal DNA amplification

Abstract

Keywords

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