Genotyping SSR length variants by isothermal DNA amplification

David Lee, Manuela Ialicicco, Harika Akkinepalli, Giacomo Morreale, Yan Liu, Hei Leung, Gabriella S. Scippa, Andy Greenland, Ian Mackay

Research output: Contribution to journalArticle

Abstract

Loop-mediated isothermal DNA amplification (LAMP) is an alternative method for the amplification of DNA sequences. It has been applied primarily for the detection of specific targets. We demonstrate the novel use of LAMP to amplify SSR alleles in a set of rice varieties and show the results to be consistent with analysis performed by PCR. Furthermore, we test the sensitivity of the assay and show it to amplify from near single copy target.
Original languageEnglish
Pages (from-to)691-695
Number of pages5
JournalGenome
Volume55
Issue number09
DOIs
Publication statusPrint publication - 17 Oct 2012
Externally publishedYes

Fingerprint

genotyping
amplification
Alleles
alleles
nucleotide sequences
DNA
rice
Polymerase Chain Reaction
assays
allele
testing
assay
methodology
Oryza
analysis
method
test
detection

Keywords

  • Rice microsatellite
  • LAMP
  • PCR
  • Sensitivity

Cite this

Lee, D., Ialicicco, M., Akkinepalli, H., Morreale, G., Liu, Y., Leung, H., ... Mackay, I. (2012). Genotyping SSR length variants by isothermal DNA amplification. Genome, 55(09), 691-695. https://doi.org/10.1139/g2012-058
Lee, David ; Ialicicco, Manuela ; Akkinepalli, Harika ; Morreale, Giacomo ; Liu, Yan ; Leung, Hei ; Scippa, Gabriella S. ; Greenland, Andy ; Mackay, Ian. / Genotyping SSR length variants by isothermal DNA amplification. In: Genome. 2012 ; Vol. 55, No. 09. pp. 691-695.
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keywords = "Rice microsatellite, LAMP, PCR, Sensitivity",
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Lee, D, Ialicicco, M, Akkinepalli, H, Morreale, G, Liu, Y, Leung, H, Scippa, GS, Greenland, A & Mackay, I 2012, 'Genotyping SSR length variants by isothermal DNA amplification', Genome, vol. 55, no. 09, pp. 691-695. https://doi.org/10.1139/g2012-058

Genotyping SSR length variants by isothermal DNA amplification. / Lee, David; Ialicicco, Manuela; Akkinepalli, Harika; Morreale, Giacomo; Liu, Yan; Leung, Hei; Scippa, Gabriella S.; Greenland, Andy; Mackay, Ian.

In: Genome, Vol. 55, No. 09, 17.10.2012, p. 691-695.

Research output: Contribution to journalArticle

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T1 - Genotyping SSR length variants by isothermal DNA amplification

AU - Lee, David

AU - Ialicicco, Manuela

AU - Akkinepalli, Harika

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AU - Liu, Yan

AU - Leung, Hei

AU - Scippa, Gabriella S.

AU - Greenland, Andy

AU - Mackay, Ian

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Lee D, Ialicicco M, Akkinepalli H, Morreale G, Liu Y, Leung H et al. Genotyping SSR length variants by isothermal DNA amplification. Genome. 2012 Oct 17;55(09):691-695. https://doi.org/10.1139/g2012-058