Genotyping SSR length variants by isothermal DNA amplification

David Lee, Manuela Ialicicco, Harika Akkinepalli, Giacomo Morreale, Yan Liu, Hei Leung, Gabriella S. Scippa, Andy Greenland, Ian Mackay

Research output: Contribution to journalArticle

Abstract

Loop-mediated isothermal DNA amplification (LAMP) is an alternative method for the amplification of DNA sequences. It has been applied primarily for the detection of specific targets. We demonstrate the novel use of LAMP to amplify SSR alleles in a set of rice varieties and show the results to be consistent with analysis performed by PCR. Furthermore, we test the sensitivity of the assay and show it to amplify from near single copy target.
Original languageEnglish
Pages (from-to)691-695
Number of pages5
JournalGenome
Volume55
Issue number09
DOIs
Publication statusPrint publication - 17 Oct 2012
Externally publishedYes

Keywords

  • Rice microsatellite
  • LAMP
  • PCR
  • Sensitivity

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  • Cite this

    Lee, D., Ialicicco, M., Akkinepalli, H., Morreale, G., Liu, Y., Leung, H., Scippa, G. S., Greenland, A., & Mackay, I. (2012). Genotyping SSR length variants by isothermal DNA amplification. Genome, 55(09), 691-695. https://doi.org/10.1139/g2012-058