Global transcriptomic profiles of circulating leucocytes in early lactation cows with clinical or subclinical mastitis

Zhangrui Cheng*, Laura Buggiotti, Mazdak Salavati, Cinzia Marchitelli, Sergio Palma-Vera, Alistair Wylie, Haruko Takeda, Lijing Tang, Mark Crowe, Claire Wathes, GplusE Consortium

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

15 Citations (Scopus)

Abstract

Bovine mastitis, an inflammatory disease of the mammary gland, is classified as subclinical or clinical. Circulating neutrophils are recruited to the udder to combat infection. We compared the transcriptomic profiles in circulating leukocytes between healthy cows and those with naturally occurring subclinical or clinical mastitis. Holstein Friesian dairy cows from six farms in EU countries were recruited. Based on milk somatic cell count and clinical records, cows were classified as healthy (n = 147), subclinically (n = 45) or clinically mastitic (n = 22). Circulating leukocyte RNA was sequenced with Illumina NextSeq single end reads (30 M). Differentially expressed genes (DEGs) between the groups were identified using CLC Genomics Workbench V21, followed by GO enrichment analysis. Both subclinical and clinical mastitis caused significant changes in the leukocyte transcriptome, with more intensive changes attributed to clinical mastitis. We detected 769 DEGs between clinical and healthy groups, 258 DEGs between subclinical and healthy groups and 193 DEGs between clinical and subclinical groups. Most DEGs were associated with cell killing and immune processes. Many upregulated DEGs in clinical mastitis encoded antimicrobial peptides (AZU1, BCL3, CAMP, CATHL1, CATHL2, CATHL4,CATHL5, CATHL6, CCL1, CXCL2, CXCL13, DEFB1, DEFB10, DEFB4A, DEFB7, LCN2, PGLYRP1, PRTN3, PTX3, S100A8, S100A9, S100A12, SLC11A1, TF and LTF) which were not upregulated in subclinical mastitis. The use of transcriptomic profiles has identified a much greater up-regulation of genes encoding antimicrobial peptides in circulating leukocytes of cows with naturally occurring clinical compared with subclinical mastitis. These could play a key role in combatting disease organisms.

Original languageEnglish
Pages (from-to)4611-4623
Number of pages13
JournalMolecular Biology Reports
Volume48
Issue number5
DOIs
Publication statusPrint publication - May 2021
Externally publishedYes

Bibliographical note

© 2021, The Author(s), under exclusive licence to Springer Nature B.V.

Keywords

  • Bovine mastitis
  • Inflammation
  • Next generation sequence
  • Transcriptome

Fingerprint

Dive into the research topics of 'Global transcriptomic profiles of circulating leucocytes in early lactation cows with clinical or subclinical mastitis'. Together they form a unique fingerprint.

Cite this