GWAS scans of cereal cyst nematode (Heterodera avenae) resistance in Indian wheat germplasm

Vikas Kumar Singh, Deepti Chaturvedi, Saksham Pundir, Deepak Kumar, Rajiv Sharma, Sundeep Kumar, Shiveta Sharma, Shailendra Sharma

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

Significant yield losses in major cereal-growing regions around the world have been linked to cereal cyst nematodes (Heterodera spp.). Identifying and deploying natural sources of resistance is of utmost importance due to increasing concerns associated with chemical methods over the years. We screened 141 diverse wheat genotypes collected from pan-Indian wheat cultivation states for nematode resistance over two years, alongside two resistant (Raj MR1, W7984 (M6)) and two susceptible (WH147, Opata M85) checks. We performed genome-wide association analysis using four single-locus models (GLM, MLM, CMLM, and ECMLM) and three multi-locus models (Blink, FarmCPU, and MLMM). Single locus models identified nine significant MTAs (−log10 (P) > 3.0) on chromosomes 2A, 3B, and 4B whereas, multi-locus models identified 11 significant MTAs on chromosomes 1B, 2A, 3B, 3D and 4B. Single and multi-locus models identified nine common significant MTAs. Candidate gene analysis identified 33 genes like F-box-like domain superfamily, Cytochrome P450 superfamily, Leucine-rich repeat, cysteine-containing subtype Zinc finger RING/FYVE/PHD-type, etc., having a putative role in disease resistance. Such genetic resources can help to reduce the impact of this disease on wheat production. Additionally, these results can be used to design new strategies for controlling the spread of H. avenae, such as the development of resistant varieties or the use of resistant cultivars. Finally, the obtained results can also be used to identify new sources of resistance to this pathogen and develop novel control methods.

Original languageEnglish
Pages (from-to)579-601
Number of pages23
JournalMolecular Genetics and Genomics
Volume298
Issue number3
Early online date8 Mar 2023
DOIs
Publication statusPrint publication - May 2023

Bibliographical note

© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.

Keywords

  • Heterodera avenae
  • Triticum aestivum
  • GWAS
  • Cereal cyst nematode
  • Resistance
  • Genome-Wide Association Study
  • Animals
  • Cysts
  • Tylenchoidea/genetics
  • Triticum/genetics
  • Edible Grain/genetics

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