Problem The type I interferon (IFN), IFN-tau (τ), is the primary embryonic signal for pregnancy maintenance in ruminants. This study determined the effects of heat shock upon IFN-τ (IFNT) gene expression by bovine blastocysts in vitro. Method of study In vitro-produced blastocyst-stage embryos were exposed to 42°C for 4 hr, and mRNA for heat-shock protein 70 (HSP70) and IFNT quantified. Results Heat shock increased both HSP70 and IFNT expression. There was a significant correlation between HSP70 and IFNT transcript levels irrespective of whether a blastocyst had been exposed to heat shock or not. Conclusion The increase in IFNT as a result of heat shock suggests that a proportion of the variation in IFNT expression observed in blastocyst-stage embryos is a response to stress.
- Heat shock protein 70
- In-vitro production
Hickman, CF., Clinton, M., Ainslie, A., Ashworth, CJ., & Rooke, JA. (2013). Heat shock induces interferon-TAU gene expression by in vitro-produced bovine blastocysts. American Journal of Reproductive Immunology, 70(3), 177 - 181. https://doi.org/10.1111/aji.12131