Host-specific differences in the response of cultured macrophages to Campylobacter jejuni capsule and O-methyl phosphoramidate mutants

S Kim, A Vela, SM Clohisey, S Athanasiadou, P Kaiser, MP Stevens, L Vervelde

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Campylobacter jejuni is the leading cause of bacterial food-borne gastroenteritis worldwide and human infections are frequently associated with handling and consumption of contaminated poultry. The polysaccharide capsule of C. jejuni plays important roles in colonisation of the chicken gut, invasion of epithelial cells and serum resistance and is subject to modification with O-methyl phosphoramidate (MeOPN) in most strains. In this study, the cytokine responses of mouse bone marrow-derived macrophages (mBMMs), chicken bone marrow-derived macrophages (chBMMs) and human monocyte-derived macrophages (hMDMs) were measured following infection with C. jejuni 11168H wild-type (WT) or isogenic mutants lacking either the capsule (Δcj1439) or its MeOPN modification (Δcj1417). Consistent with previous observations using murine bone marrow-derived dendritic cells, mutants lacking the capsule or MeOPN elicited enhanced transcription of IL-6 and IL-10 in mBMMs compared to wild-type C. jejuni. However, the lack of capsule and MeOPN did not alter IL-6 and IL-10 expression in chBMMs and hMDMs compared to C. jejuni WT. Phagocytosis assays showed the acapsular mutant was not impaired in uptake or net intracellular survival after phagocytosis in both chicken and human macrophages; however, the phagocytosis of the MeOPN mutant was significantly decreased in both chicken and human macrophages. In conclusion, differences in the response of macrophages of varying host origin to Campylobacter were detected. The absence of MeOPN modification on the capsule of C. jejuni did not alter the levels of innate cytokine expression in both chicken and human macrophages compared to the 11168H WT, but affected phagocytosis by host macrophages.
Original languageEnglish
Article number3
Number of pages10
JournalVeterinary Research
Volume49
Early online date9 Jan 2018
DOIs
Publication statusFirst published - 9 Jan 2018

Fingerprint

Campylobacter jejuni
Capsules
Macrophages
Chickens
Phagocytosis
Interleukin-10
Interleukin-6
phosphoramidic acid
Cytokines
Campylobacter
Gastroenteritis
Poultry
Infection
Dendritic Cells
Polysaccharides
Bone Marrow
Epithelial Cells

Bibliographical note

1031399

Keywords

  • Amides/metabolism
  • Animals
  • Bacterial Capsules/metabolism
  • Bone Marrow
  • Campylobacter Infections/microbiology
  • Campylobacter jejuni/genetics
  • Chickens
  • Cytokines/metabolism
  • Humans
  • Macrophages/metabolism
  • Monocytes/metabolism
  • Mutation
  • Phosphoric Acids/metabolism
  • Poultry Diseases/microbiology
  • Rats

Cite this

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abstract = "Campylobacter jejuni is the leading cause of bacterial food-borne gastroenteritis worldwide and human infections are frequently associated with handling and consumption of contaminated poultry. The polysaccharide capsule of C. jejuni plays important roles in colonisation of the chicken gut, invasion of epithelial cells and serum resistance and is subject to modification with O-methyl phosphoramidate (MeOPN) in most strains. In this study, the cytokine responses of mouse bone marrow-derived macrophages (mBMMs), chicken bone marrow-derived macrophages (chBMMs) and human monocyte-derived macrophages (hMDMs) were measured following infection with C. jejuni 11168H wild-type (WT) or isogenic mutants lacking either the capsule (Δcj1439) or its MeOPN modification (Δcj1417). Consistent with previous observations using murine bone marrow-derived dendritic cells, mutants lacking the capsule or MeOPN elicited enhanced transcription of IL-6 and IL-10 in mBMMs compared to wild-type C. jejuni. However, the lack of capsule and MeOPN did not alter IL-6 and IL-10 expression in chBMMs and hMDMs compared to C. jejuni WT. Phagocytosis assays showed the acapsular mutant was not impaired in uptake or net intracellular survival after phagocytosis in both chicken and human macrophages; however, the phagocytosis of the MeOPN mutant was significantly decreased in both chicken and human macrophages. In conclusion, differences in the response of macrophages of varying host origin to Campylobacter were detected. The absence of MeOPN modification on the capsule of C. jejuni did not alter the levels of innate cytokine expression in both chicken and human macrophages compared to the 11168H WT, but affected phagocytosis by host macrophages.",
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Host-specific differences in the response of cultured macrophages to Campylobacter jejuni capsule and O-methyl phosphoramidate mutants. / Kim, S; Vela, A; Clohisey, SM; Athanasiadou, S; Kaiser, P; Stevens, MP; Vervelde, L.

In: Veterinary Research, Vol. 49, 3, 09.01.2018.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Host-specific differences in the response of cultured macrophages to Campylobacter jejuni capsule and O-methyl phosphoramidate mutants

AU - Kim, S

AU - Vela, A

AU - Clohisey, SM

AU - Athanasiadou, S

AU - Kaiser, P

AU - Stevens, MP

AU - Vervelde, L

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AB - Campylobacter jejuni is the leading cause of bacterial food-borne gastroenteritis worldwide and human infections are frequently associated with handling and consumption of contaminated poultry. The polysaccharide capsule of C. jejuni plays important roles in colonisation of the chicken gut, invasion of epithelial cells and serum resistance and is subject to modification with O-methyl phosphoramidate (MeOPN) in most strains. In this study, the cytokine responses of mouse bone marrow-derived macrophages (mBMMs), chicken bone marrow-derived macrophages (chBMMs) and human monocyte-derived macrophages (hMDMs) were measured following infection with C. jejuni 11168H wild-type (WT) or isogenic mutants lacking either the capsule (Δcj1439) or its MeOPN modification (Δcj1417). Consistent with previous observations using murine bone marrow-derived dendritic cells, mutants lacking the capsule or MeOPN elicited enhanced transcription of IL-6 and IL-10 in mBMMs compared to wild-type C. jejuni. However, the lack of capsule and MeOPN did not alter IL-6 and IL-10 expression in chBMMs and hMDMs compared to C. jejuni WT. Phagocytosis assays showed the acapsular mutant was not impaired in uptake or net intracellular survival after phagocytosis in both chicken and human macrophages; however, the phagocytosis of the MeOPN mutant was significantly decreased in both chicken and human macrophages. In conclusion, differences in the response of macrophages of varying host origin to Campylobacter were detected. The absence of MeOPN modification on the capsule of C. jejuni did not alter the levels of innate cytokine expression in both chicken and human macrophages compared to the 11168H WT, but affected phagocytosis by host macrophages.

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KW - Bone Marrow

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KW - Campylobacter jejuni/genetics

KW - Chickens

KW - Cytokines/metabolism

KW - Humans

KW - Macrophages/metabolism

KW - Monocytes/metabolism

KW - Mutation

KW - Phosphoric Acids/metabolism

KW - Poultry Diseases/microbiology

KW - Rats

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DO - 10.1186/s13567-017-0501-y

M3 - Article

C2 - 29316981

VL - 49

JO - Veterinary Research

JF - Veterinary Research

SN - 0928-4249

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