Hydrogen and methane emissions from beef cattle and their rumen microbial community vary with diet, time after feeding and genotype

JA Rooke, RJ Wallace, C-A Duthie, N McKain, S Motta de Souza, JJ Hyslop, DW Ross, A Waterhouse, R Roehe

Research output: Contribution to journalArticle

45 Citations (Scopus)
6 Downloads (Pure)

Abstract

The aims of the present study were to quantify hydrogen (H2) and methane (CH4) emissions from beef cattle under different dietary conditions and to assess how cattle genotype and rumen microbial community affected these emissions. A total of thirty-six Aberdeen Angus-sired (AAx) and thirty-six Limousin-sired (LIMx) steers were fed two diets with forage:concentrate ratios (DM basis) of either 8:92 (concentrate) or 52:48 (mixed). Each diet was fed to eighteen animals of each genotype. Methane (CH4) and H2 emissions were measured individually in indirect respiration chambers. H2 emissions (mmol/min) varied greatly throughout the day, being highest after feed consumption, and averaged about 0·10 mol H2/mol CH4. Higher H2 emissions (mol/kg DM intake) were recorded in steers fed the mixed diet. Higher CH4 emissions (mol/d and mol/kg DM intake) were recorded in steers fed the mixed diet (P,0·001); the AAx steers produced more CH4 on a daily basis (mol/d, P,0·05) but not on a DM intake basis (mol/kg DM intake). Archaea (P¼0·002) and protozoa (P,0·001) were found to be more abundant and total bacteria (P,0·001) less abundant (P,0·001) on feeding the mixed diet. The relative abundance of Clostridium cluster IV was found to be greater (P,0·001) and that of cluster XIVa (P¼0·025) lower on feeding the mixed diet. The relative abundance of Bacteroides plus Prevotella was greater (P¼0·018) and that of Clostridium cluster IV lower (P¼0·031) in the LIMx steers. There were no significant relationships between H2 emissions and microbial abundance. In conclusion, the rate of H2 production immediately after feeding may lead to transient overloading of methanogenic archaea capacity to use H2, resulting in peaks in H2 emissions from beef cattle.
Original languageEnglish
Pages (from-to)398 - 407
Number of pages10
JournalBritish Journal of Nutrition
Volume112
Issue number3
DOIs
Publication statusPrint publication - 2014

Fingerprint

beef cattle
methane
hydrogen
microbial communities
rumen
genotype
diet
Clostridium
Archaea
concentrates
Prevotella
Limousin (cattle breed)
hydrogen production
Angus
Protozoa
feed intake
forage
cattle
bacteria
animals

Bibliographical note

1023322

Keywords

  • Beef cattle
  • Hydrogen
  • Methane
  • Rumen microbial community

Cite this

Rooke, JA ; Wallace, RJ ; Duthie, C-A ; McKain, N ; Motta de Souza, S ; Hyslop, JJ ; Ross, DW ; Waterhouse, A ; Roehe, R. / Hydrogen and methane emissions from beef cattle and their rumen microbial community vary with diet, time after feeding and genotype. In: British Journal of Nutrition. 2014 ; Vol. 112, No. 3. pp. 398 - 407.
@article{90295d87856c458692c7054b9e796c56,
title = "Hydrogen and methane emissions from beef cattle and their rumen microbial community vary with diet, time after feeding and genotype",
abstract = "The aims of the present study were to quantify hydrogen (H2) and methane (CH4) emissions from beef cattle under different dietary conditions and to assess how cattle genotype and rumen microbial community affected these emissions. A total of thirty-six Aberdeen Angus-sired (AAx) and thirty-six Limousin-sired (LIMx) steers were fed two diets with forage:concentrate ratios (DM basis) of either 8:92 (concentrate) or 52:48 (mixed). Each diet was fed to eighteen animals of each genotype. Methane (CH4) and H2 emissions were measured individually in indirect respiration chambers. H2 emissions (mmol/min) varied greatly throughout the day, being highest after feed consumption, and averaged about 0·10 mol H2/mol CH4. Higher H2 emissions (mol/kg DM intake) were recorded in steers fed the mixed diet. Higher CH4 emissions (mol/d and mol/kg DM intake) were recorded in steers fed the mixed diet (P,0·001); the AAx steers produced more CH4 on a daily basis (mol/d, P,0·05) but not on a DM intake basis (mol/kg DM intake). Archaea (P¼0·002) and protozoa (P,0·001) were found to be more abundant and total bacteria (P,0·001) less abundant (P,0·001) on feeding the mixed diet. The relative abundance of Clostridium cluster IV was found to be greater (P,0·001) and that of cluster XIVa (P¼0·025) lower on feeding the mixed diet. The relative abundance of Bacteroides plus Prevotella was greater (P¼0·018) and that of Clostridium cluster IV lower (P¼0·031) in the LIMx steers. There were no significant relationships between H2 emissions and microbial abundance. In conclusion, the rate of H2 production immediately after feeding may lead to transient overloading of methanogenic archaea capacity to use H2, resulting in peaks in H2 emissions from beef cattle.",
keywords = "Beef cattle, Hydrogen, Methane, Rumen microbial community",
author = "JA Rooke and RJ Wallace and C-A Duthie and N McKain and {Motta de Souza}, S and JJ Hyslop and DW Ross and A Waterhouse and R Roehe",
note = "1023322",
year = "2014",
doi = "10.1017/S0007114514000932",
language = "English",
volume = "112",
pages = "398 -- 407",
journal = "British Journal of Nutrition",
number = "3",

}

Hydrogen and methane emissions from beef cattle and their rumen microbial community vary with diet, time after feeding and genotype. / Rooke, JA; Wallace, RJ; Duthie, C-A; McKain, N; Motta de Souza, S; Hyslop, JJ; Ross, DW; Waterhouse, A; Roehe, R.

In: British Journal of Nutrition, Vol. 112, No. 3, 2014, p. 398 - 407.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Hydrogen and methane emissions from beef cattle and their rumen microbial community vary with diet, time after feeding and genotype

AU - Rooke, JA

AU - Wallace, RJ

AU - Duthie, C-A

AU - McKain, N

AU - Motta de Souza, S

AU - Hyslop, JJ

AU - Ross, DW

AU - Waterhouse, A

AU - Roehe, R

N1 - 1023322

PY - 2014

Y1 - 2014

N2 - The aims of the present study were to quantify hydrogen (H2) and methane (CH4) emissions from beef cattle under different dietary conditions and to assess how cattle genotype and rumen microbial community affected these emissions. A total of thirty-six Aberdeen Angus-sired (AAx) and thirty-six Limousin-sired (LIMx) steers were fed two diets with forage:concentrate ratios (DM basis) of either 8:92 (concentrate) or 52:48 (mixed). Each diet was fed to eighteen animals of each genotype. Methane (CH4) and H2 emissions were measured individually in indirect respiration chambers. H2 emissions (mmol/min) varied greatly throughout the day, being highest after feed consumption, and averaged about 0·10 mol H2/mol CH4. Higher H2 emissions (mol/kg DM intake) were recorded in steers fed the mixed diet. Higher CH4 emissions (mol/d and mol/kg DM intake) were recorded in steers fed the mixed diet (P,0·001); the AAx steers produced more CH4 on a daily basis (mol/d, P,0·05) but not on a DM intake basis (mol/kg DM intake). Archaea (P¼0·002) and protozoa (P,0·001) were found to be more abundant and total bacteria (P,0·001) less abundant (P,0·001) on feeding the mixed diet. The relative abundance of Clostridium cluster IV was found to be greater (P,0·001) and that of cluster XIVa (P¼0·025) lower on feeding the mixed diet. The relative abundance of Bacteroides plus Prevotella was greater (P¼0·018) and that of Clostridium cluster IV lower (P¼0·031) in the LIMx steers. There were no significant relationships between H2 emissions and microbial abundance. In conclusion, the rate of H2 production immediately after feeding may lead to transient overloading of methanogenic archaea capacity to use H2, resulting in peaks in H2 emissions from beef cattle.

AB - The aims of the present study were to quantify hydrogen (H2) and methane (CH4) emissions from beef cattle under different dietary conditions and to assess how cattle genotype and rumen microbial community affected these emissions. A total of thirty-six Aberdeen Angus-sired (AAx) and thirty-six Limousin-sired (LIMx) steers were fed two diets with forage:concentrate ratios (DM basis) of either 8:92 (concentrate) or 52:48 (mixed). Each diet was fed to eighteen animals of each genotype. Methane (CH4) and H2 emissions were measured individually in indirect respiration chambers. H2 emissions (mmol/min) varied greatly throughout the day, being highest after feed consumption, and averaged about 0·10 mol H2/mol CH4. Higher H2 emissions (mol/kg DM intake) were recorded in steers fed the mixed diet. Higher CH4 emissions (mol/d and mol/kg DM intake) were recorded in steers fed the mixed diet (P,0·001); the AAx steers produced more CH4 on a daily basis (mol/d, P,0·05) but not on a DM intake basis (mol/kg DM intake). Archaea (P¼0·002) and protozoa (P,0·001) were found to be more abundant and total bacteria (P,0·001) less abundant (P,0·001) on feeding the mixed diet. The relative abundance of Clostridium cluster IV was found to be greater (P,0·001) and that of cluster XIVa (P¼0·025) lower on feeding the mixed diet. The relative abundance of Bacteroides plus Prevotella was greater (P¼0·018) and that of Clostridium cluster IV lower (P¼0·031) in the LIMx steers. There were no significant relationships between H2 emissions and microbial abundance. In conclusion, the rate of H2 production immediately after feeding may lead to transient overloading of methanogenic archaea capacity to use H2, resulting in peaks in H2 emissions from beef cattle.

KW - Beef cattle

KW - Hydrogen

KW - Methane

KW - Rumen microbial community

U2 - 10.1017/S0007114514000932

DO - 10.1017/S0007114514000932

M3 - Article

VL - 112

SP - 398

EP - 407

JO - British Journal of Nutrition

JF - British Journal of Nutrition

IS - 3

ER -