Identification and characterization of miRNA transcriptome in potato by high-throughput sequencing

Runxuan Zhang, David Marshall, Glenn J. Bryan, Csaba Hornyik

Research output: Contribution to journalArticlepeer-review

79 Citations (Scopus)

Abstract

Micro RNAs (miRNAs) represent a class of short, non-coding, endogenous RNAs which play important roles in post-transcriptional regulation of gene expression. While the diverse functions of miRNAs in model plants have been well studied, the impact of miRNAs in crop plant biology is poorly understood. Here we used high-throughput sequencing and bioinformatics analysis to analyze miRNAs in the tuber bearing crop potato (Solanum tuberosum). Small RNAs were analysed from leaf and stolon tissues. 28 conserved miRNA families were found and potato-specific miRNAs were identified and validated by RNA gel blot hybridization. The size, origin and predicted targets of conserved and potato specific miRNAs are described. The large number of miRNAs and complex population of small RNAs in potato suggest important roles for these non-coding RNAs in diverse physiological and metabolic pathways.
Original languageEnglish
Article number e57233
JournalPLoS ONE
Volume8
Issue number2
DOIs
Publication statusPrint publication - 21 Feb 2013
Externally publishedYes

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