Identification of microRNA-135b in stool as a potential noninvasive biomarker for colorectal cancer and adenoma

Chung Wah Wu; Siew Chien Ng; Yujuan Dong; Linwei Tian; Simon Siu Man Ng; Wing Wa Leung; Wai Tak Law; Tung On Yau; Francis Ka Leung Chan; Joseph Jao Yiu Sung; Jun Yu

doi:10.1158/1078-0432.CCR-13-1750

Identification of microRNA-135b in stool as a potential noninvasive biomarker for colorectal cancer and adenoma

Chung Wah Wu, Siew Chien Ng, Yujuan Dong, Linwei Tian, Simon Siu Man Ng, Wing Wa Leung, Wai Tak Law, Tung On Yau, Francis Ka Leung Chan, Joseph Jao Yiu Sung, Jun Yu

Research output: Contribution to journal › Article › peer-review

122 Citations (Scopus)

Abstract

PURPOSE: Detecting microRNA (miRNA) in stool is a novel approach for colorectal cancer (CRC) screening. This study aimed to identify stool-based miRNA as noninvasive biomarkers for detection of CRC and adenoma.

EXPERIMENTAL DESIGN: A miRNA expression array covering 667 human miRNAs was performed on five pairs of CRC and two pairs of advanced adenoma tissues. The most upregulated miRNAs were validated in 40 pairs of CRC tissues, 16 pairs of advanced adenoma tissues, and 424 stool samples, including 104 CRCs, 169 adenomas, 42 inflammatory bowel diseases (IBD), and 109 healthy controls. miRNA levels were followed-up after removal of lesions.

RESULTS: In an array analysis, miR-31 and miR-135b were the most upregulated miRNAs in CRC and advanced adenoma as compared with their adjacent normal tissues (>13-fold increase). In stool samples, level of miR-135b was significantly higher in subjects with CRC (P < 0.0001) or adenomas (P < 0.0001), but not in patients with IBD compared with controls. miR-135b showed a significant increasing trend across the adenoma to cancer sequence (P < 0.0001). Levels of miR-31 were not significantly different among groups. The sensitivity of stool mR-135b was 78% for CRC, 73% for advanced adenoma, and 65% for any adenoma, respectively, with a specificity of 68%. No significant difference in the miR-135b level was found between proximal and distal colorectal lesions. Stool miR-135b dropped significantly upon removal of CRC or advanced adenoma (P < 0.0001).

Original language	English
Pages (from-to)	2994-3002
Number of pages	9
Journal	Clinical Cancer Research
Volume	20
Issue number	11
DOIs	https://doi.org/10.1158/1078-0432.CCR-13-1750
Publication status	Print publication - 1 Jun 2014
Externally published	Yes

Keywords

Adenocarcinoma/diagnosis
Adenoma/diagnosis
Aged
Biomarkers, Tumor/analysis
Case-Control Studies
Colorectal Neoplasms/diagnosis
Early Detection of Cancer/methods
Feces/chemistry
Female
Humans
Male
MicroRNAs/analysis
Middle Aged
Oligonucleotide Array Sequence Analysis
Real-Time Polymerase Chain Reaction
Sensitivity and Specificity

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1158/1078-0432.CCR-13-1750

Cite this

@article{00fd66b91ed04128b621350cc78492b2,

title = "Identification of microRNA-135b in stool as a potential noninvasive biomarker for colorectal cancer and adenoma",

abstract = "PURPOSE: Detecting microRNA (miRNA) in stool is a novel approach for colorectal cancer (CRC) screening. This study aimed to identify stool-based miRNA as noninvasive biomarkers for detection of CRC and adenoma.EXPERIMENTAL DESIGN: A miRNA expression array covering 667 human miRNAs was performed on five pairs of CRC and two pairs of advanced adenoma tissues. The most upregulated miRNAs were validated in 40 pairs of CRC tissues, 16 pairs of advanced adenoma tissues, and 424 stool samples, including 104 CRCs, 169 adenomas, 42 inflammatory bowel diseases (IBD), and 109 healthy controls. miRNA levels were followed-up after removal of lesions.RESULTS: In an array analysis, miR-31 and miR-135b were the most upregulated miRNAs in CRC and advanced adenoma as compared with their adjacent normal tissues (>13-fold increase). In stool samples, level of miR-135b was significantly higher in subjects with CRC (P < 0.0001) or adenomas (P < 0.0001), but not in patients with IBD compared with controls. miR-135b showed a significant increasing trend across the adenoma to cancer sequence (P < 0.0001). Levels of miR-31 were not significantly different among groups. The sensitivity of stool mR-135b was 78% for CRC, 73% for advanced adenoma, and 65% for any adenoma, respectively, with a specificity of 68%. No significant difference in the miR-135b level was found between proximal and distal colorectal lesions. Stool miR-135b dropped significantly upon removal of CRC or advanced adenoma (P < 0.0001).CONCLUSION: Stool-based miR-135b can be used as a noninvasive biomarker for the detection of CRC and advanced adenoma. Clin Cancer Res; 20(11); 2994-3002. {\textcopyright}2014 AACR.",

keywords = "Adenocarcinoma/diagnosis, Adenoma/diagnosis, Aged, Biomarkers, Tumor/analysis, Case-Control Studies, Colorectal Neoplasms/diagnosis, Early Detection of Cancer/methods, Feces/chemistry, Female, Humans, Male, MicroRNAs/analysis, Middle Aged, Oligonucleotide Array Sequence Analysis, Real-Time Polymerase Chain Reaction, Sensitivity and Specificity",

author = "Wu, {Chung Wah} and Ng, {Siew Chien} and Yujuan Dong and Linwei Tian and Ng, {Simon Siu Man} and Leung, {Wing Wa} and Law, {Wai Tak} and Yau, {Tung On} and Chan, {Francis Ka Leung} and Sung, {Joseph Jao Yiu} and Jun Yu",

year = "2014",

month = jun,

day = "1",

doi = "10.1158/1078-0432.CCR-13-1750",

language = "English",

volume = "20",

pages = "2994--3002",

journal = "Clinical Cancer Research",

issn = "1078-0432",

publisher = "American Association for Cancer Research Inc.",

number = "11",

}

TY - JOUR

T1 - Identification of microRNA-135b in stool as a potential noninvasive biomarker for colorectal cancer and adenoma

AU - Wu, Chung Wah

AU - Ng, Siew Chien

AU - Dong, Yujuan

AU - Tian, Linwei

AU - Ng, Simon Siu Man

AU - Leung, Wing Wa

AU - Law, Wai Tak

AU - Yau, Tung On

AU - Chan, Francis Ka Leung

AU - Sung, Joseph Jao Yiu

AU - Yu, Jun

PY - 2014/6/1

Y1 - 2014/6/1

N2 - PURPOSE: Detecting microRNA (miRNA) in stool is a novel approach for colorectal cancer (CRC) screening. This study aimed to identify stool-based miRNA as noninvasive biomarkers for detection of CRC and adenoma.EXPERIMENTAL DESIGN: A miRNA expression array covering 667 human miRNAs was performed on five pairs of CRC and two pairs of advanced adenoma tissues. The most upregulated miRNAs were validated in 40 pairs of CRC tissues, 16 pairs of advanced adenoma tissues, and 424 stool samples, including 104 CRCs, 169 adenomas, 42 inflammatory bowel diseases (IBD), and 109 healthy controls. miRNA levels were followed-up after removal of lesions.RESULTS: In an array analysis, miR-31 and miR-135b were the most upregulated miRNAs in CRC and advanced adenoma as compared with their adjacent normal tissues (>13-fold increase). In stool samples, level of miR-135b was significantly higher in subjects with CRC (P < 0.0001) or adenomas (P < 0.0001), but not in patients with IBD compared with controls. miR-135b showed a significant increasing trend across the adenoma to cancer sequence (P < 0.0001). Levels of miR-31 were not significantly different among groups. The sensitivity of stool mR-135b was 78% for CRC, 73% for advanced adenoma, and 65% for any adenoma, respectively, with a specificity of 68%. No significant difference in the miR-135b level was found between proximal and distal colorectal lesions. Stool miR-135b dropped significantly upon removal of CRC or advanced adenoma (P < 0.0001).CONCLUSION: Stool-based miR-135b can be used as a noninvasive biomarker for the detection of CRC and advanced adenoma. Clin Cancer Res; 20(11); 2994-3002. ©2014 AACR.

AB - PURPOSE: Detecting microRNA (miRNA) in stool is a novel approach for colorectal cancer (CRC) screening. This study aimed to identify stool-based miRNA as noninvasive biomarkers for detection of CRC and adenoma.EXPERIMENTAL DESIGN: A miRNA expression array covering 667 human miRNAs was performed on five pairs of CRC and two pairs of advanced adenoma tissues. The most upregulated miRNAs were validated in 40 pairs of CRC tissues, 16 pairs of advanced adenoma tissues, and 424 stool samples, including 104 CRCs, 169 adenomas, 42 inflammatory bowel diseases (IBD), and 109 healthy controls. miRNA levels were followed-up after removal of lesions.RESULTS: In an array analysis, miR-31 and miR-135b were the most upregulated miRNAs in CRC and advanced adenoma as compared with their adjacent normal tissues (>13-fold increase). In stool samples, level of miR-135b was significantly higher in subjects with CRC (P < 0.0001) or adenomas (P < 0.0001), but not in patients with IBD compared with controls. miR-135b showed a significant increasing trend across the adenoma to cancer sequence (P < 0.0001). Levels of miR-31 were not significantly different among groups. The sensitivity of stool mR-135b was 78% for CRC, 73% for advanced adenoma, and 65% for any adenoma, respectively, with a specificity of 68%. No significant difference in the miR-135b level was found between proximal and distal colorectal lesions. Stool miR-135b dropped significantly upon removal of CRC or advanced adenoma (P < 0.0001).CONCLUSION: Stool-based miR-135b can be used as a noninvasive biomarker for the detection of CRC and advanced adenoma. Clin Cancer Res; 20(11); 2994-3002. ©2014 AACR.

KW - Adenocarcinoma/diagnosis

KW - Adenoma/diagnosis

KW - Aged

KW - Biomarkers, Tumor/analysis

KW - Case-Control Studies

KW - Colorectal Neoplasms/diagnosis

KW - Early Detection of Cancer/methods

KW - Feces/chemistry

KW - Female

KW - Humans

KW - Male

KW - MicroRNAs/analysis

KW - Middle Aged

KW - Oligonucleotide Array Sequence Analysis

KW - Real-Time Polymerase Chain Reaction

KW - Sensitivity and Specificity

U2 - 10.1158/1078-0432.CCR-13-1750

DO - 10.1158/1078-0432.CCR-13-1750

M3 - Article

C2 - 24691020

SN - 1078-0432

VL - 20

SP - 2994

EP - 3002

JO - Clinical Cancer Research

JF - Clinical Cancer Research

IS - 11

ER -

Identification of microRNA-135b in stool as a potential noninvasive biomarker for colorectal cancer and adenoma

Abstract

Keywords

UN SDGs

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