Immune-associated traits measured in milk of Holstein-Friesian cows as proxies for blood serum measurements

SJ Denholm, TN McNeilly, G Banos, MP Coffey, GC Russell, A Bagnall, MC Mitchell, E Wall

Research output: Contribution to journalArticle

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Abstract

Previous work has highlighted that immune-associated (IA) traits measurable in blood are associated with health, productivity and reproduction in dairy cows. The aim of the present study was to determine relationships between immune associated traits measured in blood serum and those simultaneously measured in milk as well as their association with disease phenotypes. All animals were Holstein-Friesian cows from the Langhill research herd (n=546) housed at the SRUC Dairy Research Centre in Scotland. Milk and serum samples were collected on 20 separate occasions between July 2010 and March 2015 and analyzed by ELISA for haptoglobin (Hp), tumor necrosis factor alpha (TNF-α) and for natural antibodies binding keyhole limpet hemocyanin (NAbKLH) and lipopolysaccharide (NabLPS). Data were analyzed using mixed linear models that included pedigree information. Analyses revealed positive phenotypic correlations between milk and serum NAb (0.59 ≤ r ≤ 0.77), Hp (r=0.37) and TNF-α (r=0.12). Milk and serum NAb were also found to have a strong genetic correlation (0.81 ≤ r ≤ 0.94) and were both genetically correlated with cow lameness (0.66 and 0.79 for milk NAbKLH and serum NAbLPS respectively). Clinical mastitis was found to be phenotypically correlated with both milk and serum Hp (0.09 ≤ r ≤ 0.23). Serum Hp was also strongly genetically correlated with other cellular immune-associated traits such as %NKp46+ (0.35) and %PBMC (-0.90). Similarly, genetic correlations were found to exist between serum TNF-α and %NKp46+ (0.22), %PBMC (0.41) and %lymphocytes (0.47). Excluding serum Hp, all milk and serum IA traits were repeatable ranging from 0.11 (milk Hp) to 0.43 (serum NAbLPS). Between-animal variation was highest in milk and serum NAb (0.34 – 0.43) and significant estimates of heritability were also observed in milk and serum NAb (0.17 – 0.37). Our findings show that certain immune associated traits, such as NAbKLH and NAbLPS, found in milk and serum are strongly correlated and highlight the potential of using routinely collected milk samples as a less invasive and cost-effective source of informative data for predictive modeling of animal immune associated traits.
Original languageEnglish
Pages (from-to)10248 - 10258
Number of pages11
JournalJournal of Dairy Science
Volume101
Issue number11
Early online date29 Aug 2018
DOIs
Publication statusFirst published - 29 Aug 2018

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blood serum
Holstein
cows
milk
haptoglobins
tumor necrosis factor-alpha
mononuclear leukocytes
genetic correlation
antibodies
animals
phenotypic correlation
lameness
pedigree
Scotland
lipopolysaccharides
mastitis
antiserum
dairies
heritability
lymphocytes

Bibliographical note

1024416
1032201

Keywords

  • Correlation
  • Dairy cow
  • Immune-associated trait
  • Natural antibody

Cite this

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title = "Immune-associated traits measured in milk of Holstein-Friesian cows as proxies for blood serum measurements",
abstract = "Previous work has highlighted that immune-associated (IA) traits measurable in blood are associated with health, productivity and reproduction in dairy cows. The aim of the present study was to determine relationships between immune associated traits measured in blood serum and those simultaneously measured in milk as well as their association with disease phenotypes. All animals were Holstein-Friesian cows from the Langhill research herd (n=546) housed at the SRUC Dairy Research Centre in Scotland. Milk and serum samples were collected on 20 separate occasions between July 2010 and March 2015 and analyzed by ELISA for haptoglobin (Hp), tumor necrosis factor alpha (TNF-α) and for natural antibodies binding keyhole limpet hemocyanin (NAbKLH) and lipopolysaccharide (NabLPS). Data were analyzed using mixed linear models that included pedigree information. Analyses revealed positive phenotypic correlations between milk and serum NAb (0.59 ≤ r ≤ 0.77), Hp (r=0.37) and TNF-α (r=0.12). Milk and serum NAb were also found to have a strong genetic correlation (0.81 ≤ r ≤ 0.94) and were both genetically correlated with cow lameness (0.66 and 0.79 for milk NAbKLH and serum NAbLPS respectively). Clinical mastitis was found to be phenotypically correlated with both milk and serum Hp (0.09 ≤ r ≤ 0.23). Serum Hp was also strongly genetically correlated with other cellular immune-associated traits such as {\%}NKp46+ (0.35) and {\%}PBMC (-0.90). Similarly, genetic correlations were found to exist between serum TNF-α and {\%}NKp46+ (0.22), {\%}PBMC (0.41) and {\%}lymphocytes (0.47). Excluding serum Hp, all milk and serum IA traits were repeatable ranging from 0.11 (milk Hp) to 0.43 (serum NAbLPS). Between-animal variation was highest in milk and serum NAb (0.34 – 0.43) and significant estimates of heritability were also observed in milk and serum NAb (0.17 – 0.37). Our findings show that certain immune associated traits, such as NAbKLH and NAbLPS, found in milk and serum are strongly correlated and highlight the potential of using routinely collected milk samples as a less invasive and cost-effective source of informative data for predictive modeling of animal immune associated traits.",
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Immune-associated traits measured in milk of Holstein-Friesian cows as proxies for blood serum measurements. / Denholm, SJ; McNeilly, TN; Banos, G; Coffey, MP; Russell, GC; Bagnall, A; Mitchell, MC; Wall, E.

In: Journal of Dairy Science, Vol. 101, No. 11, 29.08.2018, p. 10248 - 10258.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Immune-associated traits measured in milk of Holstein-Friesian cows as proxies for blood serum measurements

AU - Denholm, SJ

AU - McNeilly, TN

AU - Banos, G

AU - Coffey, MP

AU - Russell, GC

AU - Bagnall, A

AU - Mitchell, MC

AU - Wall, E

N1 - 1024416 1032201

PY - 2018/8/29

Y1 - 2018/8/29

N2 - Previous work has highlighted that immune-associated (IA) traits measurable in blood are associated with health, productivity and reproduction in dairy cows. The aim of the present study was to determine relationships between immune associated traits measured in blood serum and those simultaneously measured in milk as well as their association with disease phenotypes. All animals were Holstein-Friesian cows from the Langhill research herd (n=546) housed at the SRUC Dairy Research Centre in Scotland. Milk and serum samples were collected on 20 separate occasions between July 2010 and March 2015 and analyzed by ELISA for haptoglobin (Hp), tumor necrosis factor alpha (TNF-α) and for natural antibodies binding keyhole limpet hemocyanin (NAbKLH) and lipopolysaccharide (NabLPS). Data were analyzed using mixed linear models that included pedigree information. Analyses revealed positive phenotypic correlations between milk and serum NAb (0.59 ≤ r ≤ 0.77), Hp (r=0.37) and TNF-α (r=0.12). Milk and serum NAb were also found to have a strong genetic correlation (0.81 ≤ r ≤ 0.94) and were both genetically correlated with cow lameness (0.66 and 0.79 for milk NAbKLH and serum NAbLPS respectively). Clinical mastitis was found to be phenotypically correlated with both milk and serum Hp (0.09 ≤ r ≤ 0.23). Serum Hp was also strongly genetically correlated with other cellular immune-associated traits such as %NKp46+ (0.35) and %PBMC (-0.90). Similarly, genetic correlations were found to exist between serum TNF-α and %NKp46+ (0.22), %PBMC (0.41) and %lymphocytes (0.47). Excluding serum Hp, all milk and serum IA traits were repeatable ranging from 0.11 (milk Hp) to 0.43 (serum NAbLPS). Between-animal variation was highest in milk and serum NAb (0.34 – 0.43) and significant estimates of heritability were also observed in milk and serum NAb (0.17 – 0.37). Our findings show that certain immune associated traits, such as NAbKLH and NAbLPS, found in milk and serum are strongly correlated and highlight the potential of using routinely collected milk samples as a less invasive and cost-effective source of informative data for predictive modeling of animal immune associated traits.

AB - Previous work has highlighted that immune-associated (IA) traits measurable in blood are associated with health, productivity and reproduction in dairy cows. The aim of the present study was to determine relationships between immune associated traits measured in blood serum and those simultaneously measured in milk as well as their association with disease phenotypes. All animals were Holstein-Friesian cows from the Langhill research herd (n=546) housed at the SRUC Dairy Research Centre in Scotland. Milk and serum samples were collected on 20 separate occasions between July 2010 and March 2015 and analyzed by ELISA for haptoglobin (Hp), tumor necrosis factor alpha (TNF-α) and for natural antibodies binding keyhole limpet hemocyanin (NAbKLH) and lipopolysaccharide (NabLPS). Data were analyzed using mixed linear models that included pedigree information. Analyses revealed positive phenotypic correlations between milk and serum NAb (0.59 ≤ r ≤ 0.77), Hp (r=0.37) and TNF-α (r=0.12). Milk and serum NAb were also found to have a strong genetic correlation (0.81 ≤ r ≤ 0.94) and were both genetically correlated with cow lameness (0.66 and 0.79 for milk NAbKLH and serum NAbLPS respectively). Clinical mastitis was found to be phenotypically correlated with both milk and serum Hp (0.09 ≤ r ≤ 0.23). Serum Hp was also strongly genetically correlated with other cellular immune-associated traits such as %NKp46+ (0.35) and %PBMC (-0.90). Similarly, genetic correlations were found to exist between serum TNF-α and %NKp46+ (0.22), %PBMC (0.41) and %lymphocytes (0.47). Excluding serum Hp, all milk and serum IA traits were repeatable ranging from 0.11 (milk Hp) to 0.43 (serum NAbLPS). Between-animal variation was highest in milk and serum NAb (0.34 – 0.43) and significant estimates of heritability were also observed in milk and serum NAb (0.17 – 0.37). Our findings show that certain immune associated traits, such as NAbKLH and NAbLPS, found in milk and serum are strongly correlated and highlight the potential of using routinely collected milk samples as a less invasive and cost-effective source of informative data for predictive modeling of animal immune associated traits.

KW - Correlation

KW - Dairy cow

KW - Immune-associated trait

KW - Natural antibody

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U2 - 10.3168/jds.2018-14825

DO - 10.3168/jds.2018-14825

M3 - Article

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VL - 101

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JO - Journal of Dairy Science

JF - Journal of Dairy Science

SN - 0022-0302

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ER -