Impact of contrasting fish oil concentrations in the diet on ovine embryo development in vivo and of corresponding diet-specific derivative sera during in vitro culture

TG McEvoy, AG Onal, BK Speake, JJ Robinson

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

In the first of two experiments, numbers of ovulations and day 5 embryo yields were unaffected by fish oil (0, 3 or 6% w/w) in ewe diets; oestradiol concentrations and numbers of large ovarian follicles were increased. Fish oil tended to suppress embryo development and limit blastocyst diameter and cell number but not protein synthesis. In the second experiment IVF-derived embryos were cultured in medium supplemented with foetal calf serum (FCS) or, as in vitro equivalents to Experiment 1, serum from ewes fed rations supplemented with 0 (C), 3 (3F) or 6 (6F)% fish oil. Cleavage rates were not affected by serum source. More Grade 1 to 1.5 blastocysts were produced when using 3F serum (32% of blastocysts) than with C (27%; ns) or 6F (16%; P<0.05) or FCS (10%; P<0.005). It is suggested that at less than 3% inclusion in the diet of ewes, there may be beneficial effects of fish oil fatty acids on embryo production in vivo and of the corresponding ewe serum during in vitro culture. Higher concentrations are counter-productive.
Original languageEnglish
Pages (from-to)31 - 48
Number of pages18
JournalJournal of Animal and Feed Sciences
Volume21
Publication statusFirst published - 2012

Fingerprint

blood serum
fish oils
in vitro culture
ewes
embryogenesis
chemical derivatives
blastocyst
sheep
embryo (animal)
fetal bovine serum
diet
feed rations
ovarian follicles
ovulation
estradiol
protein synthesis
fatty acids
cells

Bibliographical note

1023378

Keywords

  • Antioxidants
  • Embryos
  • Fish oil
  • Oocytes
  • Ovulation rate
  • Sheep

Cite this

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title = "Impact of contrasting fish oil concentrations in the diet on ovine embryo development in vivo and of corresponding diet-specific derivative sera during in vitro culture",
abstract = "In the first of two experiments, numbers of ovulations and day 5 embryo yields were unaffected by fish oil (0, 3 or 6{\%} w/w) in ewe diets; oestradiol concentrations and numbers of large ovarian follicles were increased. Fish oil tended to suppress embryo development and limit blastocyst diameter and cell number but not protein synthesis. In the second experiment IVF-derived embryos were cultured in medium supplemented with foetal calf serum (FCS) or, as in vitro equivalents to Experiment 1, serum from ewes fed rations supplemented with 0 (C), 3 (3F) or 6 (6F){\%} fish oil. Cleavage rates were not affected by serum source. More Grade 1 to 1.5 blastocysts were produced when using 3F serum (32{\%} of blastocysts) than with C (27{\%}; ns) or 6F (16{\%}; P<0.05) or FCS (10{\%}; P<0.005). It is suggested that at less than 3{\%} inclusion in the diet of ewes, there may be beneficial effects of fish oil fatty acids on embryo production in vivo and of the corresponding ewe serum during in vitro culture. Higher concentrations are counter-productive.",
keywords = "Antioxidants, Embryos, Fish oil, Oocytes, Ovulation rate, Sheep",
author = "TG McEvoy and AG Onal and BK Speake and JJ Robinson",
note = "1023378",
year = "2012",
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journal = "Journal of Animal and Feed Sciences",
issn = "1230-1388",
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TY - JOUR

T1 - Impact of contrasting fish oil concentrations in the diet on ovine embryo development in vivo and of corresponding diet-specific derivative sera during in vitro culture

AU - McEvoy, TG

AU - Onal, AG

AU - Speake, BK

AU - Robinson, JJ

N1 - 1023378

PY - 2012

Y1 - 2012

N2 - In the first of two experiments, numbers of ovulations and day 5 embryo yields were unaffected by fish oil (0, 3 or 6% w/w) in ewe diets; oestradiol concentrations and numbers of large ovarian follicles were increased. Fish oil tended to suppress embryo development and limit blastocyst diameter and cell number but not protein synthesis. In the second experiment IVF-derived embryos were cultured in medium supplemented with foetal calf serum (FCS) or, as in vitro equivalents to Experiment 1, serum from ewes fed rations supplemented with 0 (C), 3 (3F) or 6 (6F)% fish oil. Cleavage rates were not affected by serum source. More Grade 1 to 1.5 blastocysts were produced when using 3F serum (32% of blastocysts) than with C (27%; ns) or 6F (16%; P<0.05) or FCS (10%; P<0.005). It is suggested that at less than 3% inclusion in the diet of ewes, there may be beneficial effects of fish oil fatty acids on embryo production in vivo and of the corresponding ewe serum during in vitro culture. Higher concentrations are counter-productive.

AB - In the first of two experiments, numbers of ovulations and day 5 embryo yields were unaffected by fish oil (0, 3 or 6% w/w) in ewe diets; oestradiol concentrations and numbers of large ovarian follicles were increased. Fish oil tended to suppress embryo development and limit blastocyst diameter and cell number but not protein synthesis. In the second experiment IVF-derived embryos were cultured in medium supplemented with foetal calf serum (FCS) or, as in vitro equivalents to Experiment 1, serum from ewes fed rations supplemented with 0 (C), 3 (3F) or 6 (6F)% fish oil. Cleavage rates were not affected by serum source. More Grade 1 to 1.5 blastocysts were produced when using 3F serum (32% of blastocysts) than with C (27%; ns) or 6F (16%; P<0.05) or FCS (10%; P<0.005). It is suggested that at less than 3% inclusion in the diet of ewes, there may be beneficial effects of fish oil fatty acids on embryo production in vivo and of the corresponding ewe serum during in vitro culture. Higher concentrations are counter-productive.

KW - Antioxidants

KW - Embryos

KW - Fish oil

KW - Oocytes

KW - Ovulation rate

KW - Sheep

M3 - Article

VL - 21

SP - 31

EP - 48

JO - Journal of Animal and Feed Sciences

JF - Journal of Animal and Feed Sciences

SN - 1230-1388

ER -