In the Murine and Bovine Maternal Mammary Gland Signal Transducer and Activator of Transcription 3 is Activated in Clusters of Epithelial Cells around the Day of Birth

Laura J A Hardwick; Benjamin P Davies; Sara Pensa; Maedee Burge-Rogers; Claire Davies; André Figueiredo Baptista; Robert Knott; Ian S McCrone; Eleonora Po; Benjamin W Strugnell; Katie Waine; Paul Wood; Walid T Khaled; Huw D Summers; Paul Rees; John W Wills; Katherine Hughes

doi:10.1007/s10911-024-09561-5

In the Murine and Bovine Maternal Mammary Gland Signal Transducer and Activator of Transcription 3 is Activated in Clusters of Epithelial Cells around the Day of Birth

Laura J A Hardwick, Benjamin P Davies, Sara Pensa, Maedee Burge-Rogers, Claire Davies, André Figueiredo Baptista, Robert Knott, Ian S McCrone, Eleonora Po, Benjamin W Strugnell, Katie Waine, Paul Wood, Walid T Khaled, Huw D Summers, Paul Rees, John W Wills, Katherine Hughes^*

^*Corresponding author for this work

Research output: Contribution to journal › Report/ Case Report › peer-review

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Abstract

Signal transducers and activators of transcription (STAT) proteins regulate mammary development. Here we investigate the expression of phosphorylated STAT3 (pSTAT3) in the mouse and cow around the day of birth. We present localised colocation analysis, applicable to other mammary studies requiring identification of spatially congregated events. We demonstrate that pSTAT3-positive events are multifocally clustered in a non-random and statistically significant fashion. Arginase-1 expressing cells, consistent with macrophages, exhibit distinct clustering within the periparturient mammary gland. These findings represent a new facet of mammary STAT3 biology, and point to the presence of mammary sub-microenvironments.

Original language	English
Article number	10
Journal	Journal of Mammary Gland Biology and Neoplasia
Volume	29
Issue number	1
Early online date	9 May 2024
DOIs	https://doi.org/10.1007/s10911-024-09561-5
Publication status	First published - 9 May 2024

Bibliographical note

Keywords

Animals
Female
Cattle
Mammary Glands, Animal/metabolism
Mice
Epithelial Cells/metabolism
STAT3 Transcription Factor/metabolism
Phosphorylation
Pregnancy
Parturition/physiology
Signal Transduction
Parturition
STAT3
Mammary
Spatial statistics
Udder
Macrophage

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Hardwick, L. J. A., Davies, B. P., Pensa, S., Burge-Rogers, M., Davies, C., Baptista, A. F., Knott, R., S McCrone, I., Po, E., Strugnell, B. W., Waine, K., Wood, P., Khaled, W. T., Summers, H. D., Rees, P., Wills, J. W., & Hughes, K. (2024). In the Murine and Bovine Maternal Mammary Gland Signal Transducer and Activator of Transcription 3 is Activated in Clusters of Epithelial Cells around the Day of Birth. Journal of Mammary Gland Biology and Neoplasia, 29(1), Article 10. Advance online publication. https://doi.org/10.1007/s10911-024-09561-5

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title = "In the Murine and Bovine Maternal Mammary Gland Signal Transducer and Activator of Transcription 3 is Activated in Clusters of Epithelial Cells around the Day of Birth",

abstract = "Signal transducers and activators of transcription (STAT) proteins regulate mammary development. Here we investigate the expression of phosphorylated STAT3 (pSTAT3) in the mouse and cow around the day of birth. We present localised colocation analysis, applicable to other mammary studies requiring identification of spatially congregated events. We demonstrate that pSTAT3-positive events are multifocally clustered in a non-random and statistically significant fashion. Arginase-1 expressing cells, consistent with macrophages, exhibit distinct clustering within the periparturient mammary gland. These findings represent a new facet of mammary STAT3 biology, and point to the presence of mammary sub-microenvironments.",

keywords = "Animals, Female, Cattle, Mammary Glands, Animal/metabolism, Mice, Epithelial Cells/metabolism, STAT3 Transcription Factor/metabolism, Phosphorylation, Pregnancy, Parturition/physiology, Signal Transduction, Parturition, STAT3, Mammary, Spatial statistics, Udder, Macrophage",

author = "Hardwick, {Laura J A} and Davies, {Benjamin P} and Sara Pensa and Maedee Burge-Rogers and Claire Davies and Baptista, {Andr{\'e} Figueiredo} and Robert Knott and {S McCrone}, Ian and Eleonora Po and Strugnell, {Benjamin W} and Katie Waine and Paul Wood and Khaled, {Walid T} and Summers, {Huw D} and Paul Rees and Wills, {John W} and Katherine Hughes",

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year = "2024",

month = may,

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doi = "10.1007/s10911-024-09561-5",

language = "English",

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Hardwick, LJA, Davies, BP, Pensa, S, Burge-Rogers, M, Davies, C, Baptista, AF, Knott, R, S McCrone, I, Po, E, Strugnell, BW, Waine, K, Wood, P, Khaled, WT, Summers, HD, Rees, P, Wills, JW & Hughes, K 2024, 'In the Murine and Bovine Maternal Mammary Gland Signal Transducer and Activator of Transcription 3 is Activated in Clusters of Epithelial Cells around the Day of Birth', Journal of Mammary Gland Biology and Neoplasia, vol. 29, no. 1, 10. https://doi.org/10.1007/s10911-024-09561-5

In the Murine and Bovine Maternal Mammary Gland Signal Transducer and Activator of Transcription 3 is Activated in Clusters of Epithelial Cells around the Day of Birth. / Hardwick, Laura J A; Davies, Benjamin P; Pensa, Sara et al.
In: Journal of Mammary Gland Biology and Neoplasia, Vol. 29, No. 1, 10, 09.05.2024.

Research output: Contribution to journal › Report/ Case Report › peer-review

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T1 - In the Murine and Bovine Maternal Mammary Gland Signal Transducer and Activator of Transcription 3 is Activated in Clusters of Epithelial Cells around the Day of Birth

AU - Hardwick, Laura J A

AU - Davies, Benjamin P

AU - Pensa, Sara

AU - Burge-Rogers, Maedee

AU - Davies, Claire

AU - Baptista, André Figueiredo

AU - Knott, Robert

AU - S McCrone, Ian

AU - Po, Eleonora

AU - Strugnell, Benjamin W

AU - Waine, Katie

AU - Wood, Paul

AU - Khaled, Walid T

AU - Summers, Huw D

AU - Rees, Paul

AU - Wills, John W

AU - Hughes, Katherine

PY - 2024/5/9

Y1 - 2024/5/9

N2 - Signal transducers and activators of transcription (STAT) proteins regulate mammary development. Here we investigate the expression of phosphorylated STAT3 (pSTAT3) in the mouse and cow around the day of birth. We present localised colocation analysis, applicable to other mammary studies requiring identification of spatially congregated events. We demonstrate that pSTAT3-positive events are multifocally clustered in a non-random and statistically significant fashion. Arginase-1 expressing cells, consistent with macrophages, exhibit distinct clustering within the periparturient mammary gland. These findings represent a new facet of mammary STAT3 biology, and point to the presence of mammary sub-microenvironments.

AB - Signal transducers and activators of transcription (STAT) proteins regulate mammary development. Here we investigate the expression of phosphorylated STAT3 (pSTAT3) in the mouse and cow around the day of birth. We present localised colocation analysis, applicable to other mammary studies requiring identification of spatially congregated events. We demonstrate that pSTAT3-positive events are multifocally clustered in a non-random and statistically significant fashion. Arginase-1 expressing cells, consistent with macrophages, exhibit distinct clustering within the periparturient mammary gland. These findings represent a new facet of mammary STAT3 biology, and point to the presence of mammary sub-microenvironments.

KW - Animals

KW - Female

KW - Cattle

KW - Mammary Glands, Animal/metabolism

KW - Mice

KW - Epithelial Cells/metabolism

KW - STAT3 Transcription Factor/metabolism

KW - Phosphorylation

KW - Pregnancy

KW - Parturition/physiology

KW - Signal Transduction

KW - Parturition

KW - STAT3

KW - Mammary

KW - Spatial statistics

KW - Udder

KW - Macrophage

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U2 - 10.1007/s10911-024-09561-5

DO - 10.1007/s10911-024-09561-5

M3 - Report/ Case Report

C2 - 38722417

SN - 1083-3021

VL - 29

JO - Journal of Mammary Gland Biology and Neoplasia

JF - Journal of Mammary Gland Biology and Neoplasia

IS - 1

M1 - 10

ER -

In the Murine and Bovine Maternal Mammary Gland Signal Transducer and Activator of Transcription 3 is Activated in Clusters of Epithelial Cells around the Day of Birth

Abstract

Bibliographical note

Keywords

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