In the Murine and Bovine Maternal Mammary Gland Signal Transducer and Activator of Transcription 3 is Activated in Clusters of Epithelial Cells around the Day of Birth

Laura J A Hardwick, Benjamin P Davies, Sara Pensa, Maedee Burge-Rogers, Claire Davies, André Figueiredo Baptista, Robert Knott, Ian S McCrone, Eleonora Po, Benjamin W Strugnell, Katie Waine, Paul Wood, Walid T Khaled, Huw D Summers, Paul Rees, John W Wills, Katherine Hughes*

*Corresponding author for this work

    Research output: Contribution to journalReport/ Case Reportpeer-review

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    Abstract

    Signal transducers and activators of transcription (STAT) proteins regulate mammary development. Here we investigate the expression of phosphorylated STAT3 (pSTAT3) in the mouse and cow around the day of birth. We present localised colocation analysis, applicable to other mammary studies requiring identification of spatially congregated events. We demonstrate that pSTAT3-positive events are multifocally clustered in a non-random and statistically significant fashion. Arginase-1 expressing cells, consistent with macrophages, exhibit distinct clustering within the periparturient mammary gland. These findings represent a new facet of mammary STAT3 biology, and point to the presence of mammary sub-microenvironments.

    Original languageEnglish
    Article number10
    JournalJournal of Mammary Gland Biology and Neoplasia
    Volume29
    Issue number1
    Early online date9 May 2024
    DOIs
    Publication statusFirst published - 9 May 2024

    Bibliographical note

    © 2024. The Author(s).

    Keywords

    • Animals
    • Female
    • Cattle
    • Mammary Glands, Animal/metabolism
    • Mice
    • Epithelial Cells/metabolism
    • STAT3 Transcription Factor/metabolism
    • Phosphorylation
    • Pregnancy
    • Parturition/physiology
    • Signal Transduction

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