In vitro effect of clarithromycin and alginate lyase against helicobacter pylori biofilm

Francesca Bugli; Valentina Palmieri; Riccardo Torelli; Massimiliano Papi; Marco De Spirito; Margherita Cacaci; SG Galgano; Luca Masucci; Francesco Paroni Sterbini; Antonella Vella; Rosalia Graffeo; Brunella Posteraro; Maurizio Sanguinetti

doi:10.1002/btpr.2339

In vitro effect of clarithromycin and alginate lyase against helicobacter pylori biofilm

Francesca Bugli, Valentina Palmieri, Riccardo Torelli, Massimiliano Papi^*, Marco De Spirito, Margherita Cacaci, SG Galgano, Luca Masucci, Francesco Paroni Sterbini, Antonella Vella, Rosalia Graffeo, Brunella Posteraro, Maurizio Sanguinetti

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

26 Citations (Scopus)

Abstract

It is now established that the gastric pathogen Helicobacter pylori has the ability to form biofilms in vitro as well as on the human gastric mucosa. The aim of this study is to evaluate the antimicrobial effects of Clarithromycin on H. pylori biofilm and to enhance the effects of this antibiotic by combining it with Alginate Lyase, an enzyme degrading the polysaccharides present in the extracellular polymeric matrix forming the biofilm. We evaluated the Clarithromycin minimum inhibition concentration (MIC) on in vitro preformed biofilm of a H. pylori. Then the synergic effect of Clarithromycin and Alginate Lyase treatment has been quantified by using the Fractional Inhibitory Concentration index, measured by checkerboard microdilution assay. To clarify the mechanisms behind the effectiveness of this antibiofilm therapeutic combination, we used Atomic Force Microscopy to analyze modifications of bacterial morphology, percentage of bacillary or coccoid shaped bacteria cells and to quantify biofilm properties.

Original language	English
Pages (from-to)	1584-1591
Journal	Biotechnology Progress
Volume	32
Issue number	6
Early online date	18 Aug 2016
DOIs	https://doi.org/10.1002/btpr.2339
Publication status	Print publication - Nov 2016
Externally published	Yes

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title = "In vitro effect of clarithromycin and alginate lyase against helicobacter pylori biofilm",

abstract = "It is now established that the gastric pathogen Helicobacter pylori has the ability to form biofilms in vitro as well as on the human gastric mucosa. The aim of this study is to evaluate the antimicrobial effects of Clarithromycin on H. pylori biofilm and to enhance the effects of this antibiotic by combining it with Alginate Lyase, an enzyme degrading the polysaccharides present in the extracellular polymeric matrix forming the biofilm. We evaluated the Clarithromycin minimum inhibition concentration (MIC) on in vitro preformed biofilm of a H. pylori. Then the synergic effect of Clarithromycin and Alginate Lyase treatment has been quantified by using the Fractional Inhibitory Concentration index, measured by checkerboard microdilution assay. To clarify the mechanisms behind the effectiveness of this antibiofilm therapeutic combination, we used Atomic Force Microscopy to analyze modifications of bacterial morphology, percentage of bacillary or coccoid shaped bacteria cells and to quantify biofilm properties.",

author = "Francesca Bugli and Valentina Palmieri and Riccardo Torelli and Massimiliano Papi and {De Spirito}, Marco and Margherita Cacaci and SG Galgano and Luca Masucci and {Paroni Sterbini}, Francesco and Antonella Vella and Rosalia Graffeo and Brunella Posteraro and Maurizio Sanguinetti",

year = "2016",

month = nov,

doi = "10.1002/btpr.2339",

language = "English",

volume = "32",

pages = "1584--1591",

journal = "Biotechnology Progress",

issn = "8756-7938",

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T1 - In vitro effect of clarithromycin and alginate lyase against helicobacter pylori biofilm

AU - Bugli, Francesca

AU - Palmieri, Valentina

AU - Torelli, Riccardo

AU - Papi, Massimiliano

AU - De Spirito, Marco

AU - Cacaci, Margherita

AU - Galgano, SG

AU - Masucci, Luca

AU - Paroni Sterbini, Francesco

AU - Vella, Antonella

AU - Graffeo, Rosalia

AU - Posteraro, Brunella

AU - Sanguinetti, Maurizio

PY - 2016/11

Y1 - 2016/11

N2 - It is now established that the gastric pathogen Helicobacter pylori has the ability to form biofilms in vitro as well as on the human gastric mucosa. The aim of this study is to evaluate the antimicrobial effects of Clarithromycin on H. pylori biofilm and to enhance the effects of this antibiotic by combining it with Alginate Lyase, an enzyme degrading the polysaccharides present in the extracellular polymeric matrix forming the biofilm. We evaluated the Clarithromycin minimum inhibition concentration (MIC) on in vitro preformed biofilm of a H. pylori. Then the synergic effect of Clarithromycin and Alginate Lyase treatment has been quantified by using the Fractional Inhibitory Concentration index, measured by checkerboard microdilution assay. To clarify the mechanisms behind the effectiveness of this antibiofilm therapeutic combination, we used Atomic Force Microscopy to analyze modifications of bacterial morphology, percentage of bacillary or coccoid shaped bacteria cells and to quantify biofilm properties.

AB - It is now established that the gastric pathogen Helicobacter pylori has the ability to form biofilms in vitro as well as on the human gastric mucosa. The aim of this study is to evaluate the antimicrobial effects of Clarithromycin on H. pylori biofilm and to enhance the effects of this antibiotic by combining it with Alginate Lyase, an enzyme degrading the polysaccharides present in the extracellular polymeric matrix forming the biofilm. We evaluated the Clarithromycin minimum inhibition concentration (MIC) on in vitro preformed biofilm of a H. pylori. Then the synergic effect of Clarithromycin and Alginate Lyase treatment has been quantified by using the Fractional Inhibitory Concentration index, measured by checkerboard microdilution assay. To clarify the mechanisms behind the effectiveness of this antibiofilm therapeutic combination, we used Atomic Force Microscopy to analyze modifications of bacterial morphology, percentage of bacillary or coccoid shaped bacteria cells and to quantify biofilm properties.

U2 - 10.1002/btpr.2339

DO - 10.1002/btpr.2339

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SN - 8756-7938

VL - 32

SP - 1584

EP - 1591

JO - Biotechnology Progress

JF - Biotechnology Progress

IS - 6

ER -

In vitro effect of clarithromycin and alginate lyase against helicobacter pylori biofilm

Abstract

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