Infection strategy of Ramularia collo-cygni and development of Ramularia leaf spot on barley and alternative graminaceous hosts

M Kaczmarek, MJ Piotrowska, JM Fountaine, K Gorniak, GRD McGrann, A Armstrong, KM Wright, AC Newton, ND Havis

Research output: Contribution to journalArticle

12 Citations (Scopus)
1 Downloads (Pure)

Abstract

Ramularia leaf spot (RLS) is a newly important disease of barley across temperate regions worldwide. Despite this recent change in importance, the infection biology of the causal agent Ramularia collo-cygni (Rcc) remains poorly understood. Confocal microscopy of the infection process of two transgenic Rcc isolates, expressing either GFP or DsRed reporter markers, was combined with light microscopy during field infection to track the progression of Rcc in planta. Infection of stomata, including the development of a previously unreported stomatopodium structure, results in symptomless development and intercellular colonization of the mesophyll tissue. Transition to necrotrophy is associated with breakdown of host chloroplasts and the formation of aggregates of conidiophores. In addition to barley, Rcc forms a compatible interaction with winter wheat and a number of perennial grass species. An incompatible reaction was observed with two dicotyledonous species. These results provide further insights into the host interactions of this fungus and suggest that RLS could be a potential threat to other agriculturally important crops.
Original languageEnglish
Pages (from-to)45 - 55
Number of pages11
JournalPlant Pathology
Volume66
Issue number1
Early online date6 Jun 2016
DOIs
Publication statusFirst published - 6 Jun 2016

Fingerprint

leaf spot
Ramularia
Cygnus
alternative hosts
barley
infection
conidiophores
cell aggregates
Plantae
stomata
mesophyll
winter wheat
light microscopy
chloroplasts
genetically modified organisms
grasses
Biological Sciences
fungi
crops

Bibliographical note

1023370

Keywords

  • Host
  • Infection
  • Interaction
  • Ramularia collo-cygni

Cite this

Kaczmarek, M ; Piotrowska, MJ ; Fountaine, JM ; Gorniak, K ; McGrann, GRD ; Armstrong, A ; Wright, KM ; Newton, AC ; Havis, ND. / Infection strategy of Ramularia collo-cygni and development of Ramularia leaf spot on barley and alternative graminaceous hosts. In: Plant Pathology. 2016 ; Vol. 66, No. 1. pp. 45 - 55.
@article{301b09cd420e4f26b79735733982d393,
title = "Infection strategy of Ramularia collo-cygni and development of Ramularia leaf spot on barley and alternative graminaceous hosts",
abstract = "Ramularia leaf spot (RLS) is a newly important disease of barley across temperate regions worldwide. Despite this recent change in importance, the infection biology of the causal agent Ramularia collo-cygni (Rcc) remains poorly understood. Confocal microscopy of the infection process of two transgenic Rcc isolates, expressing either GFP or DsRed reporter markers, was combined with light microscopy during field infection to track the progression of Rcc in planta. Infection of stomata, including the development of a previously unreported stomatopodium structure, results in symptomless development and intercellular colonization of the mesophyll tissue. Transition to necrotrophy is associated with breakdown of host chloroplasts and the formation of aggregates of conidiophores. In addition to barley, Rcc forms a compatible interaction with winter wheat and a number of perennial grass species. An incompatible reaction was observed with two dicotyledonous species. These results provide further insights into the host interactions of this fungus and suggest that RLS could be a potential threat to other agriculturally important crops.",
keywords = "Host, Infection, Interaction, Ramularia collo-cygni",
author = "M Kaczmarek and MJ Piotrowska and JM Fountaine and K Gorniak and GRD McGrann and A Armstrong and KM Wright and AC Newton and ND Havis",
note = "1023370",
year = "2016",
month = "6",
day = "6",
doi = "10.1111/ppa.12552",
language = "English",
volume = "66",
pages = "45 -- 55",
journal = "Plant Pathology",
issn = "0032-0862",
publisher = "Wiley",
number = "1",

}

Kaczmarek, M, Piotrowska, MJ, Fountaine, JM, Gorniak, K, McGrann, GRD, Armstrong, A, Wright, KM, Newton, AC & Havis, ND 2016, 'Infection strategy of Ramularia collo-cygni and development of Ramularia leaf spot on barley and alternative graminaceous hosts', Plant Pathology, vol. 66, no. 1, pp. 45 - 55. https://doi.org/10.1111/ppa.12552

Infection strategy of Ramularia collo-cygni and development of Ramularia leaf spot on barley and alternative graminaceous hosts. / Kaczmarek, M; Piotrowska, MJ; Fountaine, JM; Gorniak, K; McGrann, GRD; Armstrong, A; Wright, KM; Newton, AC; Havis, ND.

In: Plant Pathology, Vol. 66, No. 1, 06.06.2016, p. 45 - 55.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Infection strategy of Ramularia collo-cygni and development of Ramularia leaf spot on barley and alternative graminaceous hosts

AU - Kaczmarek, M

AU - Piotrowska, MJ

AU - Fountaine, JM

AU - Gorniak, K

AU - McGrann, GRD

AU - Armstrong, A

AU - Wright, KM

AU - Newton, AC

AU - Havis, ND

N1 - 1023370

PY - 2016/6/6

Y1 - 2016/6/6

N2 - Ramularia leaf spot (RLS) is a newly important disease of barley across temperate regions worldwide. Despite this recent change in importance, the infection biology of the causal agent Ramularia collo-cygni (Rcc) remains poorly understood. Confocal microscopy of the infection process of two transgenic Rcc isolates, expressing either GFP or DsRed reporter markers, was combined with light microscopy during field infection to track the progression of Rcc in planta. Infection of stomata, including the development of a previously unreported stomatopodium structure, results in symptomless development and intercellular colonization of the mesophyll tissue. Transition to necrotrophy is associated with breakdown of host chloroplasts and the formation of aggregates of conidiophores. In addition to barley, Rcc forms a compatible interaction with winter wheat and a number of perennial grass species. An incompatible reaction was observed with two dicotyledonous species. These results provide further insights into the host interactions of this fungus and suggest that RLS could be a potential threat to other agriculturally important crops.

AB - Ramularia leaf spot (RLS) is a newly important disease of barley across temperate regions worldwide. Despite this recent change in importance, the infection biology of the causal agent Ramularia collo-cygni (Rcc) remains poorly understood. Confocal microscopy of the infection process of two transgenic Rcc isolates, expressing either GFP or DsRed reporter markers, was combined with light microscopy during field infection to track the progression of Rcc in planta. Infection of stomata, including the development of a previously unreported stomatopodium structure, results in symptomless development and intercellular colonization of the mesophyll tissue. Transition to necrotrophy is associated with breakdown of host chloroplasts and the formation of aggregates of conidiophores. In addition to barley, Rcc forms a compatible interaction with winter wheat and a number of perennial grass species. An incompatible reaction was observed with two dicotyledonous species. These results provide further insights into the host interactions of this fungus and suggest that RLS could be a potential threat to other agriculturally important crops.

KW - Host

KW - Infection

KW - Interaction

KW - Ramularia collo-cygni

U2 - 10.1111/ppa.12552

DO - 10.1111/ppa.12552

M3 - Article

VL - 66

SP - 45

EP - 55

JO - Plant Pathology

JF - Plant Pathology

SN - 0032-0862

IS - 1

ER -