Influence of 1-[(E)-2-(2-methyl-4-nitrophenyl)diaz-1-enyl]pyrrolidine-2-carboxylic acid and diphenyliodonium chloride on ruminal protein metabolism and ruminal microorganisms

F Floret, L C Chaudhary, W C Ellis, S El Hassan, N McKain, C J Newbold, R J Wallace

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The effects of 1-[(E)-2-(2-methyl-4-nitrophenyl)diaz-1-enyl]pyrrolidine-2-carboxy lic acid (LY29) and diphenyliodonium chloride (DIC) on the degradation of protein to ammonia were determined in a mixed rumen microbial population taken from sheep on a grass hay-concentrate diet. Both compounds decreased NH3 production by inhibiting deamination of amino acids. LY29, but not DIC, inhibited growth of the high-activity ammonia-producing species, Clostridium aminophilum and Clostridium sticklandii.

Original languageEnglish
Pages (from-to)3258-60
Number of pages3
JournalApplied and Environmental Microbiology
Volume65
Issue number7
Publication statusPrint publication - Jul 1999
Externally publishedYes

Fingerprint

pyrrolidines
rumen microorganisms
carboxylic acid
carboxylic acids
protein metabolism
Ammonia
Clostridium sticklandii
chlorides
ammonia
microorganism
metabolism
chloride
Clostridium aminophilum
Deamination
protein
Clostridium
Rumen
hay
Poaceae
sheep

Keywords

  • Animal Feed
  • Animals
  • Azetidines/pharmacology
  • Bacteria/drug effects
  • Biphenyl Compounds/pharmacology
  • Clostridium/drug effects
  • Deamination
  • Endopeptidases/metabolism
  • Enzyme Inhibitors
  • Onium Compounds/pharmacology
  • Poaceae
  • Proline/analogs & derivatives
  • Proteins/metabolism
  • Pyrrolidines/pharmacology
  • Rumen/metabolism
  • Sheep

Cite this

@article{992510fb2ec74b1db87e6be3ce300fef,
title = "Influence of 1-[(E)-2-(2-methyl-4-nitrophenyl)diaz-1-enyl]pyrrolidine-2-carboxylic acid and diphenyliodonium chloride on ruminal protein metabolism and ruminal microorganisms",
abstract = "The effects of 1-[(E)-2-(2-methyl-4-nitrophenyl)diaz-1-enyl]pyrrolidine-2-carboxy lic acid (LY29) and diphenyliodonium chloride (DIC) on the degradation of protein to ammonia were determined in a mixed rumen microbial population taken from sheep on a grass hay-concentrate diet. Both compounds decreased NH3 production by inhibiting deamination of amino acids. LY29, but not DIC, inhibited growth of the high-activity ammonia-producing species, Clostridium aminophilum and Clostridium sticklandii.",
keywords = "Animal Feed, Animals, Azetidines/pharmacology, Bacteria/drug effects, Biphenyl Compounds/pharmacology, Clostridium/drug effects, Deamination, Endopeptidases/metabolism, Enzyme Inhibitors, Onium Compounds/pharmacology, Poaceae, Proline/analogs & derivatives, Proteins/metabolism, Pyrrolidines/pharmacology, Rumen/metabolism, Sheep",
author = "F Floret and Chaudhary, {L C} and Ellis, {W C} and {El Hassan}, S and N McKain and Newbold, {C J} and Wallace, {R J}",
year = "1999",
month = "7",
language = "English",
volume = "65",
pages = "3258--60",
journal = "Applied and Environmental Microbiology",
issn = "0099-2240",
publisher = "American Society for Microbiology",
number = "7",

}

Influence of 1-[(E)-2-(2-methyl-4-nitrophenyl)diaz-1-enyl]pyrrolidine-2-carboxylic acid and diphenyliodonium chloride on ruminal protein metabolism and ruminal microorganisms. / Floret, F; Chaudhary, L C; Ellis, W C; El Hassan, S; McKain, N; Newbold, C J; Wallace, R J.

In: Applied and Environmental Microbiology, Vol. 65, No. 7, 07.1999, p. 3258-60.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Influence of 1-[(E)-2-(2-methyl-4-nitrophenyl)diaz-1-enyl]pyrrolidine-2-carboxylic acid and diphenyliodonium chloride on ruminal protein metabolism and ruminal microorganisms

AU - Floret, F

AU - Chaudhary, L C

AU - Ellis, W C

AU - El Hassan, S

AU - McKain, N

AU - Newbold, C J

AU - Wallace, R J

PY - 1999/7

Y1 - 1999/7

N2 - The effects of 1-[(E)-2-(2-methyl-4-nitrophenyl)diaz-1-enyl]pyrrolidine-2-carboxy lic acid (LY29) and diphenyliodonium chloride (DIC) on the degradation of protein to ammonia were determined in a mixed rumen microbial population taken from sheep on a grass hay-concentrate diet. Both compounds decreased NH3 production by inhibiting deamination of amino acids. LY29, but not DIC, inhibited growth of the high-activity ammonia-producing species, Clostridium aminophilum and Clostridium sticklandii.

AB - The effects of 1-[(E)-2-(2-methyl-4-nitrophenyl)diaz-1-enyl]pyrrolidine-2-carboxy lic acid (LY29) and diphenyliodonium chloride (DIC) on the degradation of protein to ammonia were determined in a mixed rumen microbial population taken from sheep on a grass hay-concentrate diet. Both compounds decreased NH3 production by inhibiting deamination of amino acids. LY29, but not DIC, inhibited growth of the high-activity ammonia-producing species, Clostridium aminophilum and Clostridium sticklandii.

KW - Animal Feed

KW - Animals

KW - Azetidines/pharmacology

KW - Bacteria/drug effects

KW - Biphenyl Compounds/pharmacology

KW - Clostridium/drug effects

KW - Deamination

KW - Endopeptidases/metabolism

KW - Enzyme Inhibitors

KW - Onium Compounds/pharmacology

KW - Poaceae

KW - Proline/analogs & derivatives

KW - Proteins/metabolism

KW - Pyrrolidines/pharmacology

KW - Rumen/metabolism

KW - Sheep

M3 - Article

C2 - 10388733

VL - 65

SP - 3258

EP - 3260

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

SN - 0099-2240

IS - 7

ER -