Influence of 1-[(E)-2-(2-methyl-4-nitrophenyl)diaz-1-enyl]pyrrolidine-2-carboxylic acid and diphenyliodonium chloride on ruminal protein metabolism and ruminal microorganisms

F Floret, L C Chaudhary, W C Ellis, S El Hassan, N McKain, C J Newbold, R J Wallace

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The effects of 1-[(E)-2-(2-methyl-4-nitrophenyl)diaz-1-enyl]pyrrolidine-2-carboxy lic acid (LY29) and diphenyliodonium chloride (DIC) on the degradation of protein to ammonia were determined in a mixed rumen microbial population taken from sheep on a grass hay-concentrate diet. Both compounds decreased NH3 production by inhibiting deamination of amino acids. LY29, but not DIC, inhibited growth of the high-activity ammonia-producing species, Clostridium aminophilum and Clostridium sticklandii.

Original languageEnglish
Pages (from-to)3258-60
Number of pages3
JournalApplied and Environmental Microbiology
Issue number7
Publication statusPrint publication - Jul 1999
Externally publishedYes



  • Animal Feed
  • Animals
  • Azetidines/pharmacology
  • Bacteria/drug effects
  • Biphenyl Compounds/pharmacology
  • Clostridium/drug effects
  • Deamination
  • Endopeptidases/metabolism
  • Enzyme Inhibitors
  • Onium Compounds/pharmacology
  • Poaceae
  • Proline/analogs & derivatives
  • Proteins/metabolism
  • Pyrrolidines/pharmacology
  • Rumen/metabolism
  • Sheep

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