Influence of 1-[(E)-2-(2-methyl-4-nitrophenyl)diaz-1-enyl]pyrrolidine-2-carboxylic acid and diphenyliodonium chloride on ruminal protein metabolism and ruminal microorganisms

F Floret; L C Chaudhary; W C Ellis; S El Hassan; N McKain; C J Newbold; R J Wallace

Influence of 1-[(E)-2-(2-methyl-4-nitrophenyl)diaz-1-enyl]pyrrolidine-2-carboxylic acid and diphenyliodonium chloride on ruminal protein metabolism and ruminal microorganisms

F Floret, L C Chaudhary, W C Ellis, S El Hassan, N McKain, C J Newbold, R J Wallace

Research output: Contribution to journal › Article › peer-review

Abstract

The effects of 1-[(E)-2-(2-methyl-4-nitrophenyl)diaz-1-enyl]pyrrolidine-2-carboxy lic acid (LY29) and diphenyliodonium chloride (DIC) on the degradation of protein to ammonia were determined in a mixed rumen microbial population taken from sheep on a grass hay-concentrate diet. Both compounds decreased NH3 production by inhibiting deamination of amino acids. LY29, but not DIC, inhibited growth of the high-activity ammonia-producing species, Clostridium aminophilum and Clostridium sticklandii.

Original language	English
Pages (from-to)	3258-60
Number of pages	3
Journal	Applied and Environmental Microbiology
Volume	65
Issue number	7
Publication status	Print publication - Jul 1999
Externally published	Yes

Keywords

Animal Feed
Animals
Azetidines/pharmacology
Bacteria/drug effects
Biphenyl Compounds/pharmacology
Clostridium/drug effects
Deamination
Endopeptidases/metabolism
Enzyme Inhibitors
Onium Compounds/pharmacology
Poaceae
Proline/analogs & derivatives
Proteins/metabolism
Pyrrolidines/pharmacology
Rumen/metabolism
Sheep

Cite this

@article{992510fb2ec74b1db87e6be3ce300fef,

title = "Influence of 1-[(E)-2-(2-methyl-4-nitrophenyl)diaz-1-enyl]pyrrolidine-2-carboxylic acid and diphenyliodonium chloride on ruminal protein metabolism and ruminal microorganisms",

abstract = "The effects of 1-[(E)-2-(2-methyl-4-nitrophenyl)diaz-1-enyl]pyrrolidine-2-carboxy lic acid (LY29) and diphenyliodonium chloride (DIC) on the degradation of protein to ammonia were determined in a mixed rumen microbial population taken from sheep on a grass hay-concentrate diet. Both compounds decreased NH3 production by inhibiting deamination of amino acids. LY29, but not DIC, inhibited growth of the high-activity ammonia-producing species, Clostridium aminophilum and Clostridium sticklandii.",

keywords = "Animal Feed, Animals, Azetidines/pharmacology, Bacteria/drug effects, Biphenyl Compounds/pharmacology, Clostridium/drug effects, Deamination, Endopeptidases/metabolism, Enzyme Inhibitors, Onium Compounds/pharmacology, Poaceae, Proline/analogs \& derivatives, Proteins/metabolism, Pyrrolidines/pharmacology, Rumen/metabolism, Sheep",

author = "F Floret and Chaudhary, \{L C\} and Ellis, \{W C\} and \{El Hassan\}, S and N McKain and Newbold, \{C J\} and Wallace, \{R J\}",

year = "1999",

month = jul,

language = "English",

volume = "65",

pages = "3258--60",

journal = "Applied and Environmental Microbiology",

issn = "0099-2240",

publisher = "American Society for Microbiology",

number = "7",

}

Influence of 1-[(E)-2-(2-methyl-4-nitrophenyl)diaz-1-enyl]pyrrolidine-2-carboxylic acid and diphenyliodonium chloride on ruminal protein metabolism and ruminal microorganisms. / Floret, F; Chaudhary, L C; Ellis, W C et al.
In: Applied and Environmental Microbiology, Vol. 65, No. 7, 07.1999, p. 3258-60.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Influence of 1-[(E)-2-(2-methyl-4-nitrophenyl)diaz-1-enyl]pyrrolidine-2-carboxylic acid and diphenyliodonium chloride on ruminal protein metabolism and ruminal microorganisms

AU - Floret, F

AU - Chaudhary, L C

AU - Ellis, W C

AU - El Hassan, S

AU - McKain, N

AU - Newbold, C J

AU - Wallace, R J

PY - 1999/7

Y1 - 1999/7

N2 - The effects of 1-[(E)-2-(2-methyl-4-nitrophenyl)diaz-1-enyl]pyrrolidine-2-carboxy lic acid (LY29) and diphenyliodonium chloride (DIC) on the degradation of protein to ammonia were determined in a mixed rumen microbial population taken from sheep on a grass hay-concentrate diet. Both compounds decreased NH3 production by inhibiting deamination of amino acids. LY29, but not DIC, inhibited growth of the high-activity ammonia-producing species, Clostridium aminophilum and Clostridium sticklandii.

AB - The effects of 1-[(E)-2-(2-methyl-4-nitrophenyl)diaz-1-enyl]pyrrolidine-2-carboxy lic acid (LY29) and diphenyliodonium chloride (DIC) on the degradation of protein to ammonia were determined in a mixed rumen microbial population taken from sheep on a grass hay-concentrate diet. Both compounds decreased NH3 production by inhibiting deamination of amino acids. LY29, but not DIC, inhibited growth of the high-activity ammonia-producing species, Clostridium aminophilum and Clostridium sticklandii.

KW - Animal Feed

KW - Animals

KW - Azetidines/pharmacology

KW - Bacteria/drug effects

KW - Biphenyl Compounds/pharmacology

KW - Clostridium/drug effects

KW - Deamination

KW - Endopeptidases/metabolism

KW - Enzyme Inhibitors

KW - Onium Compounds/pharmacology

KW - Poaceae

KW - Proline/analogs & derivatives

KW - Proteins/metabolism

KW - Pyrrolidines/pharmacology

KW - Rumen/metabolism

KW - Sheep

M3 - Article

C2 - 10388733

SN - 0099-2240

VL - 65

SP - 3258

EP - 3260

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 7

ER -

Influence of 1-[(E)-2-(2-methyl-4-nitrophenyl)diaz-1-enyl]pyrrolidine-2-carboxylic acid and diphenyliodonium chloride on ruminal protein metabolism and ruminal microorganisms

Abstract

Keywords

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Cite this