Influence of chilling stress on the intercellular distribution of assimilatory sulfate reduction and thiols in Zea mays

S. Kopriva*, S. Jones, A. Koprivova, M. Suter, P. Von Ballmoos, K. Brander, J. Flückiger, C. Brunold

*Corresponding author for this work

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

The effect of chilling on the intercellular distribution of mRNAs for enzymes of assimilatory sulfate reduction, the activity of adenosine 5′-phosphosulfate reductase (APR), and the level of glutathione was analysed in leaves and roots of maize (Zea mays L). At 25°C the mRNAs for APR, ATP sulfurylase, and sulfite reductase accumulated in bundle-sheath only, whereas the mRNA for O-acetylserine sulfhydrylase was also detected in mesophyll cells. Glutathione was predominantly detected in mesophyll cells; however, oxidized glutathione was equally distributed between the two cell types. Chilling at 12°C induced oxidative stress which resulted in increased concentrations of oxidized glutathione in both cell types and a prominent increase of APR mRNA and activity in bundle-sheath cells. After chilling, mRNAs for APR and sulfite reductase, as well as low APR activity, were detected in mesophyll cells. In roots, APR mRNA and activity were at higher levels in root tips than in the mature root and were greatly increased after chilling. These results demonstrate that chilling stress affected the levels and the intercellular distribution of mRNAs for enzymes of sulfate assimilation.

Original languageEnglish
Pages (from-to)24-31
Number of pages8
JournalPlant Biology
Volume3
Issue number1
DOIs
Publication statusPrint publication - 1 Jan 2001
Externally publishedYes

Keywords

  • C photosynthesis
  • Cysteine biosynthesis
  • Glutathione
  • In situ RNA hybridization
  • Maize
  • Oxidative stress
  • Sulfate assimilation

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    Kopriva, S., Jones, S., Koprivova, A., Suter, M., Von Ballmoos, P., Brander, K., Flückiger, J., & Brunold, C. (2001). Influence of chilling stress on the intercellular distribution of assimilatory sulfate reduction and thiols in Zea mays. Plant Biology, 3(1), 24-31. https://doi.org/10.1055/s-2001-11745