Influence of nitrogen source on the fermentation of fibre from barley straw and sugarbeet pulp by ruminal micro-organisms in vitro

M J Ranilla, M D Carro, S López, C J Newbold, R J Wallace

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Incubations were carried out with a batch culture system to study the effects of different N sources on the fermentation by ruminal micro-organisms from Merino sheep of two fibre substrates derived from feedstuffs that differed in their fermentation rate. The substrates were neutral-detergent fibre (NDF) from barley straw and sugarbeet pulp. N sources were ammonia (NH4Cl) and peptides (Trypticase). Three treatments were made by replacing ammonia-N with peptide-N at levels of 0 (AMMO), 33 (PEPLOW) and 66 % (PEPHIGH) of total N. There were no differences (P>0.05) between treatments in NDF degradation for both the barley straw and the sugarbeet pulp. Peptides increased (P<0.05) total volatile fatty acids daily production for both substrates, with greater values (P<0.001) for PEPHIGH than for PEPLOW for the sugarbeet pulp. The presence of peptides also increased (P<0.05) microbial N synthesis compared with AMMO, with PEPHIGH supporting more growth (P<0.001) than PEPLOW when the sugarbeet pulp NDF was fermented. The presence of peptides increased (P<0.01) the amount of solids-associated micro-organisms (SAM)-N for both the barley straw and the sugarbeet pulp fibres, values in the PEPHIGH treatment being higher (P<0.001) than those in PEPLOW. The proportion of SAM-N in the total microbial N was not affected (P>0.05) by the presence of peptides compared with the AMMO treatment, but values were greater for the PEPHIGH compared with the PEPLOW N source, reaching statistical significance (P<0.05) only for the sugarbeet pulp. For liquid-associated micro-organisms, the AMMO treatment resulted in the greatest (P<0.05) proportion of N derived from ammonia for both substrates, with a further decrease (P<0.01) for the PEPHIGH treatment compared with the PEPLOW for the sugarbeet pulp, indicating preferential uptake of peptides when they were available. Microbial growth efficiency (g microbial N/kg NDF degraded) was not affected (P>0.05) by N source. These results indicate that N forms other than ammonia are needed for maximal growth of fibre-digesting ruminal micro-organisms.

Original languageEnglish
Pages (from-to)717-24
Number of pages8
JournalBritish Journal of Nutrition
Volume86
Issue number6
Publication statusPrint publication - Dec 2001
Externally publishedYes

Fingerprint

Hordeum
Fermentation
Nitrogen
Ammonia
Peptides
Detergents
Batch Cell Culture Techniques
Sheep
In Vitro Techniques
Growth

Keywords

  • Animal Feed
  • Animal Nutritional Physiological Phenomena
  • Animals
  • Bacteria/metabolism
  • Bacterial Proteins/biosynthesis
  • Dietary Fiber/metabolism
  • Fermentation/physiology
  • Hordeum
  • Nitrogen/administration & dosage
  • Rumen/metabolism
  • Sheep/metabolism

Cite this

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title = "Influence of nitrogen source on the fermentation of fibre from barley straw and sugarbeet pulp by ruminal micro-organisms in vitro",
abstract = "Incubations were carried out with a batch culture system to study the effects of different N sources on the fermentation by ruminal micro-organisms from Merino sheep of two fibre substrates derived from feedstuffs that differed in their fermentation rate. The substrates were neutral-detergent fibre (NDF) from barley straw and sugarbeet pulp. N sources were ammonia (NH4Cl) and peptides (Trypticase). Three treatments were made by replacing ammonia-N with peptide-N at levels of 0 (AMMO), 33 (PEPLOW) and 66 {\%} (PEPHIGH) of total N. There were no differences (P>0.05) between treatments in NDF degradation for both the barley straw and the sugarbeet pulp. Peptides increased (P<0.05) total volatile fatty acids daily production for both substrates, with greater values (P<0.001) for PEPHIGH than for PEPLOW for the sugarbeet pulp. The presence of peptides also increased (P<0.05) microbial N synthesis compared with AMMO, with PEPHIGH supporting more growth (P<0.001) than PEPLOW when the sugarbeet pulp NDF was fermented. The presence of peptides increased (P<0.01) the amount of solids-associated micro-organisms (SAM)-N for both the barley straw and the sugarbeet pulp fibres, values in the PEPHIGH treatment being higher (P<0.001) than those in PEPLOW. The proportion of SAM-N in the total microbial N was not affected (P>0.05) by the presence of peptides compared with the AMMO treatment, but values were greater for the PEPHIGH compared with the PEPLOW N source, reaching statistical significance (P<0.05) only for the sugarbeet pulp. For liquid-associated micro-organisms, the AMMO treatment resulted in the greatest (P<0.05) proportion of N derived from ammonia for both substrates, with a further decrease (P<0.01) for the PEPHIGH treatment compared with the PEPLOW for the sugarbeet pulp, indicating preferential uptake of peptides when they were available. Microbial growth efficiency (g microbial N/kg NDF degraded) was not affected (P>0.05) by N source. These results indicate that N forms other than ammonia are needed for maximal growth of fibre-digesting ruminal micro-organisms.",
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Influence of nitrogen source on the fermentation of fibre from barley straw and sugarbeet pulp by ruminal micro-organisms in vitro. / Ranilla, M J; Carro, M D; López, S; Newbold, C J; Wallace, R J.

In: British Journal of Nutrition, Vol. 86, No. 6, 12.2001, p. 717-24.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Influence of nitrogen source on the fermentation of fibre from barley straw and sugarbeet pulp by ruminal micro-organisms in vitro

AU - Ranilla, M J

AU - Carro, M D

AU - López, S

AU - Newbold, C J

AU - Wallace, R J

PY - 2001/12

Y1 - 2001/12

N2 - Incubations were carried out with a batch culture system to study the effects of different N sources on the fermentation by ruminal micro-organisms from Merino sheep of two fibre substrates derived from feedstuffs that differed in their fermentation rate. The substrates were neutral-detergent fibre (NDF) from barley straw and sugarbeet pulp. N sources were ammonia (NH4Cl) and peptides (Trypticase). Three treatments were made by replacing ammonia-N with peptide-N at levels of 0 (AMMO), 33 (PEPLOW) and 66 % (PEPHIGH) of total N. There were no differences (P>0.05) between treatments in NDF degradation for both the barley straw and the sugarbeet pulp. Peptides increased (P<0.05) total volatile fatty acids daily production for both substrates, with greater values (P<0.001) for PEPHIGH than for PEPLOW for the sugarbeet pulp. The presence of peptides also increased (P<0.05) microbial N synthesis compared with AMMO, with PEPHIGH supporting more growth (P<0.001) than PEPLOW when the sugarbeet pulp NDF was fermented. The presence of peptides increased (P<0.01) the amount of solids-associated micro-organisms (SAM)-N for both the barley straw and the sugarbeet pulp fibres, values in the PEPHIGH treatment being higher (P<0.001) than those in PEPLOW. The proportion of SAM-N in the total microbial N was not affected (P>0.05) by the presence of peptides compared with the AMMO treatment, but values were greater for the PEPHIGH compared with the PEPLOW N source, reaching statistical significance (P<0.05) only for the sugarbeet pulp. For liquid-associated micro-organisms, the AMMO treatment resulted in the greatest (P<0.05) proportion of N derived from ammonia for both substrates, with a further decrease (P<0.01) for the PEPHIGH treatment compared with the PEPLOW for the sugarbeet pulp, indicating preferential uptake of peptides when they were available. Microbial growth efficiency (g microbial N/kg NDF degraded) was not affected (P>0.05) by N source. These results indicate that N forms other than ammonia are needed for maximal growth of fibre-digesting ruminal micro-organisms.

AB - Incubations were carried out with a batch culture system to study the effects of different N sources on the fermentation by ruminal micro-organisms from Merino sheep of two fibre substrates derived from feedstuffs that differed in their fermentation rate. The substrates were neutral-detergent fibre (NDF) from barley straw and sugarbeet pulp. N sources were ammonia (NH4Cl) and peptides (Trypticase). Three treatments were made by replacing ammonia-N with peptide-N at levels of 0 (AMMO), 33 (PEPLOW) and 66 % (PEPHIGH) of total N. There were no differences (P>0.05) between treatments in NDF degradation for both the barley straw and the sugarbeet pulp. Peptides increased (P<0.05) total volatile fatty acids daily production for both substrates, with greater values (P<0.001) for PEPHIGH than for PEPLOW for the sugarbeet pulp. The presence of peptides also increased (P<0.05) microbial N synthesis compared with AMMO, with PEPHIGH supporting more growth (P<0.001) than PEPLOW when the sugarbeet pulp NDF was fermented. The presence of peptides increased (P<0.01) the amount of solids-associated micro-organisms (SAM)-N for both the barley straw and the sugarbeet pulp fibres, values in the PEPHIGH treatment being higher (P<0.001) than those in PEPLOW. The proportion of SAM-N in the total microbial N was not affected (P>0.05) by the presence of peptides compared with the AMMO treatment, but values were greater for the PEPHIGH compared with the PEPLOW N source, reaching statistical significance (P<0.05) only for the sugarbeet pulp. For liquid-associated micro-organisms, the AMMO treatment resulted in the greatest (P<0.05) proportion of N derived from ammonia for both substrates, with a further decrease (P<0.01) for the PEPHIGH treatment compared with the PEPLOW for the sugarbeet pulp, indicating preferential uptake of peptides when they were available. Microbial growth efficiency (g microbial N/kg NDF degraded) was not affected (P>0.05) by N source. These results indicate that N forms other than ammonia are needed for maximal growth of fibre-digesting ruminal micro-organisms.

KW - Animal Feed

KW - Animal Nutritional Physiological Phenomena

KW - Animals

KW - Bacteria/metabolism

KW - Bacterial Proteins/biosynthesis

KW - Dietary Fiber/metabolism

KW - Fermentation/physiology

KW - Hordeum

KW - Nitrogen/administration & dosage

KW - Rumen/metabolism

KW - Sheep/metabolism

M3 - Article

C2 - 11749681

VL - 86

SP - 717

EP - 724

JO - British Journal of Nutrition

JF - British Journal of Nutrition

SN - 0007-1145

IS - 6

ER -