Inhibition and kinetic studies of lignin degrading enzymes of Ganoderma boninense by naturally occurring phenolic compounds

A Surendran, Y Siddiqui, H M Saud, N S Ali, S Manickam

Research output: Contribution to journalArticlepeer-review

21 Citations (Scopus)

Abstract

AIM: Lignolytic (lignin degrading) enzyme, from oil palm pathogen Ganoderma boninense Pat. (Syn G. orbiforme (Ryvarden)), is involved in the detoxification and the degradation of lignin in the oil palm and is the rate-limiting step in the infection process of this fungus. Active inhibition of lignin-degrading enzymes secreted by G. boninense by various naturally occurring phenolic compounds and estimation of efficiency on pathogen suppression was aimed at.

METHODS AND RESULTS: In our work, 10 naturally occurring phenolic compounds were evaluated for their inhibitory potential towards the lignolytic enzymes of G. boninense. Additionally, the lignin-degrading enzymes were characterized. Most of the peholic compounds exhibited an uncompetitive inhibition towards the lignin-degrading enzymes. Benzoic acid was the superior inhibitor to the production of lignin-degrading enzymes, when compared between the 10 phenolic compounds. The inhibitory potential of the phenolic compounds towards the lignin-degrading enzymes are higher than that of the conventional metal ion inhibitor. The lignin-degrading enzymes were stable in a wide range of pH but were sensitive to higher temperature.

CONCLUSION: The study demonstrated the inhibitor potential of 10 naturally occurring phenolic compounds towards the lignin-degrading enzymes of G. boninense with different efficacies.

SIGNIFICANCE AND IMPACT OF THE STUDY: The study has shed a light towards a new management strategy to control basal stem rot disease in oil palm. It serves as a replacement for the existing chemical control.

Original languageEnglish
Pages (from-to)876-887
Number of pages12
JournalJournal of Applied Microbiology
Volume125
Issue number3
DOIs
Publication statusPrint publication - Sept 2018
Externally publishedYes

Bibliographical note

© 2018 The Society for Applied Microbiology.

Keywords

  • Cellulases/antagonists & inhibitors
  • Fungal Proteins/antagonists & inhibitors
  • Ganoderma/drug effects
  • Kinetics
  • Lignin/metabolism
  • Phenols/pharmacology

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