Insecticide resistance status of Myzus persicae in Greece: long-term surveys and new diagnostics for resistance mechanisms

Costas Ch Voudouris, Amalia N Kati, Eldem Sadikoglou, Martin Williamson, Panagiotis J Skouras, Ourania Dimotsiou, Stella Georgiou, Brian Fenton, George Skavdis, John T Margaritopoulos

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Abstract

BACKGROUND: Myzus persicae nicotianae is an important pest in Greece, controlled mainly by neonicotinoids. Monitoring of the aphid populations for resistance mechanisms is essential for effective control.

RESULTS: Two new RFLP-based diagnostics for the detection of the M918T (super-kdr pyrethroid resistance) and nAChR R81T (neonicotinoid resistance) mutations were applied, along with other established assays, on 131 nicotianae multilocus genotypes (MLGs) collected from tobacco and peach in Greece in 2012-2013. Furthermore, we present resistance data from aphid clones (>500, mainly nicotianae) collected in 2006-2007. About half of the clones tested with a diagnostic dose of imidacloprid were tolerant. The R81T mutation was not found in the 131 MLGs and 152 clones examined. Over half (58.6%) of a subset of 29 clones showed a 9-36-fold overexpression of CYP6CY3. M918T was found at low to moderate frequencies. The kdr and MACE mechanisms and carboxylesterase-based resistance were found at high frequency in all years.

CONCLUSION: The aphid retains costly resistance mechanisms even in the absence of pressure from certain insecticides, which could be attributed to factors related to climate and genetic properties of the populations. The indication of build-up of resistance/tolerance to neonicotinoids, related to CYP6CY3 overexpression, is a matter of concern. © 2015 Society of Chemical Industry.

Original languageEnglish
Pages (from-to)671-83
Number of pages13
JournalPest Management Science
Volume72
Issue number4
Early online date29 May 2015
DOIs
Publication statusPrint publication - Apr 2016

Bibliographical note

© 2015 Society of Chemical Industry.

Keywords

  • Animals
  • Aphids/drug effects
  • Carboxylesterase/genetics
  • Genetic Loci/genetics
  • Genotype
  • Greece
  • Imidazoles
  • Insecticide Resistance/genetics
  • Linkage Disequilibrium
  • Mutation
  • Neonicotinoids
  • Nitro Compounds
  • Polymerase Chain Reaction
  • Polymorphism, Restriction Fragment Length
  • Prunus persica
  • Surveys and Questionnaires
  • Tobacco

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  • Cite this

    Voudouris, C. C., Kati, A. N., Sadikoglou, E., Williamson, M., Skouras, P. J., Dimotsiou, O., Georgiou, S., Fenton, B., Skavdis, G., & Margaritopoulos, J. T. (2016). Insecticide resistance status of Myzus persicae in Greece: long-term surveys and new diagnostics for resistance mechanisms. Pest Management Science, 72(4), 671-83. https://doi.org/10.1002/ps.4036