Insecticide resistance status of Myzus persicae in Greece

long-term surveys and new diagnostics for resistance mechanisms

Costas Ch Voudouris, Amalia N Kati, Eldem Sadikoglou, Martin Williamson, Panagiotis J Skouras, Ourania Dimotsiou, Stella Georgiou, Brian Fenton, George Skavdis, John T Margaritopoulos

Research output: Contribution to journalArticleResearchpeer-review

17 Citations (Scopus)

Abstract

BACKGROUND: Myzus persicae nicotianae is an important pest in Greece, controlled mainly by neonicotinoids. Monitoring of the aphid populations for resistance mechanisms is essential for effective control.

RESULTS: Two new RFLP-based diagnostics for the detection of the M918T (super-kdr pyrethroid resistance) and nAChR R81T (neonicotinoid resistance) mutations were applied, along with other established assays, on 131 nicotianae multilocus genotypes (MLGs) collected from tobacco and peach in Greece in 2012-2013. Furthermore, we present resistance data from aphid clones (>500, mainly nicotianae) collected in 2006-2007. About half of the clones tested with a diagnostic dose of imidacloprid were tolerant. The R81T mutation was not found in the 131 MLGs and 152 clones examined. Over half (58.6%) of a subset of 29 clones showed a 9-36-fold overexpression of CYP6CY3. M918T was found at low to moderate frequencies. The kdr and MACE mechanisms and carboxylesterase-based resistance were found at high frequency in all years.

CONCLUSION: The aphid retains costly resistance mechanisms even in the absence of pressure from certain insecticides, which could be attributed to factors related to climate and genetic properties of the populations. The indication of build-up of resistance/tolerance to neonicotinoids, related to CYP6CY3 overexpression, is a matter of concern. © 2015 Society of Chemical Industry.

Original languageEnglish
Pages (from-to)671-83
Number of pages13
JournalPest Management Science
Volume72
Issue number4
Early online date29 May 2015
DOIs
Publication statusPrint publication - Apr 2016

Fingerprint

insecticide resistance
Myzus persicae
resistance mechanisms
Greece
neonicotinoid insecticides
clones
Aphidoidea
Myzus persicae nicotianae
mutation
carboxylesterase
genotype
imidacloprid
pyrethrins
peaches
restriction fragment length polymorphism
tobacco
insecticides
pests
climate
monitoring

Bibliographical note

© 2015 Society of Chemical Industry.

Keywords

  • Animals
  • Aphids/drug effects
  • Carboxylesterase/genetics
  • Genetic Loci/genetics
  • Genotype
  • Greece
  • Imidazoles
  • Insecticide Resistance/genetics
  • Linkage Disequilibrium
  • Mutation
  • Neonicotinoids
  • Nitro Compounds
  • Polymerase Chain Reaction
  • Polymorphism, Restriction Fragment Length
  • Prunus persica
  • Surveys and Questionnaires
  • Tobacco

Cite this

Voudouris, C. C., Kati, A. N., Sadikoglou, E., Williamson, M., Skouras, P. J., Dimotsiou, O., ... Margaritopoulos, J. T. (2016). Insecticide resistance status of Myzus persicae in Greece: long-term surveys and new diagnostics for resistance mechanisms. Pest Management Science, 72(4), 671-83. https://doi.org/10.1002/ps.4036
Voudouris, Costas Ch ; Kati, Amalia N ; Sadikoglou, Eldem ; Williamson, Martin ; Skouras, Panagiotis J ; Dimotsiou, Ourania ; Georgiou, Stella ; Fenton, Brian ; Skavdis, George ; Margaritopoulos, John T. / Insecticide resistance status of Myzus persicae in Greece : long-term surveys and new diagnostics for resistance mechanisms. In: Pest Management Science. 2016 ; Vol. 72, No. 4. pp. 671-83.
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title = "Insecticide resistance status of Myzus persicae in Greece: long-term surveys and new diagnostics for resistance mechanisms",
abstract = "BACKGROUND: Myzus persicae nicotianae is an important pest in Greece, controlled mainly by neonicotinoids. Monitoring of the aphid populations for resistance mechanisms is essential for effective control.RESULTS: Two new RFLP-based diagnostics for the detection of the M918T (super-kdr pyrethroid resistance) and nAChR R81T (neonicotinoid resistance) mutations were applied, along with other established assays, on 131 nicotianae multilocus genotypes (MLGs) collected from tobacco and peach in Greece in 2012-2013. Furthermore, we present resistance data from aphid clones (>500, mainly nicotianae) collected in 2006-2007. About half of the clones tested with a diagnostic dose of imidacloprid were tolerant. The R81T mutation was not found in the 131 MLGs and 152 clones examined. Over half (58.6{\%}) of a subset of 29 clones showed a 9-36-fold overexpression of CYP6CY3. M918T was found at low to moderate frequencies. The kdr and MACE mechanisms and carboxylesterase-based resistance were found at high frequency in all years.CONCLUSION: The aphid retains costly resistance mechanisms even in the absence of pressure from certain insecticides, which could be attributed to factors related to climate and genetic properties of the populations. The indication of build-up of resistance/tolerance to neonicotinoids, related to CYP6CY3 overexpression, is a matter of concern. {\circledC} 2015 Society of Chemical Industry.",
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author = "Voudouris, {Costas Ch} and Kati, {Amalia N} and Eldem Sadikoglou and Martin Williamson and Skouras, {Panagiotis J} and Ourania Dimotsiou and Stella Georgiou and Brian Fenton and George Skavdis and Margaritopoulos, {John T}",
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Voudouris, CC, Kati, AN, Sadikoglou, E, Williamson, M, Skouras, PJ, Dimotsiou, O, Georgiou, S, Fenton, B, Skavdis, G & Margaritopoulos, JT 2016, 'Insecticide resistance status of Myzus persicae in Greece: long-term surveys and new diagnostics for resistance mechanisms', Pest Management Science, vol. 72, no. 4, pp. 671-83. https://doi.org/10.1002/ps.4036

Insecticide resistance status of Myzus persicae in Greece : long-term surveys and new diagnostics for resistance mechanisms. / Voudouris, Costas Ch; Kati, Amalia N; Sadikoglou, Eldem; Williamson, Martin; Skouras, Panagiotis J; Dimotsiou, Ourania; Georgiou, Stella; Fenton, Brian; Skavdis, George; Margaritopoulos, John T.

In: Pest Management Science, Vol. 72, No. 4, 04.2016, p. 671-83.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Insecticide resistance status of Myzus persicae in Greece

T2 - long-term surveys and new diagnostics for resistance mechanisms

AU - Voudouris, Costas Ch

AU - Kati, Amalia N

AU - Sadikoglou, Eldem

AU - Williamson, Martin

AU - Skouras, Panagiotis J

AU - Dimotsiou, Ourania

AU - Georgiou, Stella

AU - Fenton, Brian

AU - Skavdis, George

AU - Margaritopoulos, John T

N1 - © 2015 Society of Chemical Industry.

PY - 2016/4

Y1 - 2016/4

N2 - BACKGROUND: Myzus persicae nicotianae is an important pest in Greece, controlled mainly by neonicotinoids. Monitoring of the aphid populations for resistance mechanisms is essential for effective control.RESULTS: Two new RFLP-based diagnostics for the detection of the M918T (super-kdr pyrethroid resistance) and nAChR R81T (neonicotinoid resistance) mutations were applied, along with other established assays, on 131 nicotianae multilocus genotypes (MLGs) collected from tobacco and peach in Greece in 2012-2013. Furthermore, we present resistance data from aphid clones (>500, mainly nicotianae) collected in 2006-2007. About half of the clones tested with a diagnostic dose of imidacloprid were tolerant. The R81T mutation was not found in the 131 MLGs and 152 clones examined. Over half (58.6%) of a subset of 29 clones showed a 9-36-fold overexpression of CYP6CY3. M918T was found at low to moderate frequencies. The kdr and MACE mechanisms and carboxylesterase-based resistance were found at high frequency in all years.CONCLUSION: The aphid retains costly resistance mechanisms even in the absence of pressure from certain insecticides, which could be attributed to factors related to climate and genetic properties of the populations. The indication of build-up of resistance/tolerance to neonicotinoids, related to CYP6CY3 overexpression, is a matter of concern. © 2015 Society of Chemical Industry.

AB - BACKGROUND: Myzus persicae nicotianae is an important pest in Greece, controlled mainly by neonicotinoids. Monitoring of the aphid populations for resistance mechanisms is essential for effective control.RESULTS: Two new RFLP-based diagnostics for the detection of the M918T (super-kdr pyrethroid resistance) and nAChR R81T (neonicotinoid resistance) mutations were applied, along with other established assays, on 131 nicotianae multilocus genotypes (MLGs) collected from tobacco and peach in Greece in 2012-2013. Furthermore, we present resistance data from aphid clones (>500, mainly nicotianae) collected in 2006-2007. About half of the clones tested with a diagnostic dose of imidacloprid were tolerant. The R81T mutation was not found in the 131 MLGs and 152 clones examined. Over half (58.6%) of a subset of 29 clones showed a 9-36-fold overexpression of CYP6CY3. M918T was found at low to moderate frequencies. The kdr and MACE mechanisms and carboxylesterase-based resistance were found at high frequency in all years.CONCLUSION: The aphid retains costly resistance mechanisms even in the absence of pressure from certain insecticides, which could be attributed to factors related to climate and genetic properties of the populations. The indication of build-up of resistance/tolerance to neonicotinoids, related to CYP6CY3 overexpression, is a matter of concern. © 2015 Society of Chemical Industry.

KW - Animals

KW - Aphids/drug effects

KW - Carboxylesterase/genetics

KW - Genetic Loci/genetics

KW - Genotype

KW - Greece

KW - Imidazoles

KW - Insecticide Resistance/genetics

KW - Linkage Disequilibrium

KW - Mutation

KW - Neonicotinoids

KW - Nitro Compounds

KW - Polymerase Chain Reaction

KW - Polymorphism, Restriction Fragment Length

KW - Prunus persica

KW - Surveys and Questionnaires

KW - Tobacco

U2 - 10.1002/ps.4036

DO - 10.1002/ps.4036

M3 - Article

VL - 72

SP - 671

EP - 683

JO - Pest Management Science

JF - Pest Management Science

SN - 1526-498X

IS - 4

ER -