Isolation and molecular characterization of entomopathogenic nematode, Heterorhabditis sp. from an arable land in Nigeria

Fisayo Y. DARAMOLA*, Osarenkhoe O. OSEMWEGIE, Stephen O. OWA, Samuel B. ORISAJO, Evbuomwan IKPONMWOSA, Elizabeth T. ALORI

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)


The occurrence of entomopathogenic nematodes (EPNs) in arable soil samples from Nigeria was investigated using Baermann extraction tray and insect-bait (White's trap) techniques. Isolates were tested for infectivity using the larvae of Galleria mellonella (greater moth) and Tenebrio molitor (mealworm). The study revealed a new species of Heterorhabditis (MT371593) in soil samples that were randomly collected from an arable farmland cultivated with cassava TMS-30572 at the Teaching and Research Farm of Landmark University, Nigeria. Amplification of the internal transcribed spacer region (ITS) of the ribosomal DNA produced a nucleotide sequence of 933 base pairs (bp). A BLASTN search of GenBank showed that the sequence of the Nigerian isolate is identical at 99% similarity to that of Heterorhabditis sp. from Thailand. Infectivity test of the isolate showed 100% mortality against T. molitor larvae within 48 h of exposure while only 80% mortality was recorded for G. mellonella after 1 week of exposure. This is the first account of Heterorhabditis sp. in Nigeria. The varying degrees of infectivity against mealworm and greater moth observed in this study proved that the Nigerian isolate of Heterorhabditis sp. could potentially be an attractive option in the management of insect pests of cash crops.

Original languageEnglish
Pages (from-to)2706-2715
Number of pages10
JournalJournal of Integrative Agriculture
Issue number10
Publication statusPrint publication - Oct 2021
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2021 CAAS. Publishing services by Elsevier B.V


  • biological control
  • EPNs
  • Heterorhabditis sp.
  • ribosomal DNA
  • Tenebrio molitor


Dive into the research topics of 'Isolation and molecular characterization of entomopathogenic nematode, Heterorhabditis sp. from an arable land in Nigeria'. Together they form a unique fingerprint.

Cite this