Isothermal amplification of genetically modified DNA sequences directly from plant tissues lowers the barriers to high-throughput and field-based genotyping

David Lee, Maurizio La Mura, Theo Allnutt, Wayne Powell, Andy Greenland

Research output: Contribution to journalArticlepeer-review

10 Citations (Scopus)

Abstract

DNA extractions are a major cost for high-throughput genotyping. The loop-mediated isothermal amplification (LAMP) assay has been used for the detection of two genetically modified (GM) related sequences. The amplification of target DNA sequences from leaf and maize seed tissues prepared with minimum preparative treatment (disruption in water) demonstrates the ability of LAMP to work in conditions normally inhibitive to PCRs. The wide dynamic range of detection in these samples suggests that LAMP is highly sensitive even when the target is presented in such a crude form. LAMP offers a means of reducing genotyping costs as well as simplifying testing procedures.

Original languageEnglish
Pages (from-to)9400-2
Number of pages3
JournalJournal of Agricultural and Food Chemistry
Volume57
Issue number20
DOIs
Publication statusPrint publication - 28 Oct 2009
Externally publishedYes

Keywords

  • DNA, Plant/genetics
  • Genotype
  • High-Throughput Screening Assays/methods
  • Nucleic Acid Amplification Techniques/economics
  • Zea mays/genetics

Fingerprint Dive into the research topics of 'Isothermal amplification of genetically modified DNA sequences directly from plant tissues lowers the barriers to high-throughput and field-based genotyping'. Together they form a unique fingerprint.

Cite this