Mapping-by-sequencing identifies HvPHYTOCHROME C as a candidate gene for the early maturity 5 locus modulating the circadian clock and photoperiodic flowering in barley

Artem Pankin, Chiara Campoli, Xue Dong, Benjamin Kilian, Rajiv Sharma, Axel Himmelbach, Reena Saini, Seth J Davis, Nils Stein, Korbinian Schneeberger, Maria von Korff

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Phytochromes play an important role in light signaling and photoperiodic control of flowering time in plants. Here we propose that the red/far-red light photoreceptor HvPHYTOCHROME C (HvPHYC), carrying a mutation in a conserved region of the GAF domain, is a candidate underlying the early maturity 5 locus in barley (Hordeum vulgare L.). We fine mapped the gene using a mapping-by-sequencing approach applied on the whole-exome capture data from bulked early flowering segregants derived from a backcross of the Bowman(eam5) introgression line. We demonstrate that eam5 disrupts circadian expression of clock genes. Moreover, it interacts with the major photoperiod response gene Ppd-H1 to accelerate flowering under noninductive short days. Our results suggest that HvPHYC participates in transmission of light signals to the circadian clock and thus modulates light-dependent processes such as photoperiodic regulation of flowering.

Original languageEnglish
Pages (from-to)383-96
Number of pages14
JournalGenetics
Volume198
Issue number1
DOIs
Publication statusPrint publication - Sep 2014
Externally publishedYes

Bibliographical note

Copyright © 2014 by the Genetics Society of America.

Keywords

  • Circadian Clocks/genetics
  • Exome
  • Flowers/genetics
  • Genes, Plant
  • Hordeum/genetics
  • Inbreeding
  • Photoperiod
  • Physical Chromosome Mapping
  • Phytochrome/genetics
  • Plant Development/genetics
  • Plant Proteins/genetics
  • Quantitative Trait Loci

Fingerprint Dive into the research topics of 'Mapping-by-sequencing identifies HvPHYTOCHROME C as a candidate gene for the early maturity 5 locus modulating the circadian clock and photoperiodic flowering in barley'. Together they form a unique fingerprint.

  • Profiles

    No photo of Rajiv Sharma

    Rajiv Sharma

    Person: Academic contract that is research only

    Cite this

    Pankin, A., Campoli, C., Dong, X., Kilian, B., Sharma, R., Himmelbach, A., Saini, R., Davis, S. J., Stein, N., Schneeberger, K., & von Korff, M. (2014). Mapping-by-sequencing identifies HvPHYTOCHROME C as a candidate gene for the early maturity 5 locus modulating the circadian clock and photoperiodic flowering in barley. Genetics, 198(1), 383-96. https://doi.org/10.1534/genetics.114.165613