MicroRNA-20a in human faeces as a non-invasive biomarker for colorectal cancer

Tung On Yau; Chung Wah Wu; Ceen-Ming Tang; Yingxuan Chen; Jingyuan Fang; Yujuan Dong; Qiaoyi Liang; Simon Siu Man Ng; Francis Ka Leung Chan; Joseph Jao Yiu Sung; Jun Yu

doi:10.18632/oncotarget.6403

MicroRNA-20a in human faeces as a non-invasive biomarker for colorectal cancer

Tung On Yau
, Chung Wah Wu
, Ceen-Ming Tang
, Yingxuan Chen
, Jingyuan Fang
, Yujuan Dong
, Qiaoyi Liang
, Simon Siu Man Ng
, Francis Ka Leung Chan
, Joseph Jao Yiu Sung
, Jun Yu

Research output: Contribution to journal › Article › peer-review

60 Citations (Scopus)

Abstract

OBJECTIVE: Detection of microRNA (miRNA) aberrations in human faeces is a new approach for colorectal cancer (CRC) screening. The aim of this study was to characterise miR-20a in faeces as a non-invasive biomarker for diagnosis of CRC.

RESULTS: miR-20a expression was significantly higher in the 40 CRC tumours compared to their respective adjacent normal tissues (P = 0.0065). Levels of miR-20a were also significantly higher in faecal samples from CRC patients (P < 0.0001). The area under receiver operating characteristic (AUROC) curve for miR-20a was 0.73, with a sensitivity of 55% and specificity of 82% for CRC patients compared with controls. No significant difference in the level of miR-20a was found between patients with proximal, distal, and rectal cancer. The use of antibiotics did not influence faecal miR-20a levels.

PATIENTS AND METHODS: miR-20a was selected from an expression microarray containing 667 miRNAs. Further verification of miR-20a was performed in 40 pairs of primary CRC tissues, as well as 595 faecal samples (198 CRCs, 199 adenomas, and 198 healthy controls) using TaqMan probe based quantitative Real-Time PCR (qRT-PCR).

CONCLUSIONS: Faecal-based miR-20a can be utilised as a potential non-invasive biomarker for CRC screening.

Original language	English
Pages (from-to)	1559-68
Number of pages	10
Journal	Oncotarget
Volume	7
Issue number	2
DOIs	https://doi.org/10.18632/oncotarget.6403
Publication status	Print publication - 12 Jan 2016
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Adenoma/diagnosis
Aged
Area Under Curve
Biomarkers, Tumor/genetics
Carcinoma/diagnosis
Case-Control Studies
Colorectal Neoplasms/diagnosis
Feces/chemistry
Female
Gene Expression Profiling/methods
Humans
Male
MicroRNAs/genetics
Middle Aged
Molecular Diagnostic Techniques
Oligonucleotide Array Sequence Analysis
Predictive Value of Tests
ROC Curve
Real-Time Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
Up-Regulation

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10.18632/oncotarget.6403

Cite this

@article{6c65247aefb3431fb7644f7c1dbe36f3,

title = "MicroRNA-20a in human faeces as a non-invasive biomarker for colorectal cancer",

abstract = "OBJECTIVE: Detection of microRNA (miRNA) aberrations in human faeces is a new approach for colorectal cancer (CRC) screening. The aim of this study was to characterise miR-20a in faeces as a non-invasive biomarker for diagnosis of CRC.RESULTS: miR-20a expression was significantly higher in the 40 CRC tumours compared to their respective adjacent normal tissues (P = 0.0065). Levels of miR-20a were also significantly higher in faecal samples from CRC patients (P < 0.0001). The area under receiver operating characteristic (AUROC) curve for miR-20a was 0.73, with a sensitivity of 55\% and specificity of 82\% for CRC patients compared with controls. No significant difference in the level of miR-20a was found between patients with proximal, distal, and rectal cancer. The use of antibiotics did not influence faecal miR-20a levels.PATIENTS AND METHODS: miR-20a was selected from an expression microarray containing 667 miRNAs. Further verification of miR-20a was performed in 40 pairs of primary CRC tissues, as well as 595 faecal samples (198 CRCs, 199 adenomas, and 198 healthy controls) using TaqMan probe based quantitative Real-Time PCR (qRT-PCR).CONCLUSIONS: Faecal-based miR-20a can be utilised as a potential non-invasive biomarker for CRC screening.",

keywords = "Adenoma/diagnosis, Aged, Area Under Curve, Biomarkers, Tumor/genetics, Carcinoma/diagnosis, Case-Control Studies, Colorectal Neoplasms/diagnosis, Feces/chemistry, Female, Gene Expression Profiling/methods, Humans, Male, MicroRNAs/genetics, Middle Aged, Molecular Diagnostic Techniques, Oligonucleotide Array Sequence Analysis, Predictive Value of Tests, ROC Curve, Real-Time Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction, Up-Regulation",

author = "Yau, \{Tung On\} and Wu, \{Chung Wah\} and Ceen-Ming Tang and Yingxuan Chen and Jingyuan Fang and Yujuan Dong and Qiaoyi Liang and Ng, \{Simon Siu Man\} and Chan, \{Francis Ka Leung\} and Sung, \{Joseph Jao Yiu\} and Jun Yu",

year = "2016",

month = jan,

day = "12",

doi = "10.18632/oncotarget.6403",

language = "English",

volume = "7",

pages = "1559--68",

journal = "Oncotarget",

issn = "1949-2553",

publisher = "Impact Journals LLC",

number = "2",

}

TY - JOUR

T1 - MicroRNA-20a in human faeces as a non-invasive biomarker for colorectal cancer

AU - Yau, Tung On

AU - Wu, Chung Wah

AU - Tang, Ceen-Ming

AU - Chen, Yingxuan

AU - Fang, Jingyuan

AU - Dong, Yujuan

AU - Liang, Qiaoyi

AU - Ng, Simon Siu Man

AU - Chan, Francis Ka Leung

AU - Sung, Joseph Jao Yiu

AU - Yu, Jun

PY - 2016/1/12

Y1 - 2016/1/12

N2 - OBJECTIVE: Detection of microRNA (miRNA) aberrations in human faeces is a new approach for colorectal cancer (CRC) screening. The aim of this study was to characterise miR-20a in faeces as a non-invasive biomarker for diagnosis of CRC.RESULTS: miR-20a expression was significantly higher in the 40 CRC tumours compared to their respective adjacent normal tissues (P = 0.0065). Levels of miR-20a were also significantly higher in faecal samples from CRC patients (P < 0.0001). The area under receiver operating characteristic (AUROC) curve for miR-20a was 0.73, with a sensitivity of 55% and specificity of 82% for CRC patients compared with controls. No significant difference in the level of miR-20a was found between patients with proximal, distal, and rectal cancer. The use of antibiotics did not influence faecal miR-20a levels.PATIENTS AND METHODS: miR-20a was selected from an expression microarray containing 667 miRNAs. Further verification of miR-20a was performed in 40 pairs of primary CRC tissues, as well as 595 faecal samples (198 CRCs, 199 adenomas, and 198 healthy controls) using TaqMan probe based quantitative Real-Time PCR (qRT-PCR).CONCLUSIONS: Faecal-based miR-20a can be utilised as a potential non-invasive biomarker for CRC screening.

AB - OBJECTIVE: Detection of microRNA (miRNA) aberrations in human faeces is a new approach for colorectal cancer (CRC) screening. The aim of this study was to characterise miR-20a in faeces as a non-invasive biomarker for diagnosis of CRC.RESULTS: miR-20a expression was significantly higher in the 40 CRC tumours compared to their respective adjacent normal tissues (P = 0.0065). Levels of miR-20a were also significantly higher in faecal samples from CRC patients (P < 0.0001). The area under receiver operating characteristic (AUROC) curve for miR-20a was 0.73, with a sensitivity of 55% and specificity of 82% for CRC patients compared with controls. No significant difference in the level of miR-20a was found between patients with proximal, distal, and rectal cancer. The use of antibiotics did not influence faecal miR-20a levels.PATIENTS AND METHODS: miR-20a was selected from an expression microarray containing 667 miRNAs. Further verification of miR-20a was performed in 40 pairs of primary CRC tissues, as well as 595 faecal samples (198 CRCs, 199 adenomas, and 198 healthy controls) using TaqMan probe based quantitative Real-Time PCR (qRT-PCR).CONCLUSIONS: Faecal-based miR-20a can be utilised as a potential non-invasive biomarker for CRC screening.

KW - Adenoma/diagnosis

KW - Aged

KW - Area Under Curve

KW - Biomarkers, Tumor/genetics

KW - Carcinoma/diagnosis

KW - Case-Control Studies

KW - Colorectal Neoplasms/diagnosis

KW - Feces/chemistry

KW - Female

KW - Gene Expression Profiling/methods

KW - Humans

KW - Male

KW - MicroRNAs/genetics

KW - Middle Aged

KW - Molecular Diagnostic Techniques

KW - Oligonucleotide Array Sequence Analysis

KW - Predictive Value of Tests

KW - ROC Curve

KW - Real-Time Polymerase Chain Reaction

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Up-Regulation

U2 - 10.18632/oncotarget.6403

DO - 10.18632/oncotarget.6403

M3 - Article

C2 - 26621842

SN - 1949-2553

VL - 7

SP - 1559

EP - 1568

JO - Oncotarget

JF - Oncotarget

IS - 2

ER -

MicroRNA-20a in human faeces as a non-invasive biomarker for colorectal cancer

Abstract

UN SDGs

Keywords

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