Molecular phylogenetics of tsetse flies (Diptera: Glossinidae) based on mitochondrial (COI, 16S, ND2) and nuclear ribosomal DNA sequences, with an emphasis on the palpalis group

N A Dyer, S P Lawton, S Ravel, K S Choi, M J Lehane, A S Robinson, L M Okedi, M J R Hall, P Solano, M J Donnelly

Research output: Contribution to journalArticlepeer-review

50 Citations (Scopus)

Abstract

Relationships of 13 species of the genus Glossina (tsetse flies) were inferred from mitochondrial (cytochrome oxidase 1, NADH dehydrogenase 2 and 16S) and nuclear (internal transcribed spacer 1 of rDNA) sequences. The resulting phylogeny confirms the monophyly of the morphologically defined fusca, morsitans and palpalis subgenera. Genetic distances between palpalis and morsitans subspecies suggest that their status needs revision. In particular, cytochrome oxidase 1 sequences showed large geographical differences within G. palpalis palpalis, suggesting the existence of cryptic species within this subspecies. The morphology of palpalis group female genital plates was examined, and individuals were found varying outside the ranges specified by the standard identification keys, making definitive morphological classification impossible. A diagnostic PCR to distinguish G. palpalis palpalis, G. tachinoides and G. palpalis gambiensis based on length differences of internal transcribed spacer 1 sequences is presented.

Original languageEnglish
Pages (from-to)227-39
Number of pages13
JournalMolecular Phylogenetics and Evolution
Volume49
Issue number1
DOIs
Publication statusPrint publication - Oct 2008
Externally publishedYes

Keywords

  • Algorithms
  • Animals
  • DNA, Mitochondrial/genetics
  • DNA, Ribosomal/genetics
  • Female
  • Genes, Insect
  • Genes, Mitochondrial
  • Genetic Markers
  • Haplotypes
  • Likelihood Functions
  • Mitochondria/genetics
  • Phylogeny
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Species Specificity
  • Tsetse Flies/anatomy & histology

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